Haemonchus contortus hepatocellular carcinoma-associated antigen 59 with poly (lactic-co-glycolic acid): A promising nanovaccine candidate against Haemonchus contortus infection

Wang, Qiangqiang; Muhammad, Tahir Aleem; Muhammad, Waqqas Hasan; Muhammad, Ali; Haseeb, Muhammad; Ren, Yan; Xu, Lixin; Song, Xiaoling; Li, Xiangrui

doi:10.1016/j.vetpar.2021.109398

Cited by 6 publications

(4 citation statements)

References 51 publications

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“…In the process of preparing PLGA NPs (PLGA (lactic acid: glycolide 65:35, Molecular weight = 40,000–75,000, Sigma Aldrich, St. Louis, MO, USA)), it is imperative to ascertain the concentration of Polyvinyl Alcohol (PVA) (Molecular weight = 31,000–50,000, Sigma Aldrich, St. Louis, MO, USA) due to its crucial role as a constituent in PLGA [ 8 ]. Prior to commencing the preparation of nanoparticles, a comprehensive investigation was conducted to ascertain the most suitable concentration of polyvinyl alcohol (PVA) for optimal performance.…”

Section: Methodsmentioning

confidence: 99%

“…These factors include the inherent diversity within H. contortus, the genetic variability exhibited by the host, the life cycle specificity and distinct stages of the parasite, and the formulation of the vaccine [ 7 ]. Thus, there is substantial interest among scientists in developing new approaches, such as selective breeding and vaccinations with nanoparticles, as alternative methods of prevention against H. contortus infection [ 1 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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PLGA-Encapsulated Haemonchus contortus Antigen ES-15 Augments Immune Responses in a Murine Model

Hasan,

Ehsan,

Wang

et al. 2023

Vaccines

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Haemonchus contortus is a gastrointestinal parasite that adversely impacts small ruminants, resulting in a notable reduction in animal productivity. In the current investigation, we developed a nanovaccine by encapsulating the recombinant protein rHcES-15, sourced from the excretory/secretory products of H. contortus, within biodegradable poly (D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). The development of this nanovaccine involved the formulation of PLGA NPs using a modified double emulsion solvent evaporation technique. Scanning electron microscopy (SEM)verified the successful encapsulation of rHcES-15 within PLGA NPs, exhibiting a size range of 350–400 nm. The encapsulation efficiency (EE) of the antigen in the nanovaccine was determined to be 72%. A total of forty experimental mice were allocated into five groups, with the nanovaccine administered on day 0 and the mice euthanized at the end of the 14-day trial. The stimulation index (SI) from the mice subjected to the nanovaccine indicated heightened lymphocyte proliferation (*** p < 0.001) and a noteworthy increase in anti-inflammatory cytokines (IL-4, IL-10, and IL-17). Additionally, the percentages of T-cells (CD4+, CD8+) and dendritic cell phenotypes (CD83+, CD86+) were significantly elevated (** p < 0.01, *** p < 0.001) in mice inoculated with the nanovaccine compared to control groups and the rHcES-15 group. Correspondingly, higher levels of antigen-specific serum immunoglobulins (IgG1, IgG2a, IgM) were observed in response to the nanovaccine in comparison to both the antigenic (rHcES-15) and control groups (* p < 0.05, ** p < 0.01). In conclusion, the data strongly supports the proposal that the encapsulation of rHcES-15 within PLGA NPs effectively triggers immune cells in vivo, ultimately enhancing the antigen-specific adaptive immune responses against H. contortus. This finding underscores the promising potential of the nanovaccine, justifying further investigations to definitively ascertain its efficacy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PLGA-Encapsulated Haemonchus contortus Antigen ES-15 Augments Immune Responses in a Murine Model

Hasan,

Ehsan,

Wang

et al. 2023

Vaccines

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“…Furthermore, biodegradable solid lipids can be utilized to form SLN, which is supported by surfactants to maintain stability. Therefore, the SLN nanoparticle technology is an innovative approach for creating NE (oil‐in‐water) formulations by replacing the liquid lipid with a solid form, resulting in even greater stability (Wang et al., 2021 ). Hence, it is possible to regulate the release and balance of the system under environmentally harmful situations.…”

Section: Type Of Nanoparticles and Their Applicationsmentioning

confidence: 99%

Current status of nano‐vaccinology in veterinary medicine science

Sadr,

Poorjafari Jafroodi,

Haratizadeh

et al. 2023

Veterinary Medicine & Sci

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Vaccination programmes provide a safe, effective and cost‐efficient strategy for maintaining population health. In veterinary medicine, vaccination not only reduces disease within animal populations but also serves to enhance public health by targeting zoonoses. Nevertheless, for many pathogens, an effective vaccine remains elusive. Recently, nanovaccines have proved to be successful for various infectious and non‐infectious diseases of animals. These novel technologies, such as virus‐like particles, self‐assembling proteins, polymeric nanoparticles, liposomes and virosomes, offer great potential for solving many of the vaccine production challenges. Their benefits include low immunotoxicity, antigen stability, enhanced immunogenicity, flexibility sustained release and the ability to evoke both humoral and cellular immune responses. Nanovaccines are more efficient than traditional vaccines due to ease of control and plasticity in their physio‐chemical properties. They use a highly targeted immunological approach which can provide strong and long‐lasting immunity. This article reviews the currently available nanovaccine technology and considers its utility for both infectious diseases and non‐infectious diseases such as auto‐immunity and cancer. Future research opportunities and application challenges from bench to clinical usage are also discussed.

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“…However, these molecular mechanisms are yet to be explored and need further in-depth research. Therefore, to control parasitic infections researchers are actively exploring alternative methods such as nanovaccine ( 14 , 15 ), nanoparticle ( 16 ), and plant extracts have shown promising anti-parasitic activity to counter anthelminthic resistance in H. contortus ( 17–19 ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants

Ahmad,

Khan,

Majid

et al. 2024

Front. Vet. Sci.

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IntroductionHaemonchus contortus (H. contortus) is a blood-feeding nematode causing infectious disease haemonchosis in small ruminants of tropical and subtropical regions around the world. This study aimed to explore the prevalence and phylogeny of H. contortus in small ruminants using the internal transcribed spacer-2 (ITS-2) gene. In addition, a comprehensive review of the available literature on the status of H. contortus in Pakistan was conducted.MethodsFecal samples were collected from sheep and goats (n = 180). Microscopically positive samples were subjected to DNA extraction followed by PCR using species-specific primers.ResultsThe overall prevalence of H. contortus was 25.55% in small ruminants. The prevalence of H. contortus was significantly associated with months and area. The highest occurrence of haemonchosis was documented in July (38.70%), whereas the lowest occurred in December (11.11%), with significant difference. The prevalence was highest in the Ghamkol camp (29.4%) and lowest in the arid zone of the Small Ruminant Research Institute (17.5%) (p = 0.01). The results of the systematic review revealed the highest prevalence of haemonchosis (34.4%) in Khyber Pakhtunkhwa (p = 0.001).DiscussionPhylogenetic analysis revealed a close relationship between H. contortus and isolates from Asia (China, India, Iran, Bangladesh, Malaysia, and Mongolia) and European countries (Italy and the United Kingdom). It has been concluded that H. contortus is prevalent in small ruminants of Kohat district and all over Pakistan, which could be a potential threat to food-producing animals, farmers, dairy, and the meat industry. Phylogenetic analysis indicates that H. contortus isolates share close phylogenetic relationships with species from Asia and Europe.

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Haemonchus contortus hepatocellular carcinoma-associated antigen 59 with poly (lactic-co-glycolic acid): A promising nanovaccine candidate against Haemonchus contortus infection

Cited by 6 publications

References 51 publications

PLGA-Encapsulated Haemonchus contortus Antigen ES-15 Augments Immune Responses in a Murine Model

PLGA-Encapsulated Haemonchus contortus Antigen ES-15 Augments Immune Responses in a Murine Model

Current status of nano‐vaccinology in veterinary medicine science

Epidemiology and phylogeny of Haemonchus contortus through internal transcribed spacer 2 gene in small ruminants

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