Haemolymph [Na+] and [Cl-] loss in Gammarus fossarum exposed in situ to a wide range of acidic streams

Felten, Vincent; Guérold, François

doi:10.3354/dao061113

Cited by 12 publications

(7 citation statements)

References 44 publications

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“…Sodium concentrations in haemolymph were evaluated using atomic absorption spectrophotometry (Perkin Elmer Analyst 100) whereas chlorides were measured by ionic chromatography (Dionex 4500i equipped with an Ion Pac AS4A column). Further details on haemolymph sampling and analysis are available in [33].…”

Section: Methodsmentioning

confidence: 99%

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Behavioural and Physiological Responses of Gammarus pulex Exposed to Cadmium and Arsenate at Three Temperatures: Individual and Combined Effects

et al. 2012

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This study aimed at investigating both the individual and combined effects of cadmium (Cd) and arsenate (AsV) on the physiology and behaviour of the Crustacean Gammarus pulex at three temperatures (5, 10 and15°C). G. pulex was exposed during 96 h to (i) two [Cd] alone, (ii) two [AsV] alone, and (iii) four combinations of [Cd] and [AsV] to obtain a complete factorial plane. After exposure, survival, [AsV] or [Cd] in body tissues, behavioural (ventilatory and locomotor activities) and physiological responses (iono-regulation of [Na+] and [Cl−] in haemolymph) were examined. The interactive effects (antagonistic, additive or synergistic) of binary mixtures were evaluated for each tested temperature using a predictive model for the theoretically expected interactive effect of chemicals. In single metal exposure, both the internal metal concentration in body tissues and the mortality rate increased along metallic gradient concentration. Cd alone significantly impaired both [Na+] and [Cl−] while AsV alone had a weak impact only on [Cl−]. The behavioural responses of G. pulex declined with increasing metal concentration suggesting a reallocation of energy from behavioural responses to maintenance functions. The interaction between AsV and Cd was considered as ‘additive’ for all the tested binary mixtures and temperatures (except for the lowest combination at 10°C considered as “antagonistic”). In binary mixtures, the decrease in both ventilatory and locomotor activities and the decline in haemolymphatic [Cl−] were amplified when respectively compared to those observed with the same concentrations of AsV or Cd alone. However, the presence of AsV decreased the haemolymphatic [Na+] loss when G. pulex was exposed to the lowest Cd concentration. Finally, the observed physiological and behavioural effects (except ventilation) in G. pulex exposed to AsV and/or Cd were exacerbated under the highest temperature. The discussion encompasses both the toxicity mechanisms of these metals and their interaction with rising temperature.

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Section: Methodsmentioning

confidence: 99%

“…As a result, Na + and Cl − which are known to make up to 90% or more of the haemolymph osmotic pressure in most crustaceans [30], can be impaired by chemical stress (e.g. zinc [31]; copper [32]; cadmium [17]; acidification [33]). Osmoregulation is an important and highly energy-consuming regulatory function in aquatic invertebrates.…”

Section: Introductionmentioning

confidence: 99%

Behavioural and Physiological Responses of Gammarus pulex Exposed to Cadmium and Arsenate at Three Temperatures: Individual and Combined Effects

et al. 2012

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“…For the gammarids, after drying on filter paper, samples of haemolymph were taken from the telson of each individual (n=10) using a gauged 5-µl capillary. The capillaries were centrifuged for 5 min at 6,660 g. After centrifugation, the haemolymph volume was determined, diluted in 2 ml of MQ water and kept at 4°C before analysis (Felten and Guerold, 2004). Chloride concentrations were determined by ionic chromatography (Dionex 4500i equipped with a Ion Pac AS4A column) whereas sodium and calcium concentrations were measured by atomic absorption spectrometry (Perkin Elmer Analyst 100) (Felten et al, 2008).…”

Section: Analysis Of Haemolymph CL - Na + and Ca 2+ Concentrationsmentioning

confidence: 99%

Multibiomarker assessment of cerium dioxide nanoparticle (nCeO2) sublethal effects on two freshwater invertebrates, Dreissena polymorpha and Gammarus roeseli

et al. 2015

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“…Surprisingly, and despite the numerous papers reporting detrimental effects of acidification on macroinvertebrate communities, few realistic studies have been performed to assess the ecophysiology of smaller acid-sensitive species and finally, relatively little is known about their physiological responses to short-term acid stress. In previous studies (Felten & Guérold, 2001, 2004, we showed that Gammarus fossarum (Crustacea: Amphipoda), a common acidsensitive species in west Palearctica, also sustained a severe depletion of haemolymph Na + and Clions when exposed to acidic conditions. Conjointly, we determined relationship between acidification level and haemolymph ion losses (Felten & Guérold, 2004).…”

Section: Introductionmentioning

confidence: 95%

“…In previous studies (Felten & Guérold, 2001, 2004, we showed that Gammarus fossarum (Crustacea: Amphipoda), a common acidsensitive species in west Palearctica, also sustained a severe depletion of haemolymph Na + and Clions when exposed to acidic conditions. Conjointly, we determined relationship between acidification level and haemolymph ion losses (Felten & Guérold, 2004).…”

Section: Introductionmentioning

confidence: 95%

Short-term physiological responses to a severe acid stress in three macroinvertebrate species: A comparative study

Felten¹,

Guérold

2006

Chemosphere

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The present study focuses on the sensitivity among freshwater invertebrate species to acidic stress. Three common macroinvertebrate species in the Vosges Mountains (North-Eastern France), Gammarus fossarum (Amphipoda), Hydropsyche pellucidula (Trichoptera) and Dinocras cephalotes (Plecoptera) were exposed for 24, 72 and 120 h to natural acidified water (pH=4.73+/-0.08, [Ca2+]=39.1+/-0.6 micromol l(-1), [Al(tot)]=28.4+/-1 micromol l(-1)). Short-term exposure to acid stress caused significant decreases both in survival rate and haemolymph ions ([Cl-] and [Na+]). The relative sensitivity to a natural acidic stress slightly differed among the species and was in the following order: G. fossarum, as the most sensitive, then H. pellucidula and D. cephalotes. Results of this study confirm the interest of in situ tests to assess the toxicity of short-term acid exposure. Finally, our results reinforce the hypothesis that transient acidification can offset the recovery of sensitive species of macroinvertebrates in streams chemically recovering from acidification either through liming or declining deposition.

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Haemolymph [Na+] and [Cl-] loss in Gammarus fossarum exposed in situ to a wide range of acidic streams

Cited by 12 publications

References 44 publications

Behavioural and Physiological Responses of Gammarus pulex Exposed to Cadmium and Arsenate at Three Temperatures: Individual and Combined Effects

Behavioural and Physiological Responses of Gammarus pulex Exposed to Cadmium and Arsenate at Three Temperatures: Individual and Combined Effects

Multibiomarker assessment of cerium dioxide nanoparticle (nCeO2) sublethal effects on two freshwater invertebrates, Dreissena polymorpha and Gammarus roeseli

Short-term physiological responses to a severe acid stress in three macroinvertebrate species: A comparative study

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