Haemolymph microbiome of the cultured spiny lobster Panulirus ornatus at different temperatures

Ooi, Mei C.; Goulden, Evan F.; Smith, Gregory G.; Bridle, Andrew R.

doi:10.1038/s41598-019-39149-7

Cited by 22 publications

(23 citation statements)

References 71 publications

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“…Bacterial genera that decreased in abundance due to thermal stress included Loktanella , Polaribacter , Micrococcus and Cohaesibacter (Ooi et al . 2019).…”

Section: Microbiome and The Culture Environmentmentioning

confidence: 99%

Microbiome diversity and dysbiosis in aquaculture

Villamil

Huerlimann

Jerry

2020

Reviews in Aquaculture

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With the continuous growth of the human population and associated need for high‐quality protein, the aquaculture sector will be required to increase significantly in productivity. This growth in productivity will be achieved through more efficient use of resources like feeds, genetic improvement and limiting the impacts of disease. One of the key links between animal productivity and disease is that of microbial diversity, with high‐throughput sequencing technologies increasing our understanding of the role microorganisms play in health, development and physiology of vertebrate and invertebrate hosts alike. Increasing our understanding of microbial–host interactions will help avoid or manage dysbiosis in aquaculture systems with the final aim of improving productivity. We review the current literature, which indicates that there is an association between productivity and microbial diversity in aquaculture systems, as changes in bacterial microbiomes are implicated in animal performance, in disease development associated with both bacterial and viral origin, and in dysbiosis triggered by environmental stressors or diet choice. Dysbiosis, whether in the form of the loss of beneficial bacteria, or the expansion of pathogens or potentially harmful microorganisms, can be used as an indicator tool for productivity monitoring purposes. Development of management strategies towards preserving the microbial balance, including maintaining or increasing diversity in the host, is critical for the health of cultured aquatic animals and will likely be critical for the expansion of aquaculture.

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“…Bacterial genera that decreased in abundance due to thermal stress included Loktanella , Polaribacter , Micrococcus and Cohaesibacter (Ooi et al . 2019).…”

Section: Microbiome and The Culture Environmentmentioning

confidence: 99%

Microbiome diversity and dysbiosis in aquaculture

Villamil

Huerlimann

Jerry

2020

Reviews in Aquaculture

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“…Although temperature is important in lobster homeostasis, it may not be modulating diversity in the hemolymph of lobsters. In the tropical ornate spiny lobster Panulirus ornatus, a 6 d increase to 34°C (+ 6°C above ambient) had no effect on hemolymph microbiomes (OTUs, richness, diversity indices) compared to those from animals held at ambient temperature (28°C), even the though those held at 34°C began to exhibit mortality after 4 d (Ooi et al 2019). Given that lobsters and other crustaceans are often held at somewhat elevated temperatures for commercial shipment, additional studies are needed to determine how temperature affects hemolymph microbiome of these crustaceans.…”

Section: Discussionmentioning

confidence: 94%

“…Members of the Rhodobacteraceae are aquatic photo-and chemoheterotrophs as well as symbionts in other organisms (Pujalte et al 2014). Rhodobacteraceae has been reported as changing in richness in marine organisms in relation to temperature (Stratil et al 2013, Ooi et al 2019, but the role of this taxon in the lobster hemolymph is unknown. Methylobacterium spp.…”

Section: Discussionmentioning

confidence: 99%

“…Quinn et al (2013) examined bacteria in the lobster hemolymph using culture-independent methods (nested-PCR of 16S rRNA and denaturing gradient gel electrophoresis), but reported only a few taxa, and one sample did not have bacteria. In a study using next generation sequencing (NGS) of 16S amplicons, the whole hemolymph mi cro biome of the spiny lobster Panulirus ornatus was found to include a high diversity of potentially symbiotic bacteria (Ooi et al 2019). Given that spiny lobsters (Infraorder Palinura: Palinuridae) are not clo sely related to clawed lobsters (Infraorder Asta cidea: Nephropidae), and live in tropical and subtropical regions, they are likely to have very different microbiomes.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of the hemolymph microbiome between live and recently dead American lobsters Homarus americanus

et al. 2021

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Lobsters and other crustaceans do not have sterile hemolymph. Despite this, little is known about the microbiome in the hemolymph of the lobster Homarus americanus. The purpose of this study was to characterize the hemolymph microbiome in lobsters. The lobsters were part of a larger study on the effect of temperature on epizootic shell disease, and several died during the course of the study, providing an opportunity to examine differences in the microbiomes between live and recently dead (1-24 h) animals. The hemolymph microbiomes of live lobsters was different from those in dead animals and both were different from the tank microbiome in which the animals had been held. The microbiomes of live lobsters were more diverse and had a different suite of bacteria than those from dead animals. The dominant taxa in live lobsters belonged to Flavobacteriaceae and Rhodobacteraceae, whereas Vibrionaceae and Enterobacteriaceae were predominant in the dead lobsters. Although aquarium microbiomes overlapped with the hemolymph microbiomes, there was less overlap and lower abundance of taxa in comparison with hemolymph from live lobsters. Previous studies reporting bacteria in the digestive tract of lobsters suggested that Vibrionaceae and Enterobacteriaceae had invaded the hemolymph via the gut. Our study suggests that hemolymph bacteria abundant in live lobsters do not originate from the tank milieu and comprise a rich, natural, or native background of bacterial constituents.

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“…Some of these phyla have been described also as predominant bacterial groups in other marine invertebrates. For example, Proteobacteria and/or Bacteroidetes are dominant phyla in the hemolymph of the mussel Mytilus coruscus ( 47 ), the oyster Crassostrea gigas ( 48 ), certain crustaceans ( 49 ), and in gonads from the scallop Pecten maximus ( 50 ). Yet, high variability in bacterial community composition and the lack of a common bacterial core between all scallop individuals is more evident at the class and genera level.…”

Section: Discussionmentioning

confidence: 99%

Host Defense Effectors Expressed by Hemocytes Shape the Bacterial Microbiota From the Scallop Hemolymph

et al. 2020

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The interaction between host immune response and the associated microbiota has recently become a fundamental aspect of vertebrate and invertebrate animal health. This interaction allows the specific association of microbial communities, which participate in a variety of processes in the host including protection against pathogens. Marine aquatic invertebrates such as scallops are also colonized by diverse microbial communities. Scallops remain healthy most of the time, and in general, only a few species are fatally affected on adult stage by viral and bacterial pathogens. Still, high mortalities at larval stages are widely reported and they are associated with pathogenic Vibrio . Thus, to give new insights into the interaction between scallop immune response and its associated microbiota, we assessed the involvement of two host antimicrobial effectors in shaping the abundances of bacterial communities present in the scallop Argopecten purpuratus hemolymph. To do this, we first characterized the microbiota composition in the hemolymph from non-stimulated scallops, finding both common and distinct bacterial communities dominated by the Proteobacteria, Spirochaetes and Bacteroidetes phyla. Next, we identified dynamic shifts of certain bacterial communities in the scallop hemolymph along immune response progression, where host antimicrobial effectors were expressed at basal level and early induced after a bacterial challenge. Finally, the transcript silencing of the antimicrobial peptide big defensin ApBD1 and the bactericidal/permeability-increasing protein ApLBP/BPI1 by RNA interference led to an imbalance of target bacterial groups from scallop hemolymph. Specifically, a significant increase in the class Gammaproteobacteria and the proliferation of Vibrio spp. was observed in scallops silenced for each antimicrobial. Overall, our results strongly suggest that scallop antimicrobial peptides and proteins are implicated in the maintenance of microbial homeostasis and are key molecules in orchestrating host-microbiota interactions. This new evidence depicts the delicate balance that exists between the immune response of A. purpuratus and the hemolymph microbiota.

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Haemolymph microbiome of the cultured spiny lobster Panulirus ornatus at different temperatures

Cited by 22 publications

References 71 publications

Microbiome diversity and dysbiosis in aquaculture

Microbiome diversity and dysbiosis in aquaculture

Comparative study of the hemolymph microbiome between live and recently dead American lobsters Homarus americanus

Host Defense Effectors Expressed by Hemocytes Shape the Bacterial Microbiota From the Scallop Hemolymph

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