Fatty acid composition and distribution in edible species of fish and shellfish captured in the South Pacific were studied, with a focus on n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA). Fatty acids were quantified using gas-chromatography coupled with flame ionization detection (GC-FID), and the distribution of different fatty acids within lipid classes (neutral and polar lipids) was achieved after oil extraction using the Folch method and separation of lipid classes via solid-phase extraction for further GC-FID analysis. Red cusk-eel was the fish species with the lowest content of both EPA and DHA (40.8 and 74.4 mg/100 g, respectively) whereas mackerel contained the highest amount (414.7 and 956.0 mg/100 g for EPA and DHA, respectively). Sea squirt was the shellfish species with the highest content of EPA and DHA (375.0 and 165.7 mg/100 g, respectively) whereas the lowest amount of EPA + DHA was found in Chilean abalone (63.6 mg/100 g). PUFA were mostly found in neutral or polar lipids depending on the studied species. Indexes used to discuss the nutritional quality of lipids (PUFA/SFA, n-6/n-3 ratio and the hypocholesterolemic/hypercholesterolemic fatty acid index) were calculated and reported in the manuscript. This information provides a novel nutritional insight which may be useful to help nutritionists and other health professionals give more accurate counseling for the population to reach the recommended daily intakes of EPA and DHA.
Florfenicol is an important antibiotic in veterinary medicine that is used extensively in aquaculture, including salmon farming in Chile. We analysed a set of 119 florfenicol-resistant Gram-negative bacilli from seven freshwater Chilean salmon farms for the molecular determinants involved in the florfenicol resistance. Ninety-seven of these strains were glucose non-fermenting bacilli, mainly belonging to the Pseudomonas genus, whereas 22 strains were glucose-fermenters. The floR gene was detected in 26 strains (21.8%) that had been isolated from three of the seven salmon farms. Most of the floR-carrying strains were glucose fermenters (21 strains), and most of the floR-carrying strains were also resistant to streptomycin, chloramphenicol and oxytetracycline. The minimum inhibitory concentrations against florfenicol were assessed in the presence and absence of the efflux pump inhibitor Phe-Arg-beta-naphthylamide (MC-207,110). There was evidence that in the majority of non-fermenting bacteria (82 strains), florfenicol resistance was at least partially mediated by non-specific efflux pump systems. Given the diversity of antibiotic resistance patterns observed in this study in the floR-positive isolates, a single antibiotic has the potential to co-select for a diversity of resistances. For this reason, human health as well as animal health can potentially be impacted by the use of antibiotics in aquaculture. To assess this potential risk, future studies should focus on the ability of different antibiotics used in aquatic environments to co-select for multiple resistances, the molecular basis of this diversity of resistance, and whether the genes conferring resistance can be transferred to other bacteria, including those of human health concern.
AJ, Reyes RV. Store-operated channels in the pulmonary circulation of high-and low-altitude neonatal lambs. Am J Physiol Lung Cell Mol Physiol 304: L540 -L548, 2013. First published February 15, 2013 doi:10.1152/ajplung.00024.2012.-We determined whether store-operated channels (SOC) are involved in neonatal pulmonary artery function under conditions of acute and chronic hypoxia, using newborn sheep gestated and born either at high altitude (HA, 3,600 m) or low altitude (LA, 520 m). Cardiopulmonary variables were recorded in vivo, with and without SOC blockade by 2-aminoethyldiphenylborinate (2-APB), during basal or acute hypoxic conditions. 2-APB did not have effects on basal mean pulmonary arterial pressure (mPAP), cardiac output, systemic arterial blood pressure, or systemic vascular resistance in both groups of neonates. During acute hypoxia 2-APB reduced mPAP and pulmonary vascular resistance in LA and HA, but this reduction was greater in HA. In addition, isolated pulmonary arteries mounted in a wire myograph were assessed for vascular reactivity. HA arteries showed a greater relaxation and sensitivity to SOC blockers than LA arteries. The pulmonary expression of two SOC-forming subunits, TRPC4 and STIM1, was upregulated in HA. Taken together, our results show that SOC contribute to hypoxic pulmonary vasoconstriction in newborn sheep and that SOC are upregulated by chronic hypoxia. Therefore, SOC may contribute to the development of neonatal pulmonary hypertension. We propose SOC channels could be potential targets to treat neonatal pulmonary hypertension.hypoxia; pulmonary vasoconstriction; pulmonary hypertension; 2-aminoethydiphenylborinate; pulmonary vascular reactivity PULMONARY ARTERIES HAVE AN intrinsic vasoconstrictor response to low oxygen levels when exposed to acute hypoxia. This is a reversible, rapid and physiological response, known as hypoxic pulmonary vasoconstriction (HPV), that redirects blood flow from poorly oxygenated to better oxygenated alveoli. Thus, when total lung is exposed to hypoxia, HPV results in an increase in pulmonary artery pressure (PAP) that reverses when normoxia is reestablished (31). However, exposure to chronic hypoxia produces an imbalance between vasodilator and vasoconstrictor mechanisms, and there is pulmonary vascular remodeling that includes proliferation of pulmonary artery myocytes among other cellular processes (46). The result is a pathological and persistent increase in pulmonary artery contractile tone and pulmonary arterial hypertension, which in many cases leads to right ventricular hypertrophy, right heart failure, and eventually death (13).In pulmonary artery smooth muscle cells, an increase in intracellular calcium concentration ([Ca 2ϩ ] i ) is essential for HPV, proliferation, and remodeling (13,19,20,42). This increase in [Ca 2ϩ ] i greatly depends on an influx of extracellular calcium (13, 59), which may enter the smooth muscle cell through store-operated channels (SOC) among other pathways (5,8,22,40). These are channels physiologically ...
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