Haemoglobin GCopenhagen and haemoglobin JCambridge. Two new β-chain variants of haemoglobin A

Haemoglobin J Baltimore (α₂ β₂^{16 gly}^→as^p) and Haemoglobin D punjab (α₂β₂^{121 glu→gl}ⁿ) in Two Dutch Families

“…The original Hb D described by Itano [1951], in a Caucasian family in Los Angeles, was shown by Babin et al [1964] to have the same amino acid substitution. This abnormal haemoglobin has also been found in an Englishman [H untsman et al, 1963], in a Danish subject [Sick et al, 1967], probably in three Italian families [only fingerprinted, Ventruto et al, 1963] and now in a Dutch family. This haemoglobin is also known as Hb D Los Angeles, D Cyprus and D Chicago.…”

Hb J sicilia: P 65 (E9) Lys→Asn, a beta homologue of Hb zambia

“…Chromatographic separation on a Sephadex DEAE A-50 column [3] was followed by vacuum concentration, incubation with parachloromercuribenzoate (PCMB) [4] and electrophoretic separation of the alpha and beta subunits. Globin obtained by cold precipitation with HCl-acetone from the abnormal fraction was digested with trypsin and fingerprinted [5,6]. Fingerprints were also obtained from the alpha and beta chains, following their separation with urea and flmercaptoethanol [7] and amino-ethylation [8].…”

A new haemoglobin J from Turkey ‐ HB Ankara (β 10 (A7) Ala → Asp).

“…Haemolysates were fractionated using column chromatography as described by Huisman and Dozy [3] ; the resulting haemoglobin fractions were concentrated by ultrafiltration at 4°C and purified by paper electrophoresis [1 ], Globin was prepared from purified haemoglobin by acid-acetone precipitation at -20°C [4], washed three times with cold acetone and dried in vacuo over P2Os. Digestion of the total globin with trypsin and analysis of that portion of the digest which was soluble at pH 6.4, by fingerprinting, was carried out as previously described [5]. The abnormal peptide from Hb Ankara was isolated by preparative fingerprinting followed by paper electrophoresis at pH 3.5 (55 V/cm for 2hr), located by staining with dilute (0.02%, w/v) ninhydrin in acetone (containing 1% v/v pyridine) and eluted with 0.5 N NH4OH.…”