Haemodialysis monocytopenia: differential sequestration kinetics of CD14+CD16+ and CD14++ blood monocyte subsets

Abstract

SUMMARYIn peripheral blood the majority of circulating monocytes present a CD14 high CD162 (CD14 11 ) phenotype, while a subpopulation shows a CD14 low CD16 1 (CD14 1 CD16 1 ) surface expression. During haemodialysis (HD) using cellulosic membranes transient leukopenia occurs. In contrast, synthetic biocompatible membranes do not induce this effect. We compared the sequestration kinetics for the CD14 1 CD16 1 and CD14 11 monocyte subsets during haemodialysis using biocompatible dialysers. Significant monocytopenia, as measured by the leucocyte count, occurred only during the first 30 min. However, remarkable differences were observed between the different monocyte subsets. CD14 11 monocyte numbers dropped to 77^13% of the predialysis level after 15 min, increasing to $ 93% after 60 min. In contrast, the CD14 1 CD16 1 subset decreased to 33^15% at 30 min and remained suppressed for the course of dialysis (67^11% at 240 min). Approximately 6 h after the end of HD the CD14 1 CD16 1 cells returned to basal levels. Interestingly, the CD14 1 CD16 1 monocytes did not show rebound monocytosis while a slight monocytosis of CD14 11 monocytes was occasionally observed during HD. A decline in CD11c surface density paralleled the sequestration of CD14 1 CD16 1 monocytes. Basal surface densities of important adhesion receptors differed significantly between the CD14 1 CD16 1 and CD14 11 subsets. In conclusion, during HD the CD14 1 CD16 1 subset revealed different sequestration kinetics, with a more pronounced and longer disappearance from the blood circulation, compared with CD14 11 monocytes. This sequestration kinetics may be due to a distinct surface expression of major adhesion receptors which facilitate leucocyte±leucocyte, as well as leucocyte±endothelial, interactions.

Standardized single‐platform assay for human monocyte subpopulations: Lower CD14+CD16++ monocytes in females

“…11 monocytes was noted during hemodialysis therapy (27). In many of these studies, percentages of monocyte subsets were reported and when absolute numbers were determined then they were derived from two-platform approaches with its inherent imprecision.…”

“…A group of 11 healthy individuals (age [18][19][20][21][22][23][24][25][26][27][28][29][30] were studied before and after physical exercise on a home-trainer bike at 200 watts (W) either for 1 min or to exhaustion for 3-10 min. In addition, we analyzed four patients with inflammatory diseases.…”

CD14CD16 Monocyte Subset Levels in Heart Failure Patients

“…+ together with low levels of CD14 + was expanded in dialysis patients [4]. It has recently been shown that CD16 + monocytes are further differentiated by phenotype into CD14…”

CD16+Monocyte Subsets Are Increased in Large Abdominal Aortic Aneurysms and Are Differentially Related with Circulating and Cell-Associated Biochemical and Inflammatory Biomarkers

“…Most of monocytes from healthy subjects are CD14 + CD16 − ; CD16 + monocytes increase their frequency in response to chronic inflammatory conditions, such as chronic kidney disease (CKD). Nockher reported that the subset of monocytes expressing CD16 together with low levels of CD14 was expanded in dialysis patients [12]. More recently, it has been shown that CD16…”