Haematological response of curimbas Prochiloduslineatus, naturally infected with Neoechinorhynchuscuremai

Belo, Marco Antônio de Andrade; Souza, D. G. F.; Faria, Vanessa Pavesi de; Prado, E. J. R.; Moraes, Flávio Ruas de; Onaka, Eduardo Makoto

doi:10.1111/jfb.12060

Cited by 25 publications

(24 citation statements)

References 11 publications

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“…Juveniles and adults of cachama under moderate to severe infection by N. buttnerae have shown hyperaemia, hardening and thickening of the intestinal tissue, and yellowish mucosal secretion of a bluetongue aspect. Such alterations were observed in lower intensity in cachama juveniles, which was associated with low parasitic burden, similarly to that previously reported (Belo et al, 2013). These authors found a positive correlation between the size of fish and number of acanthocephalans and consequently pathological alterations in curimbatá (Prochilodus lineatus Valenciennes) naturally infected by N. curemai Noronha, 1973.…”

Section: Discussionsupporting

confidence: 86%

“…A significant increase in the number of monocytes and a decrease in the number of thrombocytes in curimbatá (P. lineatus) infected by N. curemai (Belo et al, 2013) and pacu (Piaractus mesopotamicus) with chronic inflammatory reaction (Belo et al, 2014) justify the present findings, which showed intense inflammatory reaction composed mainly of macrophages. In fact, the chronic aspect of intestinal lesions in cachama with participation of macrophages and polycarion formation on the inflamed site is the result of the recruitment of monocytes from circulating blood.…”

Section: Discussionsupporting

confidence: 78%

“…As a result of the acanthocephalan proboscis attachment on the intestinal tissue, the fishes' health and pathological alterations (Belo et al, 2013;Melo et al, 2014) interfere in the nutrient absorption and affect fish growth. In tropical cachama farming this kind of parasitism has been considered the main obstacle responsible for economic losses and high feeding costs.…”

Section: Introductionmentioning

confidence: 99%

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Neoechinorhynchus buttnerae (Acanthocephala) infection in farmed Colossoma macropomum: A pathological approach

Jerônimo

Pádua²,

Belo

et al. 2017

Aquaculture

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Infection by the acanthocephalan Neoechinorhynchus buttnerae is considered the most important obstacle in farmed tambaqui or cachama (Colossoma macropomum), the second most cultivated fish species in Brazil. This study describes the occurrence of N. buttnerae in farmed this fish based on parasitological and histopathological analysis in highly infected fish. A total of 102 fish were collected from fish farms located in Brazilian states in the northern region. All of the examined fish were heavily infected by N. buttnerae and showed no signs of anorexia, but heterogeneous growth and cachexia were frequently found. No cases of mortality were observed in fish farms. Macroscopically, the intestines showed hyperaemia, hardening, thickening of the intestinal wall, yellowish mucosal secretion and a bluetongue aspect. Histological sections showed different degrees of penetration of the acanthocephalan proboscis in the intestinal tissue, surpassing the mucosa, submucosa and muscular layer, provoking dilacerations due to their spines as well as an intense inflammatory reaction composed of macrophages, Langerhans cells and lymphocytes, and granuloma formation located mainly in the submucosa layer. Submucosal oedema, an increased number of goblet cells, metaplasia areas and a reduction of the intestinal villi were also observed. The results present an important register of N. buttnerae infection in C. macropomum and contribute to the comprehension of pathogeny caused by these worms. Statement of relevance: Characid fish farming has led to significantly increased fish production in Brazil. However, the most important obstacle is the acanthocephalan infection caused by Neoechinorhynchus buttnerae in the intestinal tract. The infection does not provoke death but cachexia and growth reduction are frequently observed as well as intense pathological conditions in histological sections.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

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Neoechinorhynchus buttnerae (Acanthocephala) infection in farmed Colossoma macropomum: A pathological approach

Jerônimo

Pádua²,

Belo

et al. 2017

Aquaculture

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“…Blood extensions were prepared for differential leukocyte count, stained with May-Grünwald-Giemsa-Wright. 200 cells were counted by extension to determine the percentage of each cell type [14].…”

Section: Hematologymentioning

confidence: 99%

Clinical safety of amino acids and vitamins administered through parenteral route

Moraes¹,

Scherma²,

Prado³

et al. 2017

Int J Vet Sci Res

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The parenteral use of amino acids and vitamins has been used to improve performance in animal production. However, few studies demonstrating the toxicity of these compounds. The present investigation evaluated the toxicological and clinical safety of the injectable supplement compound by amino acids and vitamins, administered by intramuscular route in Wistar rats. 56 females (± 220g), pubescent virgins, were randomly divided into seven groups (n=8): Treated with dose of 0.2, 2 and 4 mL, sampled 24 and 96 hours post-treatment (HPT), totaling six groups and one control group (Physiology Standard). Signifi cant increase in the enzymatic activity of serum ALT, AST, LDH and ALP was observed in animals treated with 2 and 4 mL (24 HPT). However, these changes returned to baseline levels 96 HPT, except in serum AST and ALP in animals treated with 4 mL. CK-NAC showed dose-response effect, since the higher compound dose was followed by proportional increase in the circulating levels of this enzyme, associated to muscle degeneration and necrosis area in rats treat with 4mL at 96 HPT. The evaluation of hepatic function did not result in signifi cant changes in the serum levels of triglycerides, cholesterol, albumin, urea and creatinine in the comparison between rats treated with 0.2 mL and control rats, demonstrating the innocuousness of this treatment on the activity of liver and kidney. However, deleterious effects were observed in animals subjected to doses of 2 and 4 mL, including observed increased splenic and hepatosomatic response, signifi cant decrease in circulating albumin levels and signifi cant leukocytosis, which is characterized by neutrophilia, followed by lymphopenia demonstrating the compound irritating effect on muscle tissue.

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“…Also, it is know that fish blood parameters can be influenced by several factors, such as seasonal variations, genetics, age, gender, stock density, stress and water quality (Ekanem et al 2012, Belo et al 2013, Castro et al 2014a, what could explain the lack of information on the reference values of health and ill fish.…”

Section: Introductionmentioning

confidence: 99%

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

Farias¹,

Pereira²,

Pádua³

et al. 2016

Pesq. Vet. Bras.

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RESUMO.-[Anticoagulante Na 2 EDTA danifica amostras de sangue do teleósteo Piaractus mesopotamicus.] O presente estudo teve por objetivo avaliar os efeitos da heparina sódica e Na 2 EDTA no sangue de Piaractus mesopotamicus (360,7±42,4g, 26,4±1,0cm). Foram amostrados vinte peixes em dois ensaios experimentais, sendo dez peixes utilizados para análise da fragilidade dos eritrócitos e dez peixes para análise dos parâmetros hematológicos e estudo bioquímico do plasma. O sangue coletado por punção veno-caudal foi aliquotado e armazenado em diferentes soluções anticoagulantes: Na 2 EDTA 10%, Na 2 EDTA 3%, heparina sódica 5.000 UI e heparina sódica 100 UI. Níveis plasmáticos de cálcio apresentados nas amostras armazenados em Na 2 EDTA diminuíram cerca de 80% em relação aos dois grupos armazenados com heparina. Amostras de sangue de pacus armazenados com Na 2 EDTA demonstraram aumento do hematócrito e VCM, e diminuição na CHCM. O efeito dose-resposta foi observado neste estudo. Estes resultados são reforçados pelos níveis mais elevados de proteína plasmática e hemólise apresentado no sangue armazenado com Na 2 EDTA 10%, o que confirma o efeito deletério desse tratamento anticoagulante na qualidade de amostras de sangue. Na 2 EDTA não é indicada para armazenar amostras de sangue de P. mesopotamicus, e heparina sódica a 100 UI é o anticoagulante mais recomendado, uma vez que este tratamento apresentou a menor taxa de alterações no sangue armazenado. The present study aimed to evaluate the effects of Na heparin and Na 2 EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na 2 EDTA 10%, Na 2 EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na 2 EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na 2 EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na 2 EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na 2 EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. INDEX TERMS: Fish hematology, blood clotting, EDTA, Heparin, Piaractus mesopotamicus.

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Haematological response of curimbas Prochiloduslineatus, naturally infected with Neoechinorhynchuscuremai

Cited by 25 publications

References 11 publications

Neoechinorhynchus buttnerae (Acanthocephala) infection in farmed Colossoma macropomum: A pathological approach

Neoechinorhynchus buttnerae (Acanthocephala) infection in farmed Colossoma macropomum: A pathological approach

Clinical safety of amino acids and vitamins administered through parenteral route

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

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