Haematological Changes during Fracture Healing in Goats

Kumar, Dharmendra; Bhargava, M.K.; Singh, Randhir; Shahi, Apra; Aparajita, Jahnawi; Swami, Madhu; Jain, Shyama; Rao, Malla Bhaskara; Singh, Ajit Pal

doi:10.9790/2380-0909010103

Cited by 3 publications

(15 citation statements)

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“…Since blood is a reflector of the health status of animals, responses to exposure to foreign bodies, and the state of disease in animals, 1 2 3 4 5 6 7 we used hematology to evaluate the responses of the experimental rabbits to the grafted caprine DBM, which is expected to initiate some antigenicity. Several reports have documented normal hematological parameters in rabbits in different geographical zones and conditions, 4 7 8 16 but the fluctuation within the normal physiological ranges make its use in diagnosis difficult, 9 especially in rabbits, in which gender, diurnal rhythm, breed, ambient temperature, age, and stress have been reported to affect their haematology. 7 8 16 It is, therefore, imperative to compare hematological responses with the baseline in any experimental condition, as seen in other studies.…”

Section: Discussionmentioning

confidence: 99%

“…The increase or decrease of basophils, monocytes, and eosinophils could be subjective in fracture healing monitoring. 9 Due to the scarcity of information on hematological response of fracture healing in animal models, we, therefore, report in the present study, the observed hematological changes in rabbits that underwent xenografting of caprine demineralized bone matrix (CDBM).…”

Section: Introductionmentioning

confidence: 97%

“…In fracture healing, monitoring of hematological parameters play a vital role as any deviation from the physiological reference range could be an indication of infection or graft response and, therefore, require urgent attention to avert the possibility of mal-union, delayed union, or non-union. 9 Hematology is, however, characterized by fluctuation within the normal physiological ranges, which may make its use in diagnosis difficult. 9 Decrease in total RBC is seen following trauma and surgery.…”

Section: Introductionmentioning

confidence: 99%

“… 9 Hematology is, however, characterized by fluctuation within the normal physiological ranges, which may make its use in diagnosis difficult. 9 Decrease in total RBC is seen following trauma and surgery. 9 Stress, trauma, and surgery are known to cause leukocytosis, lymphocytopenia, and neutrophilia, which could also fluctuate within normal ranges after the initial problem subsides.…”

Section: Introductionmentioning

confidence: 99%

“… 9 Decrease in total RBC is seen following trauma and surgery. 9 Stress, trauma, and surgery are known to cause leukocytosis, lymphocytopenia, and neutrophilia, which could also fluctuate within normal ranges after the initial problem subsides. The increase or decrease of basophils, monocytes, and eosinophils could be subjective in fracture healing monitoring.…”

Section: Introductionmentioning

confidence: 99%

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Alterações hematológicas após implante de matriz óssea desmineralizada caprina em modelo de defeito ulnar crítico em coelhos

Alimi

Abubakar

Yakubu

et al. 2021

Rev Bras Ortop (Sao Paulo)

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Resumo Objetivo Diversos modelos animais têm sido usados em estudos sobre enxertos ósseos e o tratamento de fraturas, mas as respostas hematológicas são raramente relatadas. Este estudo descreveu as alterações hematológicas observadas em coelhos submetidos a xenoenxertos de matriz óssea desmineralizada caprina (MODC). Métodos Vinte e quatro (24) coelhos machos (2,5 ± 0,5 kg) foram adquiridos para este estudo e divididos aleatoriamente em três grupos: enxerto ósseo autólogo (EOA); controle negativo sem preenchimento (SP) e matriz óssea desmineralizada caprina (MODC). Amostras de sangue foram coletadas por punção cardíaca sob anestesia com xilazina-quetamina no dia 0 (para estabelecimento dos valores basais) e aos dias 28 e 56 após a cirurgia; essas amostras foram submetidas à análise manual em até 2 horas após a coleta. A análise estatística foi composta por análise de variância (ANOVA) de dois fatores com medidas repetidas, e o valor de p < 0,05 foi considerado significativo. Resultados Houve uma diferença geral significativa nos números de leucócitos totais (p = 0,0043), neutrófilos (p < 0,0001), monócitos (p = 0,0184) e hemácias (p = 0,003), na concentração de hemoglobina (p < 0,0001) e no hematócrito (p < 0,0001) ao longo dos dias e entre os grupos de tratamento. No entanto, não houve diferença global significativa no número de linfócitos (p = 0,4923), basófilos (p = 0,4183) e eosinófilos (p = 0,4806) entre os dias. Conclusão A resposta ao enxerto de MODC em coelhos é, portanto, caracterizada por leucocitose intensa com neutrofilia, linfocitose e monocitose no 28° dia após o procedimento. Esses dados podem basear a utilização da hematologia no monitoramento da resposta corporal em modelos animais de enxerto ósseo.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Alterações hematológicas após implante de matriz óssea desmineralizada caprina em modelo de defeito ulnar crítico em coelhos

Alimi

Abubakar

Yakubu

et al. 2021

Rev Bras Ortop (Sao Paulo)

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Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

Nath

Sarma

2022

IJAR

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Background: The surgical reconstruction of large abdominal defects with application of surgical mesh of synthetic origin resulted a number of potential clinical complications; although it is having good tensile strength. Acellular bio-mesh derived from slaughtered animals could be better alternative for surgical management abdominal wall defects. The present study aimed to evaluate bio-detergent based decellularized allogenic porcine acellular diaphragm matrix in umbilical hernioplasty in pig. Methods: For the present study sodium deoxycholate was used as bio-detergent for preparation of porcine allogenic acellular diaphragm matrix (ADM). All total eighteen (18) numbers of piglets aged 2-3 months, body weight 8-14 kg and either sex with umbilical hernia equally divided in to three groups. Group A was subjected to application of ADM; while in Group B injection of methylprednisolone along with ADM application and Autologous Tunica Vaginalis was used for hernioplasty in Group C. Various parameters viz clinical, gross evaluation of surgical wound, haemato-biochemical and indirect ELISA of blood and serum was carried out as per standard methods. Result: Decellularization protocol carried out with sodium deoxycholate as bio-detergent@ 2% concentration resulted complete decellularization of porcine diaphragm and preserved the distinctive, natural, three-dimensional collagen structure within the prepared matrix. Clinical and haemato-biochemical observations following umbilical hernioplasty in pig with ADM promises its clinical utility. The immune response of host tissue to bio-mesh as revealed by Indirect ELISA was negligible.

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Xenogenic Acellular Pericardium Matrix of Caprine and Porcine Origin for Abdominal Wall Reconstruction in Rabbits

Padhan¹,

Nath²,

Sarma³

et al. 2022

IJAR

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Background: The abdominal wall defects require urgent medical attention owing to the voluminous visceral content and uneven wound edges, which remains a clinical challenge for the surgeon. In the present study, xenogenic porcine and caprine acellular pericardium matrix was evaluated for surgical reconstruction of the induced abdominal wall in rabbits. Methods: All total eighteen adult rabbits were divided into three equal groups. The defects were corrected with acellular caprine and porcine pericardium matrix in Group A and B, respectively and Group C was kept as control. Various parameters viz clinical, gross evaluation of surgical wound, degree of adhesion and histopathological changes were evaluated as per standard methods. Result: Clinical parameters and gross changes of the surgical wound showed significant changes on the 3rd and 5th day in all the groups. TLC, Serum Alkaline Phosphatase and Total Protein showed significant variation in Group A and B on the 10th day. Macroscopic and histopathological evaluation of implanted mesh has shows better host tissue proliferation in both the groups. Acellular caprine and porcine pericardium can be good options as bio-mesh for the repair of the abdominal wall and the subject may be considered for in-depth studies and further research programs.

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Haematological Changes during Fracture Healing in Goats

Cited by 3 publications

References 4 publications

Alterações hematológicas após implante de matriz óssea desmineralizada caprina em modelo de defeito ulnar crítico em coelhos

Alterações hematológicas após implante de matriz óssea desmineralizada caprina em modelo de defeito ulnar crítico em coelhos

Preparation of Porcine Allogenic Acellular Diaphragm Matrix and its Application for Umbilical Hernioplasty in Pig

Xenogenic Acellular Pericardium Matrix of Caprine and Porcine Origin for Abdominal Wall Reconstruction in Rabbits

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