Haematological and biochemical studies on Justicia carnea leaves extract in phenylhydrazine induced-anemia in albino rats

Onyeabo, Chimaraoke; Achi, Ngozi; Ekeleme-Egedigwe, Chima A.; Ebere, Chidinma U; Okoro, Chidinma K

doi:10.17306/j.afs.0492

Cited by 22 publications

(21 citation statements)

References 25 publications

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“…The result revealed that the plant extract is rich in these bioactive compounds. This has confirmed the reports on phytochemical screening of Justicia carnea by other authors who also reported high contents of these bioactive compounds [24,25]. Phenolic compounds constitute the major group of compounds that take part in the antioxidant activity of natural products such as fruits, vegetables and all plant materials [26].…”

Section: Discussionsupporting

confidence: 88%

In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

Chidi

Ani

Kelechi

et al. 2020

IJBcRR

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This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

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Section: Discussionsupporting

confidence: 88%

In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

Chidi

Ani

Kelechi

et al. 2020

IJBcRR

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“…Justicia carnea is a flowering plant that belongs to Acanthaceae family. The genus Justicia was named after a Scottish Gardner James Justice, in the 18 th century [9]. It is popularly used in eastern Nigeria as blood tonic prepared by decoction [9].…”

Section: Introductionmentioning

confidence: 99%

“…The genus Justicia was named after a Scottish Gardner James Justice, in the 18 th century [9]. It is popularly used in eastern Nigeria as blood tonic prepared by decoction [9]. Several species of Justicia are traditionally used in the management of several ailments such as: inflammation, gastrointestinal disorders, diarrhoea, liver diseases, rheumatism and arthritis [10,11].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effect of Methanol Extract of Justicia carnea Leaves on Carbon Tetrachloride-Intoxicated Albino Rats

Jc¹,

Pac²,

Ag³

2019

Biochem Anal Biochem

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Introduction: Hepatotoxicity can be caused by drugs overdose (in some cases even when the drugs are administered in therapeutic doses). This may damage the liver temporarily or permanently. The reason for this study is to evaluate the amount of damage caused by carbon tetrachloride (CCl 4 ) on liver and establish a dose-dependent treatment and prevention with Justicia carnea leaves extract.Aim: Hepatoprotective effect of methanol extract of Justicia carnea leaves on carbon tetrachloride intoxicated albino rats was investigated. Methods:A total of thirty-five female wistar albino rats were used for the study and were grouped into 7 groups (I-VII) of 5 rats each. Group I served as normal control which received only feed and water, group II served as CCl 4treated control receiving 0.5 mg/kg bodyweight (bw) in of CCl 4 alone in olive oil (1:1 v/v), group III served as extract control which received 1000 mg/kg bw of methanol extract of Justicia carnea (JC) only, groups IV, V and VI received 200, 500 and 1000 mg/kg bw of methanol extract of Justician carnea respectively prior to CCl 4 administration while group VII received 100 mg/kg bw of silymarin as a standard drug prior to CCl 4 administration. Some biochemical parameters were evaluated using biochemical methods. Results:The activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and bilirubin levels showed a significant (p<0.05) increase in the group treated with CCl 4 only when compared with the normal control while total protein and albumin levels showed a significant (p<0.05) decrease in the group treated with CCl 4 only when compared with the normal control. However, groups pre-treated with different doses of 200, 500 and 1000 mg/kg bw of Justicia carnea showed a significant (p<0.05) decrease in the activities of ALT, AST and ALP, and bilirubin level while total protein and albumin levels increased significantly (p<0.05) when compared with the rats treated with CCl 4 alone. Histopathological examinations of sections of the liver collected from the different groups showed that the normal control had a normal liver architecture compared to the group treated with CCl 4 alone which showed a diffuse vacuolar degeneration and coagulative necrosis of the hepatocytes. However, groups pre-treated with different doses of the extract showed lesser necrosis and a better liver architecture compared with the group treated with CCl 4 alone. Conclusion:The results of this study suggest that the methanol extract of Justicia carnea may exhibit hepatoprotective properties that may be effective in the management of liver toxicity.

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“…The above conditions were reversed after the administration of silymarin and CaL-extracts to rats in groups 3 and 4-6 respectively. This reversal indicates that CaL-extract could exhibit some anti-anaemic properties with the phytochemicals, antioxidants and vitamins present [35]. The increase in PCV after the administration of CaL-extract, could be attributed to a possible induction of hematopoietic mechanism in response to the low PCV level resulting in the production of more blood cells.…”

Section: Discussionmentioning

confidence: 92%

Potential of Centella asiatica leaf extract in the management of paracetamol-induced jaundice in albino rats

Uchechi¹,

Uchenna²,

Precious³

et al. 2020

World J. Adv. Res. Rev.

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Jaundice is a condition associated with the imbalance between bilirubin production and excretion when the liver is diseased. This study investigated the Potential of Centella asiatica leaf extract in the management of paracetamolinduced jaundice in albino rats. The rats were randomly distributed into six groups of five each. Group 1 (normal control-fed with rat feed and water), group 2 (paracetamol 2 g/kg body weight), group 3 (paracetamol 2g/kg bw + silymarin 100 mg/kg bw) and groups 4 -6 (2g/kg bw each + 200, 400 and 600 mg/kg bw of CaL-extract respectively). The Paracetamol was first administered to the rats for 5 days to establish jaundice followed by administration of silymarin and CaL-extract for 10 days before sacrifice. The group 2 (negative control) rats had significant increase (p<0.05) in glucose level (7.50±0.10 mmol/L) and haematological parameters like haemoglobin (8.65±0.15g/dL), packed cell volume (26.15±0.95%), red blood cell (3.75±0.05x10 12 /L) and platelet (248.00±1.00 x10 9 /L) as well as elevated levels of serum lipids (LDL, TG,TC VLDL-cholesterol) when compared with group 3 and the CaL-treated groups. Results of the liver function maker enzymes (AST, ALT and ALP) activity, serum bilirubin and proteins as well as renal function indices (creatinine, urea, chloride and bicarbonate) in group 2 were significantly increased (p<0.05); while endogenous antioxidant study, revealed low levels of catalase (1.24±0.02µmol/ml), superoxide dismutase (0.17±0.02unit/ml) and reduced glutathione (0.84±0.18mg/ml) when compared to the treated groups. Administration of the CaL-extract and silymarin ameliorated the paracetamol-induced jaundice in albino rats.

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Haematological and biochemical studies on Justicia carnea leaves extract in phenylhydrazine induced-anemia in albino rats

Cited by 22 publications

References 25 publications

In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

Hepatoprotective Effect of Methanol Extract of Justicia carnea Leaves on Carbon Tetrachloride-Intoxicated Albino Rats

Potential of Centella asiatica leaf extract in the management of paracetamol-induced jaundice in albino rats

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