The biochemical implication of chloramphenicol-induced lymphoma and the ameliorative potential of Justicia carnea and Cnidoscolus aconitifolius on male Wistar rats were evaluated in this study. Seventy (70) male Wistar rats with average body weights of 128g were randomly grouped into 14 of 5 rats each. Group 1 received commercial rat feed and water ad libitum. Rats in groups 2-14 were given 250mg/kg bodyweight of chloramphenicol by oral intubation for 28 days. Results of blood samples collected after 28 days from all the groups showed normal blood film in Group 1 and abnormal increases and lymphocytes and the presence of blast cells in Groups 2-14. Group 2 did not receive any treatment and is referred to as the negative control. The remaining groups (3-14) were administered with aqueous leaf extracts of J. carnea (Groups 3-6), C. aconitifolius (Groups 7-10), and a combination of both extracts (Groups 11-14) in doses of 500mg/kg, 1000mg/kg, 1500mg/kg and 2000mg/kg respectively, for 28 days. Data obtained showed that lymphocytes were elevated (p<0.05) in Group 2 lymphocytosis and there was the presence of blast cells indicating lymphoma when compared to Group 1. The combination of J. carnea and C. aconitifolius was able to ameliorate the chloramphenicol-induced lymphoma better than the single therapy of each extract. The result of the investigation supports the earlier findings that chloramphenicol could cause acute lymphocytic leukemia. It further provides evidence that combined extracts of J. carnea and C. aconitifolius may have an ameliorative effect in blood diseases connected to over-exposure to chloramphenicol.

This study aimed to evaluate the In vitro digestibilities, predicted glycemic index and sensory evaluation of biscuits produced from composite flours of wheat and processed tiger nut. The tiger nut was sorted and processed by air-drying, blanching, fermentation and dehydration.The processed samples were oven dried at a temperature of 60 o C for 17 hours before milling into fine flour to blend with wheat flour (Wf) at the ratio of 20:80. Biscuits were produced from blends of wheat flour and processed tiger nut namely: wheat-tiger nut air dried (W-TNA), wheat-tiger nut blanched (W-TNB), wheat-tiger nut fermented (W-TNF) and wheat-tiger nut dehydrated (W-TND). The results of In-vitro protein digestibility of the composite biscuit showed W-TNF biscuit to have the highest digestibility (52.53%) and W-TND as the least (39.44%) when compared to wheat flour (48.18%). Highest starch digestibilities were recorded for composite biscuits from Wf (99.45±2.32%) and W-TNF (89.99±1.67%). The highest hydrolysis index (HI) of 138.85%, and 113.79% were observed in W-TNF and W-TND respectively; which also resulted in higher predicted glycemic index (PGI) of 115.93% (W-TNF) and 102.17% (W-TND).The sensory evaluation revealed that the composite biscuits were generally accepted although there were reductions in aroma, texture and taste of the composite biscuits when compared with the control (Wf)

Jaundice is a condition associated with the imbalance between bilirubin production and excretion when the liver is diseased. This study investigated the Potential of Centella asiatica leaf extract in the management of paracetamolinduced jaundice in albino rats. The rats were randomly distributed into six groups of five each. Group 1 (normal control-fed with rat feed and water), group 2 (paracetamol 2 g/kg body weight), group 3 (paracetamol 2g/kg bw + silymarin 100 mg/kg bw) and groups 4 -6 (2g/kg bw each + 200, 400 and 600 mg/kg bw of CaL-extract respectively). The Paracetamol was first administered to the rats for 5 days to establish jaundice followed by administration of silymarin and CaL-extract for 10 days before sacrifice. The group 2 (negative control) rats had significant increase (p<0.05) in glucose level (7.50±0.10 mmol/L) and haematological parameters like haemoglobin (8.65±0.15g/dL), packed cell volume (26.15±0.95%), red blood cell (3.75±0.05x10 12 /L) and platelet (248.00±1.00 x10 9 /L) as well as elevated levels of serum lipids (LDL, TG,TC VLDL-cholesterol) when compared with group 3 and the CaL-treated groups. Results of the liver function maker enzymes (AST, ALT and ALP) activity, serum bilirubin and proteins as well as renal function indices (creatinine, urea, chloride and bicarbonate) in group 2 were significantly increased (p<0.05); while endogenous antioxidant study, revealed low levels of catalase (1.24±0.02µmol/ml), superoxide dismutase (0.17±0.02unit/ml) and reduced glutathione (0.84±0.18mg/ml) when compared to the treated groups. Administration of the CaL-extract and silymarin ameliorated the paracetamol-induced jaundice in albino rats.

No abstract

No abstract