Haematological and biochemical profile of harvest-size carp during harvest and post-harvest storage

Abstract

Stress load on harvest‐size common carp (Cyprinus carpio L.) just before harvest, during harvest and during the post‐harvest storage were assessed using haematological and biochemical indices. The study was carried out at the 60 ha Dřemliny pond for 28, 7 and 1 day before harvest, during 3‐day harvest and after a 28‐day post‐harvest period. Indices monitored included the relative spleen weight (SSI), erythrocyte count (Er), haemoglobin levels (Hb), haematocrit (PCV), MCV, MCH, MCHC, leucocrit (Bc), differential leucocyte count (leucogram), blood plasma cortisol, glucose, total proteins (TP), cholesterol and chloride. During harvest, a significant decrease of SSI (P<0.05; P<0.01), increase of PCV (P<0.01), decrease of Bc (P<0.01, P<0.05), lymphopenia (P<0.05) and neutrophilia (P<0.01) were observed. A significant increase in the levels of blood plasma cortisol was found 1 day before harvest (P<0.05) due to draining off the pond before harvest itself, hyperglycaemia was observed 1 day before harvest (P<0.05) and during harvest (P<0.01); a decrease, on the other hand, was found in cholesterol and chloride levels (P<0.01). The relationships between oxygen content in water and SSI and MCHC were positive (P>0.05) and between oxygen and glucose were negative (P>0.01). A 28‐day post‐harvest storage returned the indices investigated back to physiological interval of values. Persistently lower levels of Bc, lymphopenia, neutrophilia and lower TP concentrations are indicative of deepening immunosuppression during the post‐harvest storage period. Changes of haematological and biochemical indices during harvest in relation to hypoxia, fish handling and higher stocking rates are also discussed.

Selected Haematological and Biochemical Indices of Nile Tilapia (Oreochromis niloticus) Reared in the Environment with Cyanobacterial Water Bloom

“…The decrease of cholesterol levels may indicate slight damage of the hepatopancreas, similar to the significant increase of lactate levels. The negative effect of different pollutants at sublethal concentrations and stress of fish may be indicated by a decrease in cholesterol values (Gluth and Hanke 1985;Svobodova et al 2006). However, no decrease of cholesterol was found in our study; on the contrary, we noticed an increase.…”

Modulation of Biochemical and Haematological Indices of Silver Carp (Hypophthalmichthys molitrix Val.) Exposed to Toxic Cyanobacterial Water Bloom

“…The negative effect of different pollutants at sublethal concentrations and stress of fish may be indicated by a decrease in cholesterol values (Gluth and Hanke 1985;Svobodová et al 2006). The decrease in cholesterol concentrations in our study may indicate a slight injury of the hepatopancreas.…”

“…Bury et al (1996) and Ernst et al (2006) observed slightly increased glucose concentrations in fish (brown trout and whitefish, respectively) exposed to cyanobacteria, but changes were not significant. An increase in glucose is a general response of fish to acute pollutant effects and stress (Lusková et al 2002;Svobodová et al 2006). Consequently, stress as indicated by plasma glucose changes does not appear to be associated with liver pathology.…”

Physiological responses of carp (Cyprinus carpio L.) to dietary exposure to zearalenone (ZEN)

“…The present study provides more evidence of organ-wide effects of ZEN in carp and reports a large variety of biochemical serum parameters which are known to be affected by many endogenous and exogenous factors including age, health condition, nutrition or stress, including chemical stress (Svobodova et al, 2006;Hoseini and Ghelichpour, 2013). Total protein content in serum of all carp used in the present study was slightly lower in most cases (Table 3) compared with previously reported values in other studies (Hoseini and Ghelichpour, 2013;Varga et al, 2013;Pietsch et al, 2014b).…”