H9, H10, and H11 compose a cluster of Hessian fly-resistance genes in the distal gene-rich region of wheat chromosome 1AS

Abstract

H9, H10, and H11 are major dominant resistance genes in wheat, expressing antibiosis against Hessian fly [(Hf) Mayetiola destructor (Say)] larvae. Previously, H9 and H10 were assigned to chromosome 5A and H11 to 1A. The objectives of this study were to identify simple-sequence-repeat (SSR) markers for fine mapping of these genes and for marker-assisted selection in wheat breeding. Contrary to previous results, H9 and H10 did not show linkage with SSR markers on chromosome 5A. Instead, H9, H10, and H11 are linked with SSR markers on the short arm of chromosome 1A. Both H9 and H10 are tightly linked to flanking markers Xbarc263 and Xcfa2153 within a genetic distance of 0.3-0.5 cM. H11 is tightly linked to flanking markers Xcfa2153 and Xbarc263 at genetic distances of 0.3 cM and 1.7 cM. Deletion bin mapping assigned these markers and genes to the distal 14% of chromosome arm 1AS, where another Hf-resistance gene, Hdic (derived from emmer wheat), was also mapped previously. Marker polymorphism results indicated that a small terminal segment of chromosome 1AS containing H9 or H10 was transferred from the donor parent to the wheat lines Iris or Joy, and a small intercalary fragment carrying H11 was transferred from the resistant donor to the wheat line Karen. Our results suggest that H9, H10, H11, Hdic, and the previously identified H9- or H11-linked genes (H3, H5, H6, H12, H14, H15, H16, H17, H19, H28, and H29) may compose a cluster (or family) of Hf-resistance genes in the distal gene-rich region of wheat chromosome 1AS; and H10 most likely is the same gene as H9.

Identification of a novel gene, H34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers

“…One HFR QTL was detected on the chromosome 1A of 'Clark', designated as Qhf-hwwg-1A, and very closely linked to Xgwm33, a marker closely linked to 1A gene cluster of 15 HFR genes (Stebbins et al 1983;Roberts and Gallun 1984;Liu et al 2005aLiu et al , 2005bKong et al 2005Kong et al , 2008. This QTL is likely H6 derived from 'Caldwell' (Ratcliffe et al, 2000;Chen et al 2009) and appears to contribute a minor effect (accounting for about 10% of the phenotypic variance) to resistance to GP biotype in this study.…”

“…In this study, we used a RIL population instead of F 2 as reported in most previous studies (Dweikat et al 2002;Martín-Sánchez et al 2003;Kong et al 2005Kong et al , 2008Liu et al 2005bLiu et al , 2005cYu et al 2009) to improve phenotyping accuracy. RILs have a high recombination frequency resulting from multiple meiotic events that occurred during repeated selfing (Jansen 2003), and a high level of homozygosity that enables replicated phenotyping across different environments.…”

“…This QTL is likely H6 derived from 'Caldwell' (Ratcliffe et al, 2000;Chen et al 2009) and appears to contribute a minor effect (accounting for about 10% of the phenotypic variance) to resistance to GP biotype in this study. To date, many molecular markers, including SSR, randomly amplified polymorphic DNA, and sequence tag sites, for HFR genes in 1AS cluster have been published (Dweikat et al 1997(Dweikat et al , 2002Liu et al 2005aLiu et al , 2005bKong et al 2005Kong et al , 2008Bouktila et al 2006). SNP markers that are suitable for high-throughput screening have not been reported in the 1AS cluster.…”

Resistance to Wheat Curl Mite in Arthropod-Resistant Rye-Wheat Translocation Lines

“…Interactions between resistant plants and avirulent arthropods involve incompatible arthropod-plant interactions mediated by constitutively produced and arthropod-induced plant defense proteins synthesized by resistance gene products [9]. Several genes from barley, rye, wheat and wild wheat relatives provide functional resistance against A. toschiella, D. noxia or M. destructor [10][11][12][13][14][15][16][17]. However, the evolution of virulence in each pest causes continual delays in the development of additional new resistant cultivars [18,19].…”

“…Over thirty genes from wheat and its relatives convey M. destructor resistance [17]. However, only plants containing H13, H18, H21, H25, H26, and Hdic are consistently effective against M. destructor populations in the Great Plains wheat production area of Texas, Oklahoma, and Kansas [25].…”