H3K36 demethylase JMJ710 negatively regulates drought tolerance by suppressing MYB48-1 expression in rice

Abstract

The homeostasis of histone methylation is maintained by histone methyltransferases and demethylases, which are important for the regulation of gene expression. Here, we report a histone demethylase from rice (Oryza Sativa), Jumonji C (jmjC) domain containing protein (JMJ710), which belongs to the JMJD6 group and plays an important role in the response to drought stress. Overexpression of JMJ710 causes a drought-sensitive phenotype, while RNAi and CRISPR-knockout mutant lines show drought tolerance. In vitro and in vivo assays showed that JMJ710 is a histone demethylase. It targets to MYB TRANSCRIPTION FACTOR 48 (MYB48-1) chromatin, demethylates H3K36me2, and negatively regulates the expression of MYB48-1, a positive regulator of drought tolerance. Under drought stress, JMJ710 is downregulated and the expression of MYB48-1 increases, and the subsequent activation of its downstream drought-responsive genes leads to drought tolerance. This research reports a negative regulator of drought stress-responsive genes, JMJ710, that ensures that the drought tolerance mechanism is not mis-activated under normal conditions but allows quick activation upon drought stress.

Grapevine bZIP transcription factor bZIP45 regulates VvANN1 and confers drought tolerance in Arabidopsis

“…Approximately 1 g of plant tissue was harvested from two-week-old transgenic hybrid progeny seedlings that were germinated on MS plates in a light incubator. Samples were prepared according to previous reports ( Yamaguchi et al., 2014 ; Zhao et al., 2022 ). ChIP experiments were performed using Abcam ChIP Kit - Plants (ab117137, Abcam, Cambridge, MA, USA) according to the manufacturer’s instructions.…”

Methylation hallmarks on the histone tail as a linker of osmotic stress and gene transcription

“…In non-model plants, there is mounting evidence of histone demethylation involved in osmotic stress. OsJMJ710 targets to MYB48-1 and demethylates H3K36me2, and dowregulates the expression of MYB48-1 , leading to repressed drought tolerance in rice ( Zhao et al, 2022 ). OsJMJ703 negatively regulates both the floral development and the drought tolerance.…”

SNAC1‐OsERF103‐OsSDG705 module mediates drought response in rice

“…These processes collectively dictate crucial facets of gene functions, encompassing expression levels, protein activity, and stability (Mann & Jensen, 2003; Hunter, 2007; Deribe et al ., 2010; Friso & van Wijk, 2015; Vu et al ., 2018; Lee et al ., 2023). Histone modifications emerge as pivotal players of post‐translational modifications, intricately woven into the fabric of plant development and their responses to environmental cues (Mirouze & Paszkowski, 2011; Zhao et al ., 2022). The methylation patterns on histones, particularly histone 3 lysine 9 (H3K9), H3K27, and H4K20, are associated with transcriptional repression, while methylation on H3K4 and H3K36 is correlated with gene activation (Sui et al ., 2012; Freitag, 2017), in which H3K4me3 has been reported to be involved in heat acclimation, floral transition, dehydration stress‐responding memory, development leaf senescence and so on (N. Liu et al ., 2014; Brusslan et al ., 2015; Tan et al ., 2020; Zong et al ., 2020; Yamaguchi & Ito, 2021).…”

“…The methylation patterns on histones, particularly histone 3 lysine 9 (H3K9), H3K27, and H4K20, are associated with transcriptional repression, while methylation on H3K4 and H3K36 is correlated with gene activation (Sui et al ., 2012; Freitag, 2017), in which H3K4me3 has been reported to be involved in heat acclimation, floral transition, dehydration stress‐responding memory, development leaf senescence and so on (N. Liu et al ., 2014; Brusslan et al ., 2015; Tan et al ., 2020; Zong et al ., 2020; Yamaguchi & Ito, 2021). Although a wealth of literature has documented the direct involvement of various regulators in the transcriptional control of drought responses, a mounting body of evidence is now underscoring the predominant role played by epigenetic regulation in the context of drought stress (Yamaguchi‐Shinozaki & Shinozaki, 2006; Ding et al ., 2012; Kim et al ., 2017; Huang et al ., 2019; Chang et al ., 2020; K. Chen et al ., 2021; Zhao et al ., 2022). Furthermore, the intricate interplay between transcriptional regulators and the dynamic landscape of epigenetic modifications during stress response remains largely elusive in rice.…”

“…Although a wealth of literature has documented the direct involvement of various regulators in the transcriptional control of drought responses, a mounting body of evidence is now underscoring the predominant role played by epigenetic regulation in the context of drought stress (Yamaguchi-Shinozaki & Shinozaki, 2006;Ding et al, 2012;Kim et al, 2017;Huang et al, 2019;Chang et al, 2020;K. Chen et al, 2021;Zhao et al, 2022). Furthermore, the intricate interplay between transcriptional regulators and the dynamic landscape of epigenetic modifications during stress response remains largely elusive in rice.…”