H2S is involved in ABA-mediated stomatal movement through MPK4 to alleviate drought stress in Arabidopsis thaliana

The coordination of guard-cell autonomous ABA synthesis and DES1 function in situ regulates plant water deficit responses

“…This result suggests that ABA3-mediated-guard cell ABA synthesis is sufficient to trigger dehydration induced- DES1 expression. DES1 is involved in stomatal closure and related drought tolerance responses [15] , [18] , [20] . To further evaluate the possible link between the functions of ABA3 and DES1 in drought stress responses, DES1 was specifically expressed in the guard cell of aba3 mutant under control of guard cell-specific MYB60 promoter, to produce pMYB60:DES1 aba3 lines.…”

“…Hydrogen sulfide (H 2 S), which has long been considered only as a toxic gas, has been known in recent years to be an important gaseous signaling molecule that regulates plant growth, development and stress responses [13] , [14] , [15] , [16] . Pretreatment with exogenous NaHS (a H 2 S donor) alleviates drought stress responses by increasing ABA synthesis through the expression of ABA synthesis genes in wheat [17] .…”

Hydrogen sulfide, a signaling molecule in plant stress responses

“…Hydrogen sulfide mediates multiple ion fluxes that induce stomatal closure under drought conditions in Arabidopsis (Scuffi et al, 2018). In addition, H 2 S interacts with a variety of molecules to assist in the activation of the outflow ion channel and enhance the inhibition of the inflow ion channel (Jin et al, 2013; Du et al, 2019; Chen et al, 2020b), thus, reducing the osmotic potential and the expansion rate of the cell (Figure 2). Currents associated with S‐type anion channels are activated by low concentrations of exogenously applied H 2 S in wildtype Arabidopsis , but not in guard cells of the open stomata 1 ( ost1) mutant (Wang et al, 2016).…”

Abscisic acid‐triggered guard cell l‐cysteine desulfhydrase function and in situ hydrogen sulfide production contributes to heme oxygenase‐modulated stomatal closure

“…By exogenously using its donor, H 2 S was reported to confer positive effects in multiple physiological processes, such as root organogenesis (Zhang et al, 2009), plant responses to phytohormones (Hou et al, 2013;Scuffi et al, 2014), and tolerance toward drought, salt, oxidative, and heavy-metal stresses (Jin et al, 2013(Jin et al, , 2017Zhang et al, 2009). Several independent groups have reported the participation of H 2 S in ABA-and ethylene-dependent stomatal closure induction (Du et al, 2019;García-Mata & Lamattina, 2010;Hou et al, 2013;Jin et al, 2013). It has long been noted that higher plants have L-Cys desulfhydrase (DES) activity with the release of H 2 S, pyruvate, and ammonia during its catalyzed L-Cys degradation (Harrington & Smith, 1980;Papenbrock, Riemenschneider, Kamp, Schulz-Vogt, & Schmidt, 2007).…”

“…Although the null mutants of DES1 showed an approximately 30% decrease in total DES activity, it was clearly demonstrated that DES1 expression was hormonally regulated. Importantly, DES1-produced H 2 S acts early in ABA-dependent stomatal closure and interacts with other second messengers or protein kinases to relay the guard cell signaling network, such as NO, ROS, and MAP kinase as well (Corpas, González-Gordo, Cañas, & Palma, 2019;Du et al, 2019;Scuffi et al, 2014Scuffi et al, , 2018.…”