Gypsum fibrosum and its major component CaSO4 increase cutaneous aquaporin-3 expression levels

Ikarashi, Nobutomo; Ogiue, Naoki; Toyoda, Eri; Kon, Risako; Ishii, Makoto; Toda, Takahiro; Aburada, Takashi; Ochiai, Wataru; Sugiyama, Kiyoshi

doi:10.1016/j.jep.2011.11.025

Cited by 23 publications

(13 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study, we showed that Gypsum fibrosum increased the concentration of cutaneous Ca 6) In addition, it was reported that CaCl 2 increased the expression level of AQP3 in keratinocytes.…”

Section: Discussionmentioning

confidence: 85%

“…38) In our previous study, we demonstrated that Gypsum fibrosum increased the expression level of cutaneous AQP3 in mice. 6) In this study, the mechanism of the Gypsum fibrosum-induced increase in cutaneous AQP3 expression was investigated using a human keratinocyte cell line.…”

Section: Discussionmentioning

confidence: 99%

“…5) In addition, it has been reported that the CaSO 4 in Gypsum fibrosum can lead to an increase in cutaneous AQP3 expression levels by triggering an increase in the concentration of cutaneous Ca. 6) In this study, we elucidated the mechanism by which Gypsum fibrosum leads to an increase in cutaneous AQP3, focusing on the increase in the intracellular concentration of cutaneous Ca. The mechanism by which the expression of cutaneous AQP3 increases following the addition of Gypsum fibrosum was evaluated using the human papillomavirus type 16-immortalized human keratinocyte cell line (PHK16-0b).…”

Section: Elucidating the Mechanism By Whichmentioning

confidence: 99%

See 2 more Smart Citations

Elucidating the Mechanism by Which <i>Gypsum fibrosum</i>, a Traditional Chinese Medicine, Maintains Cutaneous Water Content

Ikarashi

Ogiue

Toyoda

et al. 2013

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

Aquaporin-3 (AQP3) plays an important role in maintaining the normal water content of the skin. Previously, we revealed that the expression of cutaneous AQP3 increased following oral administration of Gypsum fibrosum (main component: CaSO 4 ) to mice. The purpose of this study is to elucidate the mechanism by which Gypsum fibrosum increases the expression of cutaneous AQP3 in a keratinocyte cell line. Gypsum fibrosum or CaSO 4 was added to keratinocytes, and the expression level of AQP3, the Ca concentration, the activity of protein kinase C (PKC), and the degrees of phosphorylation of both extracellular signal-regulated kinase (ERK) and cAMP response element binding protein (CREB) were measured. The mRNA and protein expression levels of AQP3 increased significantly 6 h-post addition of Gypsum fibrosum. In keratinocytes treated with Gypsum fibrosum, increases in the concentration of intracellular Ca, PKC activity, and the phosphorylation of ERK and CREB were observed. Pre-treatment with GF109203X, a PKC inhibitor, suppressed the mRNA expression levels of AQP3. Similarly to treatment with Gypsum fibrosum, the addition of CaSO 4 led to the same observations in keratinocytes. It is hypothesized that Gypsum fibrosum causes an increase in the intracellular Ca concentration, PKC activity, and the phosphorylation levels of ERK and CREB, resulting in increased AQP3 expression in keratinocytes. In addition, it is possible that the effect of Gypsum fibrosum is attributable to CaSO 4 , based on the results demonstrating that the mechanisms of action of Gypsum fibrosum and CaSO 4 were nearly identical.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Elucidating the Mechanism By Whichmentioning

confidence: 99%

See 1 more Smart Citation

Elucidating the Mechanism by Which <i>Gypsum fibrosum</i>, a Traditional Chinese Medicine, Maintains Cutaneous Water Content

Ikarashi

Ogiue

Toyoda

et al. 2013

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Temperature elevation in mice treated with ephedrine has been shown to be suppressed by GF treatment , and GF improves thirst observed in STZ‐induced diabetic mice . Accordingly, we focused on GF in BKN by investigating the effects of GF on the expression of AQP2 in kidney and AQP3 in skin, and compared the activity of GF with that of CaSO 4 .…”

Section: Gypsum Fibrosum and Increased Aqp Expression In Kidney And Skinmentioning

confidence: 99%

“…We formulated and attempted to verify our hypothesis that the efficacy of BKN is exerted by controlling the expression of AQP. This review provides an overview of findings regarding the water control mechanisms of BKN and its activity with regard to AQP, with a focus on our studies .…”

Section: Introductionmentioning

confidence: 99%

Water control mechanism of byakkokaninjinto and its active components via aquaporins

Ikarashi

Aburada

Kon

et al. 2019

Traditional & Kampo Medicine

Self Cite

View full text Add to dashboard Cite

Objective: Byakkokaninjinto (BKN) is clinically used for conditions associated with thirst, polyuria, or cutaneous pruritus.Recently, it has also been used for improving thirst and polyuria observed in diabetes as well as pruritus associated with excessively dry skin. The aim of this study was to investigate aquaporins (AQP) in order to obtain scientific evidence regarding the mechanisms of BKN's effect. Results: Treatment with BKN in a diabetic model of KKAy mice did not affect blood glucose but reduced both water intake and urine volume. Although the AQP5 level in the salivary glands remained unchanged, the AQP2 level in the kidneys increased significantly. This suggests that BKN causes an increase in AQP2 expression in the kidney, thereby suppressing polyuria as well as the associated thirst caused by diabetes. BKN was also shown to increase the expression of AQP3 in skin, which may suppress skin dryness and improve pruritus. In addition, Gypsum fibrosum (GF), the principal component of BKN, did not affect kidney AQP2 expression but increased skin AQP3 expression. The active ingredient of GF, CaSO 4 , was also shown to increase AQP3 expression via protein kinase C activation and to enhance phosphorylation of cAMP response element-binding protein by increasing the Ca 2+ concentration in skin. Conclusion: Although there are many herbal medicines that are effective for correcting abnormal water metabolism, little is known regarding the mechanism of this activity. This study highlights the scientific basis of the effect of herbal medicines in improving water metabolism, with AQP as the primary targets.

show abstract

Aquaporins in the Skin

Patel

Heard

Chen

et al. 2017

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

The skin is the largest organ of the body, serving as an important barrier between the internal milieu and the external environment. The skin is also one of the first lines of defense against microbial infection and other hazards, and thus, the skin has important immune functions . This organ is composed of many cell types, including immune-active dendritic cells (epidermal Langerhans cells and dermal dendritic cells), connective tissue-generating dermal fibroblasts and pigment-producing melanocytes. Comprising the outer skin layer are the epidermal keratinocytes, the predominant cell of this layer, the epidermis , which provides both a mechanical barrier and a water -permeability barrier. Recent data suggest that aquaporins, a family of barrel-shaped proteins surrounding internal pores that allow the passage of water and, in some family members, small solutes such as glycerol , play critical roles in regulating various skin parameters. The involvement of different aquaporin family members in skin function is discussed.

show abstract

Gypsum fibrosum and its major component CaSO4 increase cutaneous aquaporin-3 expression levels

Cited by 23 publications

References 26 publications

Elucidating the Mechanism by Which <i>Gypsum fibrosum</i>, a Traditional Chinese Medicine, Maintains Cutaneous Water Content

Elucidating the Mechanism by Which <i>Gypsum fibrosum</i>, a Traditional Chinese Medicine, Maintains Cutaneous Water Content

Water control mechanism of byakkokaninjinto and its active components via aquaporins

Aquaporins in the Skin

Contact Info

Product

Resources

About