Gynogenesis Induction in Sugar Beet (Beta vulgaris) Improved by 6-Benzylaminopurine (BAP) and Synergized with Cold Pretreatment

Pazuki, Arman; Aflaki, Fatemeh; Gürel, Ekrem; Ergül, Ali; Gürel, Songül

doi:10.1007/s12355-017-0522-x

Cited by 25 publications

(28 citation statements)

References 32 publications

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“…Methods of producing gynogenic plant material had been explained previously (Pazuki et al 2017a); here, the methods are described briefly. Inflorescences from a diploid self-fertile sugar beet (Beta vulgaris) genotype (i.e.…”

Section: Plant Materials and Gynogenesismentioning

confidence: 99%

“…As described previously (Pazuki et al 2017a), fresh leaf tissue (1.5 cm 2 ) of in vitro cultured gynogenic sugar beet and fresh leaf tissue (1 cm 2 ) of common vetch (Vicia sativa) (2C = 3.65 pg) were chopped up simultaneously with a sharp razor blade in 400 µL of extraction buffer of CyStain UV precise P (Partec, Münster, Germany). The suspended nuclei were incubated for 30 s and then the suspension was passed through a CellTrics® 30 µm filter into a test tube.…”

Section: Nuclear Dna Measurementmentioning

confidence: 99%

“…Sugar beet explants are very sensitive to hyperhydricity while growing in vitro (Pazuki et al 2017a). The problem can arise from an excess of the ammonium ion (NH 4 + ) (Debergh et al 1981), cytokinins (CKs) (Ivanova and van Staden 2011), high water potential or relative humidity of the in vitro medium (Debergh et al 1981, ethylene production by explants (Kevers and Gaspar 1985), and stress-induced changes in the physiological state of explants (Kevers et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…6-benzylaminopurine (BAP) is one of the growth regulators from among the CKs, which has been widely used in sugar beet ovule culture for gynogenesis (reviewed in Aflaki et al 2017). However, the proliferated or regenerated plantlets can be hyperhydric (Tomita et al 2013, Pazuki et al 2017a. A hyperhydric sugar beet subculture on a medium with a new composition or under new conditions takes time and effort to produce a normal plant, which is a costly practice (Tomaszewska-Sowa 2012).…”

Section: Introductionmentioning

confidence: 99%

“…A hyperhydric sugar beet subculture on a medium with a new composition or under new conditions takes time and effort to produce a normal plant, which is a costly practice (Tomaszewska-Sowa 2012). The superior effect of kinetin (Kin) over BAP has been observed in sugar beet gynogenesis (Pazuki et al 2017a(Pazuki et al , 2017b. It was recorded that BAP could cause higher hyperhydricity than Kin.…”

Section: Introductionmentioning

confidence: 99%

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A robust method for haploid sugar beet in vitro proliferation and hyperhydricity reduction

Pazuki

Aflaki

Gürel

et al. 2017

Folia Horticulturae

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Sugar beet is recalcitrant to in vitro tissue culture. Usually, proliferation of in vitro cultured rosette explants is a prerequisite for micropropagation. Although hormonal treatments can induce proliferation in sugar beet rosette explants, they may also result in some side effects. In vitro culture of sugar beet explants and some hormonal treatments make them more prone to hyperhydricity. Effects of media with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin) on the proliferation and hyperhydricity of haploid sugar beet explants were investigated. It was observed that 0.2 mg L -1 Kin, with a reasonable amount of proliferation and minimum rate of hyperhydricity, performed better than BAP in different concentrations and combinations. The effect sizes of the treatments on the dependent variables were large. The correlation between proliferation and hyperhydricity of the treated explants was statistically negative and the association was large. However, the hormonal treatments without BAP or with the lowest amount of it produced the highest proliferation rate with the least hyperhydricity. The coefficient of determination was R 2 quadratic = 0.885. The results suggest that, in comparison with BAP, Kin is a potent plant growth regulator for the proliferation of sugar beet haploid explants that causes the least hyperhydricity. Although explants proliferated better in the presence of 0.01 mg L -1 BAP in combination with Kin than under Kin alone, the hyperhydricity of the proliferated explants decreased their suitability for in vitro propagation.

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Section: Plant Materials and Gynogenesismentioning

confidence: 99%

Section: Nuclear Dna Measurementmentioning

confidence: 99%