Gut microbiota signature as predictors of adverse outcomes after acute ischemic stroke in patients with hyperlipidemia

Abstract

IntroductionThe alterations of gut microbiota have been associated with multiple diseases. However, the relationship between gut microbiota and adverse outcomes of hyperlipidemic stroke patients remains unclear. Here we determined the gut microbial signature to predict the poor outcome of acute ischemic stroke (AIS) with hyperlipidemia (POAH).MethodsFecal samples from hyperlipidemic stroke patients were collected, which further analyzed by 16s rRNA gene sequencing. The diversity, community composition and differential gut microbiota were evaluated. The adverse outcomes were determined by modified Rankin Scale (mRS) scores at 3 months after admission. The diagnostic performance of microbial characteristics in predicting adverse outcomes was assessed by receiver operating characteristic (ROC) curves.ResultsOur results showed that the composition and structure of gut microbiota between POAH patients and good outcome of AIS with hyperlipidemia (GOAH) patients were different. The characteristic gut microbiota of POAH patients was that the relative abundance of Enterococcaceae and Enterococcus were increased, while the relative abundance of Lachnospiraceae, Faecalibacterium, Rothia and Butyricicoccus were decreased. Moreover, the characteristic gut microbiota were correlated with many clinical parameters, such as National Institutes of Health Stroke Scale (NIHSS) score, mean arterial pressure, and history of cerebrovascular disease. Moreover, the ROC models based on the characteristic microbiota or the combination of characteristic microbiota with independent risk factors could distinguish POAH patients and GOAH patients (area under curve is 0.694 and 0.971 respectively).ConclusionsThese findings revealed the microbial characteristics of POAH, which highlighted the predictive capability of characteristic microbiota in POAH patients.

Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients

“…At present, there are some studies focusing on the gut microbiota of AIS or specific AIS populations, such as the study reported by Chen et al. ( Chen et al., 2022 ). Chen’s study was to investigate the characteristic gut microbiota of poor outcome of AIS with hyperlipidemia (POAH) patients.…”

“…Stool samples (200 mg) were collected and stored in the Fecal Microbial Collection and Preservation Kit (ML-001A, Shenzhen Dayun Gene Technology Co., Ltd.) and saved in an -80°C refrigerator within 3 days ( Chen et al., 2022 ; Luo et al., 2022 ). Total DNA was extracted following the manufacturer’s instructions using CTAB method.…”

Protective effects of fecal microbiota transplantation against ischemic stroke and other neurological disorders: an update

“…Some studies have reported that GM composition influences stroke prognosis ( 57 , 58 ). The GBA regulates immunological functions in the post-stroke condition while altered GM composition and abundance affect post-stroke outcomes mainly via gut leakiness, endotoxemia, and the neuroimmune axis.…”

The role of gut microbiota in cerebrovascular disease and related dementia

“…These findings demonstrate that GM might be an important regulator of the prognosis of hyperlipidaemic stroke and its consequences. In this sense, a recent study demonstrated that the GM signature of hyperlipidaemic patients is a predictor of adverse outcomes after acute ischaemic stroke, as determined by modified Rankin Scale (mRS) scores at 3 months after admission (Chen, Chi, et al, 2022). Hypertension is the most prevalent modifiable risk factor for stroke and dementia (van der Flier et al, 2018).…”