Gut microbiota role in dietary protein metabolism and health-related outcomes: The two sides of the coin

Portune, Kevin J.; Beaumont, Martín; Davila, Anne‐Marie; Tomé, Daniel; Blachier, François; Sanz, Yolanda

doi:10.1016/j.tifs.2016.08.011

Cited by 260 publications

(250 citation statements)

References 197 publications

Supporting

Mentioning

227

Contrasting

Unclassified

Order By: Relevance

“…In a preliminary experiment, we found that free dietary tryptophan supplementation (0.5% w/v in drinking water) in mice failed to increase indole production, as evaluated by excretion of I3S in the urine, a commonly used proxy for intestinal production of indole (55, 56). Because free amino acids are highly absorbed in the small intestine, they might not reach the distal part of the gut, where the bacterial density is higher (17). In contrast, some dietary proteins escape the small intestine (<10%) (57) and might be an important source of exogenous tryptophan for the microbiota (17).…”

Section: Discussionmentioning

confidence: 99%

“…Because free amino acids are highly absorbed in the small intestine, they might not reach the distal part of the gut, where the bacterial density is higher (17). In contrast, some dietary proteins escape the small intestine (<10%) (57) and might be an important source of exogenous tryptophan for the microbiota (17). Indeed, in humans, high-protein diets increase indole production by the microbiota, as shown by an increase in I3S urinary excretion (58).…”

Section: Discussionmentioning

confidence: 99%

“…One of the most striking findings of these studies is that the liver is exposed to microbial metabolites produced from aromatic amino acids (AAA) such as phenylacetate, benzoate, p -cresol, and indole (17). Indeed, the concentrations of the corresponding host–microbiota cometabolites ( i.e.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The gut microbiota metabolite indole alleviates liver inflammation in mice

et al. 2018

Self Cite

View full text Add to dashboard Cite

The gut microbiota regulates key hepatic functions, notably through the production of bacterial metabolites that are transported via the portal circulation. We evaluated the effects of metabolites produced by the gut microbiota from aromatic amino acids (phenylacetate, benzoate, p-cresol, and indole) on liver inflammation induced by bacterial endotoxin. Precision-cut liver slices prepared from control mice, Kupffer cell (KC)-depleted mice, and obese mice (ob/ob) were treated with or without LPS and bacterial metabolites. We observed beneficial effects of indole that dose-dependently reduced the LPS-induced up-regulation of proinflammatory mediators at both mRNA and protein levels in precision-cut liver slices prepared from control or ob/ob mice. KC depletion partly prevented the antiinflammatory effects of indole, notably through a reduction of nucleotide-binding domain and leucine-rich repeat containing (NLR) family pyrin domain-containing 3 (NLRP3) pathway activation. In vivo, the oral administration of indole before an LPS injection reduced the expression of key proteins of the NF-κB pathway and downstream proinflammatory gene up-regulation. Indole also prevented LPS-induced alterations of cholesterol metabolism through a transcriptional regulation associated with increased 4β-hydroxycholesterol hepatic levels. In summary, indole appears as a bacterial metabolite produced from tryptophan that is able to counteract the detrimental effects of LPS in the liver. Indole could be a new target to develop innovative strategies to decrease hepatic inflammation.—Beaumont, M., Neyrinck, A. M., Olivares, M., Rodriguez, J., de Rocca Serra, A., Roumain, M., Bindels, L. B., Cani, P. D., Evenepoel, P., Muccioli, G. G., Demoulin, J.-B., Delzenne, N. M. The gut microbiota metabolite indole alleviates liver inflammation in mice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The gut microbiota metabolite indole alleviates liver inflammation in mice

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…Proteomic, genomic, and metabolomics studies have recently observed the ability of oxidized amino acids to modulate gene expression, leading to impaired physiological functions (Li, Shi, Le, Ding, & Zhao, ; Yang, Zhang et al., ; Yang, Yan et al., ,). Finally, animal proteins, heme iron and derived protein oxidation products may alter the microbiome, making the intestinal mucosa more sensitive to both biological and chemical threats (Constante, Fragoso, Calvé, Samba‐Mondonga, & Santos, ; Portune et al., ; Van der Leek, Yanishevsky, & Kozyrskyj, ). As follows, the most recent scientific evidences of the role of dietary oxidized proteins on particular pathological disorders are reported.…”

Section: Pathogenesis Of Dietary Protein Oxidation: Recent Advancesmentioning

confidence: 99%

Intake of Oxidized Proteins and Amino Acids and Causative Oxidative Stress and Disease: Recent Scientific Evidences and Hypotheses

Estévez

Xiong

2019

Journal of Food Science

113

View full text Add to dashboard Cite

The exposure to reactive oxygen species (ROS) is an inevitable consequence of living in an aerobic world. The species contribute to the occurrence of oxidative stress in humans in which an uncontrolled production of ROS exceeds the endogenous antioxidant defences leading to the oxidative damage to essential cellular components, such as lipids, proteins, and DNA. The influence of diet on the modulation of the systemic redox status is recognized and, while some dietary components are found to be protective (that is, fruits and vegetables), others are recognized as pro‐oxidants (that is, processed meat and other animal‐source protein foods). Oxidized proteins and amino acids are potential promoters of luminal and postprandial oxidative stress; preliminary studies have actually reported noxious effects of these species in cultured cells and in experimental animals. However, the underlying pathological mechanisms remain poorly understood. The application of advanced methodological approaches based on mass spectrometric technologies and OMICS disciplines has enabled the elucidation of the molecular basis of the pathological effects of dietary oxidized proteins and amino acids. The present review collects the most recent evidences of the health risks of dietary protein oxidation and proposes reasonable hypotheses and future perspectives on the field.

show abstract

“…A wide variety of bacterial metabolites can be produced from peptides and AA reaching the colon (for review, see [29]), some amino acid precursors and their main products being presented in Table 1. In humans, HP diets induce a global increase in AA degradation by the microbiota probably through an increase in protein supply and protease activity in the lumen of the large intestine [25,27,30].…”

Section: Potential Influence Of Dietary Proteins On Inflammatory Bmentioning

confidence: 99%

Dietary Protein and Amino Acid Supplementation in Inflammatory Bowel Disease Course: What Impact on the Colonic Mucosa?

et al. 2017

Self Cite

View full text Add to dashboard Cite

Inflammatory bowel diseases (IBD), after disease onset, typically progress in two cyclically repeated phases, namely inflammatory flare and remission, with possible nutritional status impairment. Some evidence, either from epidemiological, clinical, and experimental studies indicate that the quantity and the quality of dietary protein consumption and amino acid supplementation may differently influence the IBD course according to the disease phases. For instance, although the dietary protein needs for mucosal healing after an inflammatory episode remain undetermined, there is evidence that amino acids derived from dietary proteins display beneficial effects on this process, serving as building blocks for macromolecule synthesis in the wounded mucosal area, energy substrates, and/or precursors of bioactive metabolites. However, an excessive amount of dietary proteins may result in an increased intestinal production of potentially deleterious bacterial metabolites. This could possibly affect epithelial repair as several of these bacterial metabolites are known to inhibit colonic epithelial cell respiration, cell proliferation, and/or to affect barrier function. In this review, we present the available evidence about the impact of the amount of dietary proteins and supplementary amino acids on IBD onset and progression, with a focus on the effects reported in the colon.

show abstract

Gut microbiota role in dietary protein metabolism and health-related outcomes: The two sides of the coin

Cited by 260 publications

References 197 publications

The gut microbiota metabolite indole alleviates liver inflammation in mice

The gut microbiota metabolite indole alleviates liver inflammation in mice

Intake of Oxidized Proteins and Amino Acids and Causative Oxidative Stress and Disease: Recent Scientific Evidences and Hypotheses

Dietary Protein and Amino Acid Supplementation in Inflammatory Bowel Disease Course: What Impact on the Colonic Mucosa?

Contact Info

Product

Resources

About