Gut microbiota modulation and anti-inflammatory properties of mixed lactobacilli in dextran sodium sulfate-induced colitis in mice

Abstract

Lactobacillus acidophilus KDSL 1.0901，Lactobacillus helveticus KDSL 1.8701and Lactobacillus plantarum KDSL 1.0318 showed antibacterial activity, antioxidant activity and immunomodulatory activity. In the current study, we evaluated the effects of three tested...

Lactobacillus plantarum HNU082 alleviates dextran sulfate sodium-induced ulcerative colitis in mice through regulating gut microbiome

“…38 IL-6 promotes inflammation by regulating STAT3 signaling to activate NF-κB. 14 MPO, as a product of neutrophil infiltration, is proportional to the degree of inflammation. 39 Furthermore, loss of IL-10 spontaneously causes IBD, while TGF-β1 and TGF-β2 can significantly alleviate DSS-induced intestinal inflammation.…”

“…After natural clotting of the blood for 30 min, the serum was separated and collected by centrifugation at 3000 rpm for 20 min at 4 °C. 13 The levels of interleukin (IL)-1β, IL-6, IL-10, and IL-17A, interferon (IFN)-γ, tumour necrosis factor-alpha (TNF-α) and myeloperoxidase (MPO) were measured in the mouse colon using appropriate ELISA kits (X-Y Biotechnology, Shanghai, China), as described previously. 14…”

“…13 The levels of interleukin (IL)-1β, IL-6, IL-10, and IL-17A, interferon (IFN)-γ, tumour necrosis factor-alpha (TNF-α) and myeloperoxidase (MPO) were measured in the mouse colon using appropriate ELISA kits (X-Y Biotechnology, Shanghai, China), as described previously. 14…”

Oral administration of octacosanol modulates the gut bacteria and protects the intestinal barrier in ulcerative colitis mice

“…The tightness and integrity of the gut barrier are crucial in preventing the development of IBD (Shi et al, 2021). Lipopolysaccharide…”

“…The tightness and integrity of the gut barrier are crucial in preventing the development of IBD (Shi et al, 2021). Lipopolysaccharide (LPS), the major component of the gram‐negative bacteria's outer membrane, can decrease the expression of TJs and indicate gut permeability (Wang et al, 2021).…”