Gut microbiota differently contributes to intestinal immune phenotype and systemic autoimmune progression in female and male lupus-prone mice

Johnson, Benjamin M.; Gaudreau, Marie-Claude; Gudi, Radhika; Brown, Robert R.; Gilkeson, Gary S.; Vasu, Chenthamarakshan

doi:10.1101/787440

Cited by 9 publications

(51 citation statements)

References 60 publications

(129 reference statements)

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“…Gut microbial composition were analyzed using fresh fecal samples collected at weeks 0, 4, and 7 of the experimental period (Figure 1). Individually, none of the NOR, HE, and HO groups showed intra‐group similarity in the fecal bacterial distribution patterns among these observation periods (Figure S4A) and were consistent with the previous reports 32‐34 . Moreover, Chao 1 analysis and the observed OTUs from 16S rRNA sequencing data revealed that the alpha diversity of the gut microbial community did not differ significantly among the aforementioned experimental groups at any measurement time points (weeks 0, 4, and 7; Figure S2A).…”

Section: Resultssupporting

confidence: 90%

“…Individually, neither the SHAM nor the SHO group showed any intra‐group similarity in the fecal bacterial distribution patterns among weeks 0, 4, and 7 of the experimental period (Figure S4B), in agreement with the previous studies 32‐34 . Furthermore Chao‐1 analysis and the OTUs observed from 16S rRNA sequencing data showed that the alpha diversity of gut microbial communities did not differ significantly between SHAM and SHO groups at all time‐points of measurements (weeks 0, 4, and 7; Figure S2B).…”

Section: Resultssupporting

confidence: 90%

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Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models

Shin

Bose²,

Wang

et al. 2020

FASEB j.

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Thyroid hormones are essential for the regulation of energy homeostasis and metabolic processes. However, the relationship between thyroid function and host gut microbial communities is not properly understood. To determine whether and how gut microbiota is associated with thyroid function, metagenomics analysis of the bacterial population in fecal samples of rat models of hyperthyroidism (induced by levothyroxine) and hypothyroidism (induced by propylthiouracil or thyroidectomy) was conducted through 16S rRNA gene sequencing. Our results revealed that all thyroid dysfunction models were definitely established and gut microbial composition varied according to different thyroid functional status. The relative abundance of Ruminococcus was significantly higher in the hyperthyroidism group (HE) vs both the normal and hypothyroidism groups (HO) while S24‐7 was significantly higher in the HO group. The population of Prevotellaceae and Prevotella were significantly lower in the HO group vs the normal. Firmicutes and Oscillospira were significantly higher in the SHO (surgery‐induced hypothyroidism) group, while Prevotellaceae and Prevotella showed lower abundance in the SHO group than the SHAM group. Present results suggest that thyroid functions may have the potential to influence the profile of gut microbiota and could be used as foundation to investigate interaction mechanism between thyroid and gut microbiome.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 90%

Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models

Shin

Bose²,

Wang

et al. 2020

FASEB j.

View full text Add to dashboard Cite

show abstract

“…Importantly, anti-DNA antibodies of IgA class are found in the serum of patients with SLE 24 – 29 , suggesting that they may be of gut primed B cell origin. These reports along with our studies 16 , 17 showing pro-inflammatory immune phenotype and higher plasma cell frequency by lupus-prone female mouse intestine suggests the degree of IgA secretion in the gut lumen could show gender bias and may be indicative of lupus susceptibility and autoimmune progression. Nevertheless, the relationship between fecal IgA levels and gender bias in lupus is unknown.…”

Section: Introductionsupporting

confidence: 70%

“…We have demonstrated that minor dietary deviations alter the composition of gut microbiota and SLE in a mouse model 13 . We have also found that gut microbiota influences the autoimmune progression differently in lupus-prone male and female mice, leading to a gender bias in disease incidence 16 .…”

Section: Introductionmentioning

confidence: 67%

“…Our recent studies that used lupus-prone (SWRxNZB)F1 (SNF1) mice showed a potential contribution of pro-inflammatory immune response initiated in the gut mucosa, and gut microbiota in triggering the disease associated gender bias observed in SLE 16 , 17 . We also showed that pro-inflammatory responses including higher cytokine expression, recruitment of large number of immune cells, and presence of higher number of antibody positive plasma cells in the gut mucosa of lupus-prone females, compared to males, can be detected as early as at juvenile age.…”

Section: Introductionmentioning

confidence: 99%

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Abundance and nuclear antigen reactivity of intestinal and fecal Immunoglobulin A in lupus-prone mice at younger ages correlate with the onset of eventual systemic autoimmunity

Sun

Gudi

Johnson

et al. 2020

Sci Rep

Self Cite

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Our recent studies, using (SWRxNZB)F1 (SNF1) mice, showed a potential contribution of the gut microbiota and pro-inflammatory immune responses of the gut mucosa to systemic autoimmunity in lupus. Here, using this mouse model, we determined the abundance and the nAg reactivity of IgA antibody produced in the intestine under lupus susceptibility. Intestinal lymphoid tissues from SNF1 mice, females particularly, showed significantly higher frequencies of nAg (dsDNA and nucleohistone) reactive IgA producing B cells compared to B6 females. Most importantly, younger age fecal IgA -abundance and -nAg reactivity of lupus-prone mice showed a positive correlation with eventual systemic autoimmunity and proteinuria onset. Depletion of gut microbiota in SNF1 mice resulted in the diminished production of IgA in the intestine and the nAg reactivity of these antibodies. Overall, these observations show that fecal IgA features, nuclear antigen reactivity particularly, at preclinical stages/in at-risk subjects could be predictive of autoimmune progression.

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Negative correlation between IL‐1β, IL‐12 and TNF‐γ, and cortisol levels in patients with panic disorder

et al. 2022

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Introduction Chronic exposure to stress is a major risk factor in anxiety disorders (ADs) and can be accompanied by an altered microbiome–gut–brain axis and a compromised immune system. In recent years, the study of inflammatory processes in AD has gained special attention. Continued stress causes the reactivity of the hypothalamic–pituitary–adrenal (HPA) axis, the alteration of the intestinal microbiota and the consequent release of pro‐inflammatory cytokines, affecting the sensitivity to stress and the similar behavior of anxiety. Method The aim of the present study was to evaluate the interrelationships between measures of proinflammatory cytokines and cortisol in patients with panic disorder (PD). Results The main results of the correlation analysis revealed that the levels of pro‐inflammatory cytokines interleukin (IL)‐1β, IL‐12, and tumor necrosis factor gamma were negatively correlated with cortisol scores (area under the curve with respect to the ground). Conclusions These results suggest that the inflammatory response is associated with the reactivity of the HPA axis in patients with PD and may influence the maintenance of anxiety behavior.

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Gut microbiota differently contributes to intestinal immune phenotype and systemic autoimmune progression in female and male lupus-prone mice

Cited by 9 publications

References 60 publications

Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models

Chemically or surgically induced thyroid dysfunction altered gut microbiota in rat models

Abundance and nuclear antigen reactivity of intestinal and fecal Immunoglobulin A in lupus-prone mice at younger ages correlate with the onset of eventual systemic autoimmunity

Negative correlation between IL‐1β, IL‐12 and TNF‐γ, and cortisol levels in patients with panic disorder

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