Gut dysbiosis in cystic fibrosis

Scanlan, Pauline D.; Buckling, Angus; Kong, Weidong; Wild, Yvette K.; Lynch, Susan V.; Harrison, Freya

doi:10.1016/j.jcf.2012.03.007

Cited by 57 publications

(44 citation statements)

References 9 publications

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“…The diversity of the gut microbiota of individuals with CF was also significantly reduced compared to that of healthy controls. This is a common finding of studies on CF gut microbiota compared to controls [39, 40]. Indeed, it appears from studies on children with CF that this decrease in diversity occurs early in life for CF patients, with diversity decreasing with age, with CF teenagers having diversity levels similar to those seen in healthy control infants (1 year) [39].…”

Section: Discussionmentioning

confidence: 88%

The altered gut microbiota in adults with cystic fibrosis

Burke¹,

Fouhy²,

Harrison³

et al. 2017

BMC Microbiol

124

156

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BackgroundCystic Fibrosis (CF) is an autosomal recessive disease that affects the function of a number of organs, principally the lungs, but also the gastrointestinal tract. The manifestations of cystic fibrosis transmembrane conductance regulator (CFTR) dysfunction in the gastrointestinal tract, as well as frequent antibiotic exposure, undoubtedly disrupts the gut microbiota. To analyse the effects of CF and its management on the microbiome, we compared the gut microbiota of 43 individuals with CF during a period of stability, to that of 69 non-CF controls using 454-pyrosequencing of the 16S rRNA gene. The impact of clinical parameters, including antibiotic therapy, on the results was also assessed.ResultsThe CF-associated microbiome had reduced microbial diversity, an increase in Firmicutes and a reduction in Bacteroidetes compared to the non-CF controls. While the greatest number of differences in taxonomic abundances of the intestinal microbiota was observed between individuals with CF and the healthy controls, gut microbiota differences were also reported between people with CF when grouped by clinical parameters including % predicted FEV1 (measure of lung dysfunction) and the number of intravenous (IV) antibiotic courses in the previous 12 months. Notably, CF individuals presenting with severe lung dysfunction (% predicted FEV1 ≤ 40%) had significantly (p < 0.05) reduced gut microbiota diversity relative to those presenting with mild or moderate dysfunction. A significant negative correlation (−0.383, Simpson’s Diversity Index) was also observed between the number of IV antibiotic courses and gut microbiota diversity.ConclusionsThis is one of the largest single-centre studies on gut microbiota in stable adults with CF and demonstrates the significantly altered gut microbiota, including reduced microbial diversity seen in CF patients compared to healthy controls. The data show the impact that CF and it's management have on gut microbiota, presenting the opportunity to develop CF specific probiotics to minimise microbiota alterations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-0968-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 88%

The altered gut microbiota in adults with cystic fibrosis

Burke¹,

Fouhy²,

Harrison³

et al. 2017

BMC Microbiol

124

156

View full text Add to dashboard Cite

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“…are consistent with previous findings (Scanlan et al. 2012; Duytschaever et al. 2013; Schippa et al.…”

Section: Resultsmentioning

confidence: 99%

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

Miragoli

Federici

Ferrari

et al. 2016

FEMS Microbiology Ecology

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Cystic fibrosis is often associated with intestinal inflammation due to several factors, including altered gut microbiota composition. In this study, we analyzed the fecal microbiota among patients with cystic fibrosis of 10–22 years of age, and compared the findings with age-matched healthy subjects. The participating patients included 14 homozygotes and 14 heterozygotes with the delF508 mutation, and 2 heterozygotes presenting non-delF508 mutations. We used PCR-DGGE and qPCR to analyze the presence of bacteria, archaea and sulfate-reducing bacteria. Overall, our findings confirmed disruption of the cystic fibrosis gut microbiota. Principal component analysis of the qPCR data revealed no differences between homozygotes and heterozygotes, while both groups were distinct from healthy subjects who showed higher biodiversity. Archaea were under the detection limit in all homozygotes subjects, whereas methanogens were detected in 62% of both cystic fibrosis heterozygotes and healthy subjects. Our qPCR results revealed a low frequency of sulfate-reducing bacteria in the homozygote (13%) and heterozygote (13%) patients with cystic fibrosis compared with healthy subjects (87.5%). This is a pioneer study showing that patients with cystic fibrosis exhibit significant reduction of H2-consuming microorganisms, which could increase hydrogen accumulation in the colon and the expulsion of this gas through non-microbial routes.

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“…Microbial imbalance in the human skin microbiome [1,2] has been termed “skin dysbiosis” [3]. Here we refer to the precision microbiome as an approach for normalization of dysbiotic microbiome by selectively targeting specific microbes.…”

Section: Introductionmentioning

confidence: 99%

“…Propionibacterium acnes ( P. acnes ) and Staphylococcus epidermidis ( S. epidermidis ) are two major bacterial inhabitants of lesions in acne vulgaris [2]. Although both P. acnes and S. epidermidis are human skin commensals, the over-growth of P. acnes has been recognized for its association with the progression of acne vulgaris [1,3]. The biological role of S. epidermidis within acne lesions remains mysterious.…”

Section: Introductionmentioning

confidence: 99%

A Precision Microbiome Approach Using Sucrose for Selective Augmentation of Staphylococcus epidermidis Fermentation against Propionibacterium acnes

Wang

Kao

et al. 2016

IJMS

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Acne dysbiosis happens when there is a microbial imbalance of the over-growth of Propionibacterium acnes (P. acnes) in the acne microbiome. In our previous study, we demonstrated that Staphylococcus epidermidis (S. epidermidis, a probiotic skin bacterium) can exploit glycerol fermentation to produce short-chain fatty acids (SCFAs) which have antimicrobial activities to suppress the growth of P. acnes. Unlike glycerol, sucrose is chosen here as a selective fermentation initiator (SFI) that can specifically intensify the fermentation activity of S. epidermidis, but not P. acnes. A co-culture of P. acnes and fermenting S. epidermidis in the presence of sucrose significantly led to a reduction in the growth of P. acnes. The reduction was abolished when P. acnes was co-cultured with non-fermenting S. epidermidis. Results from nuclear magnetic resonance (NMR) analysis revealed four SCFAs (acetic acid, butyric acid, lactic acid, and succinic acid) were detectable in the media of S. epidermidis sucrose fermentation. To validate the interference of S. epidermidis sucrose fermentation with P. acnes, mouse ears were injected with both P. acnes and S. epidermidis plus sucrose or phosphate buffered saline (PBS). The level of macrophage-inflammatory protein-2 (MIP-2) and the number of P. acnes in ears injected with two bacteria plus sucrose were considerably lower than those in ears injected with two bacteria plus PBS. Our results demonstrate a precision microbiome approach by using sucrose as a SFI for S. epidermidis, holding future potential as a novel modality to equilibrate dysbiotic acne.

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Gut dysbiosis in cystic fibrosis

Cited by 57 publications

References 9 publications

The altered gut microbiota in adults with cystic fibrosis

The altered gut microbiota in adults with cystic fibrosis

Impact of cystic fibrosis disease on archaea and bacteria composition of gut microbiota

A Precision Microbiome Approach Using Sucrose for Selective Augmentation of Staphylococcus epidermidis Fermentation against Propionibacterium acnes

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