Gut Bacterial Diversity of Insecticide-Susceptible and -Resistant Nymphs of the Brown Planthopper Nilaparvata lugens Stå l (Hemiptera:  Delphacidae) and Elucidation of Their Putative Functional Roles

Vijayakumar, Malathi M.; More, Ravi Prabhakar; Anandham, Rangasamy; Gracy, R. Gandhi; Mohan, M.; Venkatesan, T.; Samaddar, Sandipan; Jalali, S. K.; Sa, Tongmin

doi:10.4014/jmb.1711.11039

Cited by 23 publications

(12 citation statements)

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“…Our results show that the Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were the dominant phyla in SBPH, and these four phyla are particularly strongly represented in insects (Douglas, 2015). Proteobacteria and Firmicutes were the two most predominant phyla and similar to the microbes previously studied in the brown planthopper, Nilaparvata lugens, another pest that sucks the phloem sap of rice plants (Vijayakumar et al, 2018;Zhang et al, 2019). It may be a similar feeding habit that leads to similarities in the microbiome of these two species of planthopper.…”

Section: Discussionsupporting

confidence: 79%

Antibiotic exposure perturbs the bacterial community in the small brown planthopper Laodelphax striatellus

Zhang

Zhou

et al. 2019

Insect Science

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Bacteria symbionts in herbivores play an important role in host biology and ecology, and are affected by environmental factors such as temperature, diet, habitat, antibiotics and so on. However, the effects of antibiotics on the microbiome of the small brown planthopper Laodelphax striatellus (SBPH) remain unclear. Here, we studied the effects of tetracycline on the diversity and composition of bacterial colonies in different tissues of SBPH using high throughput sequencing of 16S ribosomal RNA amplicons. Our results show that Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria were most abundant in SBPH, and the genera Asaia and Wolbachia were most abundant in all body parts of SBPH. Antibiotic treatment had persistent effects on the composition of the SBPH microbiome. Tetracycline depleted the population of Firmicutes, Bacteroidetes, Tenericutes and Fusobacteria, and nearly 100% eliminated Wolbachia, Bacteroides and Abiotrophia in SBPH. Together, these results suggest that antibiotic exposure affects the bacteria symbionts of different body parts in SBPH and will facilitate future studies of the bacterial symbionts of arthropod hosts.

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Section: Discussionsupporting

confidence: 79%

Antibiotic exposure perturbs the bacterial community in the small brown planthopper Laodelphax striatellus

Zhang

Zhou

et al. 2019

Insect Science

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“…A positive correlation between the number of insecticide‐related genes and Wolbachia density has been reported in mosquitoes 32,33 . Furthermore, higher bacterial abundance has been reported in an insecticide‐resistant population of the brown planthopper Nilaparvata lugens 34 . In our research, quantitative PCR was carried out to investigate the population dynamics of H. defensa in Hamiltonella ‐infected clones after treatment with various insecticides.…”

Section: Discussionmentioning

confidence: 74%

“…32,33 Furthermore, higher bacterial abundance has been reported in an insecticide-resistant population of the brown planthopper Nilaparvata lugens. 34 In our research, quantitative PCR was carried out to investigate the population dynamics of H. defensa in Hamiltonella-infected clones after treatment with various insecticides. Interestingly, H. defensa density was greatly decreased at 24 h but then increased until 72 h (Figure 3).…”

Section: Discussionmentioning

confidence: 99%

Reduced insecticide susceptibility of the wheat aphid Sitobion miscanthi after infection by the secondary bacterial symbiont Hamiltonella defensa

Sun

Qin

et al. 2020

Pest Management Science

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BACKGROUND Bacterial symbionts in insects, especially aphids, have a major influence on host adaptation. We previously showed that infection with the secondary symbiont Hamiltonella defensa increases the fitness of the wheat aphid Sitobion miscanthi, yielding increases in fitness parameters such as adult weight and offspring number. However, whether H. defensa affects the sensitivity of host aphids to insecticides remains unknown. RESULTS We tested the effects of H. defensa on host aphid susceptibility to the insecticides chlorpyrifos methyl, imidacloprid, cyantraniliprole and acetamiprid. Our results showed that compared with Hamiltonella‐free aphid clones, Hamiltonella‐infected aphid clones exhibited lower sensitivity to most of the tested insecticides at low concentrations. Quantitative polymerase chain reaction showed that the density of H. defensa in the infected clones was slightly decreased at 24 h but then sharply increased until the late stage after treatment with the different insecticides. H. defensa in the host aphids was detected by fluorescence in situ hybridization and was localized to the aphid hindgut. Levels of the detoxification enzymes acetylcholinesterase, glutathione transferase and carboxylesterase were significantly higher in Hamiltonella‐infected clones than in Hamiltonella‐free clones. CONCLUSIONS The findings indicated that infection with H. defensa reduced aphid susceptibility to the investigated insecticides at low concentrations, potentially by increasing detoxification enzyme activity in the host. Therefore, symbiont‐mediated insecticide resistance should be taken into account when performing resistance‐monitoring studies. Studies of symbiont‐mediated insecticide resistance may enhance our understanding of the emergence of insecticide resistance in agricultural systems. © 2020 Society of Chemical Industry

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“…In our study, bacteria from the orders Actinomycetales, Rhodospirillales, Erysipelotrichales, and Bacillales are significantly enriched in resistant populations. Likewise, Vijayakumar et al [ 83 ] reported that bacteria belonging to the orders Bacteroidales, Enterobacteriales, Clostridiales, Burkholderiales, Lactobacillales, and Bacillales were significantly more abundant in pesticide-resistant populations of the brown planthopper Nilaparvata lugens , one of the most important pests of rice [ 83 ]. In Plutella xylostella , a destructive pest of cruciferous crops, it was found that the order Lactobacillales (more abundant) and much scarcer taxa such as Pseudomonadales and Xanthomonadales were associated with the midgut and conferred resistance to chlorpyrifos and fipronil [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

The Midgut Microbiota of Colombian Aedes aegypti Populations with Different Levels of Resistance to the Insecticide Lambda-cyhalothrin

Arévalo-Cortés

Mejía-Jaramillo

Granada

et al. 2020

Insects

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Insecticide resistance in Aedes aegypti populations is a problem that hinders vector control and dengue prevention programs. In this study, we determined the susceptibility of Ae. aegypti populations from six Colombian regions to the pyrethroid lambda-cyhalothrin and evaluated the presence of the V1016I mutation in the sodium channel gene, which has been broadly involved in the resistance to this insecticide. The diversity of the gut microbiota of these mosquito populations was also analyzed. Only mosquitoes from Bello were susceptible to lambda-cyhalothrin and presented a lower allelic frequency of the V1016I mutation. Remarkably, there was not an important change in allelic frequencies among populations with different resistance ratios, indicating that other factors or mechanisms contributed to the resistant phenotype. Treatment of mosquitoes with antibiotics led us to hypothesize that the intestinal microbiota could contribute to the resistance to lambda-cyhalothrin. Beta diversity analysis showed significant differences in the species of bacteria present between susceptible and resistant populations. We identified 14 OTUs of bacteria that were unique in resistant mosquitoes. We propose that kdr mutations are important in the development of resistance to lambda-cyhalothrin at low insecticide concentrations but insect symbionts could play an essential role in the metabolization of pyrethroid insecticides at higher concentrations, contributing to the resistant phenotype in Ae. aegypti.

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Gut Bacterial Diversity of Insecticide-Susceptible and -Resistant Nymphs of the Brown Planthopper Nilaparvata lugens Stå l (Hemiptera: Delphacidae) and Elucidation of Their Putative Functional Roles

Cited by 23 publications

References 50 publications

Antibiotic exposure perturbs the bacterial community in the small brown planthopper Laodelphax striatellus

Antibiotic exposure perturbs the bacterial community in the small brown planthopper Laodelphax striatellus

Reduced insecticide susceptibility of the wheat aphid Sitobion miscanthi after infection by the secondary bacterial symbiont Hamiltonella defensa

The Midgut Microbiota of Colombian Aedes aegypti Populations with Different Levels of Resistance to the Insecticide Lambda-cyhalothrin

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