Gut bacterial ClpB-like gene function is associated with decreased body weight and a characteristic microbiota profile

Abstract

Background: The chaperone ClpB, a bacterial protein, is a conformational antigen-mimetic of α-melanocytestimulating hormone (α-MSH) implicated in body weight regulation in mice. We here investigated the potential associations of gut bacterial ClpB-like gene function with obesity status and gut microbiota in humans. Results: Gut microbiota ClpB KEGG function was negatively associated with body mass index, waist circumference, and total fat mass (DEXA). The relative abundance (RA) of several phyla and families directly associated with ClpB was decreased in subjects with obesity. Specifically, the RA of Rikenellaceae, Clostridiaceae and not assigned Firmicutes were lower in subjects with obesity and positively associated with gut bacterial ClpB-like gene function (not assigned Firmicutes (r = 0.405, FDR = 2.93 × 10−2), Rikenellaceae (r = 0.217, FDR = 0.031), and Clostridiaceae (r = 0.239, FDR = 0.017)). The gut bacterial ClpB-like gene function was also linked to specific plasma metabolites (hippuric acid and 3-indolepropionic acid) and fecal lupeol. The α-MSH-like epitope similar to that of Escherichia coli ClpB was also identified in some sequences of those bacterial families. After fecal transplantation from humans to mice, the families that more contributed to ClpB-like gene function in humans were also associated with ClpB-like gene function in mice after adjusting for the donor's body mass index (not assigned Firmicutes (r = 0.621, p = 0.003), Prevotellaceae (r = 0.725, p = 4.1 × 10 −7), Rikenellaceae (r = 0.702, p = 3.9 × 10 −4), and Ruminococcaceae (r = 0.526, p = 0.014)). Clostridiaceae (r = − 0.445, p = 0.038) and Prevotellaceae RA (r = − 0.479, p = 0.024) and were also negatively associated with weight gain in mice. The absolute abundance (AA) of Prevotellaceae in mice was also positively associated with the gut bacterial ClpB-like gene function in mice. DESeq2 identified species of Prevotellaceae, both negatively associated with mice' weight gain and positively with gut bacterial ClpB-like gene function. Conclusions: In summary, gut bacterial ClpB-like gene function is associated with obesity status, a specific gut microbiota composition and a plasma metabolomics profile in humans that could be partially transplanted to mice.

Effects of Microbiota Imbalance in Anxiety and Eating Disorders: Probiotics as Novel Therapeutic Approaches

“…The total SCFA were increased [ 75 , 76 ] and serotonin decreased after FMT [ 75 ]. On the other hand, Firmicutes , Rikenellaceae , Ruminococcaceae , Clostridiaceae, and Prevotellaceae are families of bacteria negatively associated with weight gain and positively associated with gut microbiota ClpB KEGG function (K03695, Kyoto Encyclopedia of Genes and Genomes annotation) after fecal transplantation from humans to mice [ 116 ]. Therefore, future studies to examine the role of fecal microbiota transplantation in eating behavior and weight gain may be helpful in unraveling altered pathways in EDs.…”

Cold Stress Activates the UCP3 Pathway via Gut Microbiota in Piglet

“…Muribaculaceae is newly named from family S24-7 (phylum Bacteroidetes) and includes versatile microorganisms involved in complex carbohydrate degradation and de ned as the main mucosal sugar utilizers to reduce Clostridiodes di cile colonization [37,38]. Rikenellaceae and Lachnospiraceae, which were increased after cold acclimation in pigs, could also have the potential ability to utilize mucosal sugars and may be associated with the host health [38][39][40][41]. Lachnospiraceae is also an important butyrate producer that impacts colonization resistance through antibiotic expression or intestinal acidi cation and in uence host mucosal immune cells and enterocytes [40].…”

Alterations of the Gut Microbiome and Metabolome in Patients with Proliferative Diabetic Retinopathy

“…Veillonellaceae are the key organisms in human gut that metabolize lactate [13], thereby reducing the risk of developing diabetic complication including PDR [14]. In addition, Clostridiales_unclassi ed, Ruminococcaceae, Firmicutes_unclassi ed, Clostridiaceae and Rikenellaceae were the TOP bacterial taxa at the family level contributing to the ClpB-like gene function that leads to reduced fat mass [15]. In contract to the bacteria with decreased abundance, the family Burkholderiaceae was the only bacterial taxa that enriched in the gut of PDR patients and the key discriminative microbial marker as identi ed by LEfSe analysis.…”