Gum arabic and red propolis protecteting colorectal preneoplastic lesions in a rat model of azoxymethane

Abstract

Purpose To evaluate red propolis, gum arabic and L-lysine activity on colorectal preneoplastic lesions induced by azoxymethane (AOM). Methods The study featured 4 control groups (I-IV) and 4 experimental groups (V-VIII), totaling 48 rats. Once a week for 2 weeks, animals on control groups received saline, while animals in experimental groups received azoxymethane (15 mg/kg i.p.). The follow up along 16 weeks included daily oral gavage to administer water (I and V), L-lysine (150 mg/kg)(II and VI), própolis (100mg/5ml/kg)(III and VII), or gum arabic (5ml/kg)(IV and VIII). Was performed surgery on the animals in the end of this time in order to collect blood for biological assays (TBARS, GSH), followed by their sacrifice to tissue extract. Results Oxidative stress (TBARS) and the number of aberrant crypt foci (ACF) in distal colon were lower using própolis (p<0.01 for both parameters). Gum arabic reduced preneoplastic lesions (ACF ≤ 4 crypts) on distal colon and on the entire colon (p<0.05). Conclusions Red propolis reduced AOM-induced oxidative stress (TBARS) and total number of ACF in the distal colon. L-lysine neither protected against nor enhanced AOM-induced ACF. Gum arabic reduced the number of ACF.

Bee Products and Colorectal Cancer—Active Components and Mechanism of Action

“…However, various doses of propolis combined with 5FU significantly reduced the number of ACFs compared to the treatment with 5FU alone, which indicated its synergistic effect with 5FU in mouse model Balb/c. The protective influence of propolis on the process of colon carcinogenesis in animal models was also observed by other authors [ 66 , 67 , 68 , 69 , 70 ]. A number of in vitro and in vivo studies confirmed the efficacy of the anti-cancer effects of propolis and encourage researchers to conduct clinical trials.…”

Red propolis reduces inflammation in cyclophosphamide-induced hemorrhagic cystitis in rats

“…All three yielded significant IC 50 values and performed better than the vitamin C standard (12). Also, our team tested the ability of red propolis treatment for 16 weeks to reduce oxidative stress based on TBARS quantification in peripheral blood of rats with azoxymethane-induced colon cancer (23). The development of the present experimental study was motivated by the results of in vitro and in vivo studies of the pathophysiology of CP-induced hemorrhagic cystitis and its association with oxidative stress (19).…”

Biomedical Properties of Propolis on Diverse Chronic Diseases and Its Potential Applications and Health Benefits

“…Reduces number of aberrant crypt foci in distal colon and TBARS levels in peripheral blood. [171] DMBA-induced oral squamous cell carcinomas in rats.…”

“…After 16 weeks, treatment with propolis (100 mg/mL) reduced the number of aberrant crypt foci in the distal colon. In addition, propolis reduced the TBARS (thiobarbituric acid reactive substances) levels in peripheral blood, suggesting a protective activity of Brazilian propolis in the colon, possibly associated with its antioxidant properties [ 171 ]. Similarly, the effect of oral administration of Brazilian red propolis (10, 50 and 100 mg/kg) for 26 weeks on oral squamous cell carcinomas (OSCC) in rats induced by 9,10-dimethyl-1,2-benzanthracene (DMBA) was investigated.…”