Guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear interpretation. Part I: control material

Jl, Vives Corrons; Albarède, Stéphanie; Flandrin, G; Heller, Silke; Horváth, Katalin; Houwen, B; Nordin, Gunnar; Sarkani, E; Skitek, Milan; M, Van Blerk; Jc, Libeer

doi:10.1515/cclm.2004.149

Cited by 31 publications

(22 citation statements)

References 2 publications

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“…Giemsa stain (10%) was prepared in Sorensen's buffer (pH 6.8) as described by Vives Corrons et al (35); the staining time was 20 minutes. For May-Grü nwald, the cells were first stained in May-Grü nwald solution for 3 to 5 minutes and subsequently with Giemsa (10%; see above) for 10 minutes (35).…”

Section: Methodsmentioning

confidence: 99%

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Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Nersesyan

Kundi

Atefie

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

165

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Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grü nwald-Giemsa) and DNA-specific (4 ¶,6-diamidino-2-phenylindole, Feulgen, acridine orange) stains, whereas with Giemsa-based stains, the frequencies of micronuclei in smokers were significantly (4-to 5-fold) higher in the smokers group, no significant increase was observed with any of the DNA-specific stains. Furthermore, the evaluation of cells of the two study groups with Feulgen stain showed that oral mucosa cells from smokers had significantly increased levels of nuclear anomalies other than micronuclei.These anomalies are consequences of cell injury found in epithelial cells and are paralleled by formation of keratin bodies in the cytoplasm that resemble micronuclei. Correlation analyses showed that micronuclei frequencies scored in Giemsa-stained slides correlated significantly with karyorrhexis, karyolysis, condensed chromatin, and binucleates, whereas no such correlations were found with DNA-specific stains. These findings indicate that nuclear anomalies (and possibly keratin bodies) may be misinterpreted as micronuclei with nonspecific DNA stains and lead to false-positive results in studies with cells of epithelial origin. Furthermore, our results show that exposure of oral mucosa cells to genotoxic carcinogens contained in tobacco smoke does not lead to induction of micronuclei in these cells. (Cancer Epidemiol Biomarkers Prev 2006;15(10):1835 -40)

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Section: Methodsmentioning

confidence: 99%

“…For May-Grü nwald, the cells were first stained in May-Grü nwald solution for 3 to 5 minutes and subsequently with Giemsa (10%; see above) for 10 minutes (35). Acridine orange was dissolved in bidistilled water (0.01%); the staining time was 15 minutes (36).…”

Section: Methodsmentioning

confidence: 99%

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Nersesyan

Kundi

Atefie

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

165

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“…In the literature, there are no guidelines describing how an external QA program for bone marrow assessment should be organized. There are, however, detailed guidelines related to peripheral blood smear examinations, which were prepared by the Hematology Working Group of the European External Committee for External Quality Assurance Programmes in Laboratory Medicine (EQALM) [5,6]. These guidelines were taken into account when preparing EQAhem, as one of the core tests of the EQAhem program is peripheral blood smear analysis.…”

Section: Discussionmentioning

confidence: 99%

External bone marrow cytological examination quality assurance (EQAhem)—summary after 6 years in Poland

2015

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Bone marrow macroscopic examination remains one of the most difficult and subjective laboratory assessments in hematology. Only a few external quality assurance programs in the field are present worldwide. We have developed an external quality assurance program EQAhem that allows assessment of the whole process of bone marrow examination. The program participants assess blood and bone marrow smears from the patient, identify selected cells from photographs provided to them, and interpret the microscopic results. In this article, the results of the EQAhem program in Poland from 6 years are summarized. During this time, 62 labs were assessed in total, and positive results were achieved by 89.25 % labs, taking into account all tests. Correct responses with respect to the percentage of cell count were provided by ca. 77.5 % labs. Slightly worse results were obtained when megakaryocyte count and cell identification from photographs were tested. The worst results were obtained in case of dysplasia assessment and clinical interpretation of microscopic examination (54.1 and 58.6 % correct responses, respectively). EQAhem delivers precise information about the quality of bone marrow examinations performed in Poland and has a substantial educational value. We believe that after 6 years, EQAhem has significantly improved the quality of bone marrow microscopic examinations performed in Poland.

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“…The smears of cells were prepared on pre-cleaned, pre-coded dried slides and Wxed in absolute methanol. The slides containing the cells were stained with 10% Giemsa (Sigma) prepared in Sorrenson's buVer, pH 5.6 according to the method of Vives Corrons et al [40] with minor modiWcations and examined under a microscope. 1,000 cells were counted blind for each animal.…”

Section: Genotoxicity Testingmentioning

confidence: 99%

Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract

Laikangbam

Devi

2011

Urol Res

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The present study aims at systematic evaluation of the calyces of Hibiscus sabdariffa to establish its scientific validity for anti-urolithiatic property using ethylene glycol-induced hyperoxaluria model in male albino rats. Administration of a mixture of 0.75% ethylene glycol and 2% ammonium chloride resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. The decrease in the serum calcium concentration indicates an increased calcium oxalate formation. Supplementation of aqueous extract of H. sabdariffa at different doses (250, 500 and 750 mg/kg body weight) significantly lowered the deposition of stone-forming constituents in the kidneys and serum of urolithiatic rats. These findings have been confirmed through histological investigations. Results of in vivo genotoxicity testing showed no significant chromosomal aberrations in the bone marrow cells of ethylene glycol-induced rats. The plant extracts at the doses investigated induced neither toxic nor lethal effects and are safe. It can be concluded that the calyces of H. sabdariffa are endowed with anti-urolithiatic activity and do not have genotoxic effects. Thus, it can be introduced in clinical practices and medicine in the form of orally administered syrup after further investigations and clinical trials.

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Guidelines for blood smear preparation and staining procedure for setting up an external quality assessment scheme for blood smear interpretation. Part I: control material

Cited by 31 publications

References 2 publications

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

External bone marrow cytological examination quality assurance (EQAhem)—summary after 6 years in Poland

Inhibition of calcium oxalate crystal deposition on kidneys of urolithiatic rats by Hibiscus sabdariffa L. extract

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