Guidelines for Assessing Mouse Endothelial Function via Ultrasound Imaging: a Report from the International Society of Cardiovascular Translational Research

Wang, Huating; Zhang, Shan; Li, Wen; Chu, Maoping; Yang, Jian; Yi, Dan; Zhan, Jiaxin; Yuan, Zuyi; Raikwar, Sudhanshu P.; Wang, Shenming; Zhang, Chunxiang

doi:10.1007/s12265-015-9614-8

Cited by 8 publications

(11 citation statements)

References 13 publications

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“…Atherosclerotic mice deficient in apolipoprotein E ( apoE −/− ) on a western diet exhibited impaired relaxation in vivo . These results matched gold-standard measurements in isolated carotid arterial rings ( Figures 3A–D ) ( 17 ). 11 C-acetate micro-PET imaging performed in mice exposed to hypercapnia stress was applied to evaluate systemic coronary vasodilatation and endothelial function in the heart and appeared instrumental to assess NO-mediated endothelial response ( 33 ).…”

Section: Multi-scale Imaging Of Heart and Large Vesselssupporting

confidence: 83%

“…In preclinical research, the acoustic response of targeted microbubbles is measured to visualize targeted molecular changes in CVD. These microbubbles allow for high sensitivity (10 −9 M) and accuracy (16)(17)(18)(19).…”

Section: Multi-scale Imaging Modalities In Vascular Imagingmentioning

confidence: 99%

“… Endothelial dysfunction imaging. (A,B) Ultrasound images of apoE −/− mouse carotid arteries at baseline and (C,D) after a dose of acetylcholine (20 μg/kg/min) on a Western diet [orange arrows represent the artery diameter; adopted from ( 17 )]. (E) Ex vivo fluorescence images of red fluorescent rhodaminebovine serum albumingadopentetate dimeglumine (rhodamine-BSA-GdDTPA) confined to the blood vessel in healthy myocardium and (F) corresponding three-dimensional (3D) visualization of permeability surface area product (PS) in healthy myocardium.…”

Section: Multi-scale Imaging Of Heart and Large Vesselsmentioning

confidence: 99%

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Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease

et al. 2022

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Cardiovascular disease entails systemic changes in the vasculature. The endothelial cells lining the blood vessels are crucial in the pathogenesis of cardiovascular disease. Healthy endothelial cells direct the blood flow to tissues as vasodilators and act as the systemic interface between the blood and tissues, supplying nutrients for vital organs, and regulating the smooth traffic of leukocytes into tissues. In cardiovascular diseases, when inflammation is sensed, endothelial cells adjust to the local or systemic inflammatory state. As the inflamed vasculature adjusts, changes in the endothelial cells lead to endothelial dysfunction, altered blood flow and permeability, expression of adhesion molecules, vessel wall inflammation, thrombosis, angiogenic processes, and extracellular matrix production at the endothelial cell level. Preclinical multi-scale imaging of these endothelial changes using optical, acoustic, nuclear, MRI, and multimodal techniques has progressed, due to technical advances and enhanced biological understanding on the interaction between immune and endothelial cells. While this review highlights biological processes that are related to changes in the cardiac vasculature during cardiovascular diseases, it also summarizes state-of-the-art vascular imaging techniques. The advantages and disadvantages of the different imaging techniques are highlighted, as well as their principles, methodologies, and preclinical and clinical applications with potential future directions. These multi-scale approaches of vascular imaging carry great potential to further expand our understanding of basic vascular biology, to enable early diagnosis of vascular changes and to provide sensitive diagnostic imaging techniques in the management of cardiovascular disease.

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Section: Multi-scale Imaging Of Heart and Large Vesselssupporting

confidence: 83%

Section: Multi-scale Imaging Modalities In Vascular Imagingmentioning

confidence: 99%

Section: Multi-scale Imaging Of Heart and Large Vesselsmentioning

confidence: 99%

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Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease

et al. 2022

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“…Isometric tension was measured in isolated mouse aortic ring segments as described [ 29 , 30 ]. The vessels were cut into individual ring segments (2–3 mm in width) and suspended from a force-displacement transducer in a tissue bath.…”

Section: Methodsmentioning

confidence: 99%

miR-34a is a common link in both HIV- and antiretroviral therapy-induced vascular aging

Zhan

Qin

Lü

et al. 2016

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Both HIV and antiretroviral therapy could induce vascular aging with unclear mechanisms. In this study, via microarray analysis, we identified, for the first time, that miR-34a expression was significantly increased in both HIV-infected, and antiretroviral agents-treated vessels and vascular endothelial cells (ECs) from these vessels. In cultured ECs, miR-34a expression was significantly increased by HIV-Tat protein and by the antiretroviral agents, lopinavir/ritonavir. Both HIV-Tat protein and antiretroviral agents could induce EC senescence, which was inhibited by miR-34a inhibition. In contrast, EC senescence was exacerbated by miR-34a overexpression. In addition, the vascular ECs isolated from miR-34a knockout mice were resistant to HIV and antiretroviral agents-mediated senescence. In vivo, miR-34a expression in mouse vascular walls and their ECs was increased by antiretroviral therapy and by HIV-1 Tat transgenic approach. miR-34a inhibition could effectively inhibit both HIV-Tat protein and antiretroviral therapy-induced vascular aging in mice. The increased miR-34a was induced via p53, whereas Sirt1 was a downstream target gene of miR-34a in both HIV-Tat protein and antiretroviral agents-treated ECs and vessels. The study has demonstrated that miR-34a is a common link in both HIV and antiretroviral therapy-mediated vascular aging.

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“…To quantify blood flow velocity and endothelial function, line scan analyses were performed to trace red blood cell displacement and to calculate the velocity of erythrocytes based on the distance they were displaced per time unit 14,83 . Using the difference in blood flow velocities measured before and during acetylcholine injection, ∆velocity was calculated 85 . Analyzing vessel proximity to CD11b + myeloid cells relied on three to four images of an identified vessel of interest in a z stack.…”

Section: Confocal Microscopymentioning

confidence: 99%

Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease

et al. 2021

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Abnormal hematopoiesis advances cardiovascular disease by generating excess inflammatory leukocytes that attack the arteries and the heart. The bone marrow niche regulates hematopoietic stem cell proliferation and hence the systemic leukocyte pool, but whether cardiovascular disease affects the hematopoietic organ’s microvasculature is unknown. Here we show that hypertension, atherosclerosis and myocardial infarction (MI) instigate endothelial dysfunction, leakage, vascular fibrosis and angiogenesis in the bone marrow, altogether leading to overproduction of inflammatory myeloid cells and systemic leukocytosis. Limiting angiogenesis with endothelial deletion of Vegfr2 (encoding vascular endothelial growth factor (VEGF) receptor 2) curbed emergency hematopoiesis after MI. We noted that bone marrow endothelial cells assumed inflammatory transcriptional phenotypes in all examined stages of cardiovascular disease. Endothelial deletion of Il6 or Vcan (encoding versican), genes shown to be highly expressed in mice with atherosclerosis or MI, reduced hematopoiesis and systemic myeloid cell numbers in these conditions. Our findings establish that cardiovascular disease remodels the vascular bone marrow niche, stimulating hematopoiesis and production of inflammatory leukocytes.

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Guidelines for Assessing Mouse Endothelial Function via Ultrasound Imaging: a Report from the International Society of Cardiovascular Translational Research

Cited by 8 publications

References 13 publications

Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease

Multi-Scale Imaging of Vascular Pathologies in Cardiovascular Disease

miR-34a is a common link in both HIV- and antiretroviral therapy-induced vascular aging

Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease

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