Guggulsterone induces apoptosis and inhibits lysosomal-dependent migration in human bladder cancer cells

Chen, Ying; Wang, Hisao-Hsien; Chang, Hsin‐Han; Huang, Yun-Hsuan; Wang, Jeffrey R.; Changchien, Chih‐Ying; Wu, Sheng‐Tang

doi:10.1016/j.phymed.2021.153587

Cited by 11 publications

(12 citation statements)

References 20 publications

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“…Our flow cytometry and western blotting analyses suggested that Z-GS induced the cell cycle arrest and apoptosis in TNBC cells in a concentration-dependent manner. This observation was consistent with previous studies. , …”

Section: Discussionsupporting

confidence: 94%

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Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer

Zhou

et al. 2023

ACS Omega

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Myrrh is the dried resin of Commiphora Myrrh Engl., which exerts anticancer properties. However, its effects and molecular mechanisms in triple-negative breast cancer (TNBC) remain unclear. In this study, we used network pharmacology to screen Z-Guggulsterone (Z-GS) as a characteristic active component of myrrh. Cell Counting Kit-8 proliferation assays showed that Z-GS inhibited proliferation of the TNBC cell lines MDA-MB-468 and BT-549. Transwell assays also showed that Z-GS inhibited TNBC migration and invasion phenotypes. Our network pharmacology combined with RNA-sequencing analyses showed that Z-GS affected cell cycle and apoptosis processes in TNBC cells, mainly via p53 signaling, to regulate key CCNB1 (cyclin B1), PLK1 (pololike kinase 1), and p53 targets. Flow cytometry revealed that Z-GS arrested the cell cycle at the G2/M phase and increased apoptosis in TNBC cells. Western blotting and quantitative real-time polymerase chain reaction studies confirmed that Z-GS functioned via the p53mediated downregulation of CCNB1 and PLK1 expression. In vivo studies showed that Z-GS effectively inhibited TNBC progression. Collectively, Z-GS exhibited potential anti-TNBC activity and may functions via the p53/CCNB1/PLK1 pathway.

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Section: Discussionsupporting

confidence: 94%

“…This observation was consistent with previous studies. 24,25 P53 is an essential transcription factor required for genomic integrity maintenance. The p53 pathway has vital roles in preventing cancer formation and is ubiquitously lost in a high percentage of human cancers.…”

Section: Discussionmentioning

confidence: 99%

Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer

Zhou

et al. 2023

ACS Omega

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“…Therefore, the induction of apoptosis in tumor cells may be a new approach for tumor treatment. Chen et al revealed that guggulsterone inhibits BLCA growth and metastasis through caspase-dependent apoptosis [ 37 ]. Yin et al showed that epigallocatechin-3-gallate can inhibited bladder cancer cells via inducing autophagy-related apoptosis [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Apoptosis related genes mediated molecular subtypes depict the hallmarks of the tumor microenvironment and guide immunotherapy in bladder cancer

Zhou

et al. 2023

BMC Med Genomics

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Apoptosis has been discovered as a mechanism of cell death. The purpose of this study is to identify the diagnostic signature factors related to bladder cancer (BLCA) through apoptosis related genes (ARGs). Clinicopathological parameters and transcriptomics data of 1,440 BLCA patients were obtained from 7 datasets (GSE13507, GSE31684, GSE32548, GSE32894, GSE48075, TCGA-BLCA, and IMvigor210). We first identified prognosis-related ARGs in BLCA and used them to construct two ARGs molecular subtypes by using consensus clustering algorithm. By using principal component analysis algorithms, a ARGscore was constructed to quantify the index of individualized patient. High ARGscore correlated with progressive malignancy and poor outcomes in BLCA patients. High ARGscore was associated with higher immune cell, higher estimate scores, higher stromal scores, higher immune scores, higher immune checkpoint, and lower tumor purity, which was consistent with the “immunity tidal model theory”. Preclinically, BLCA immunotherapy cohorts confirmed patients with low ARGscore demonstrated significant therapeutic advantages and clinical benefits. These findings contribute to our understanding of ARGs and immunotherapy in BLCA. The ARGscore is a potentially useful tool to predict the prognosis and immunotherapy in BLCA.

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“…By binding to LC3-II, p62 is incorporated into autophagosomes and then destroyed during autophagy. As a result, p62 is negatively correlated with autophagy ( 467 , 468 ). New studies have shown the involvement of autophagy in cardiac function, and have suggested that induction of autophagy could enhance heart function during sepsis ( 469 , 470 ).…”

Section: Melatonin As a Protective Agent Against Septic Cardiomyopathymentioning

confidence: 99%

Evidence for the Benefits of Melatonin in Cardiovascular Disease

Tobeiha

Jafari

Fadaei

et al. 2022

Front. Cardiovasc. Med.

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The pineal gland is a neuroendocrine gland which produces melatonin, a neuroendocrine hormone with critical physiological roles in the circadian rhythm and sleep-wake cycle. Melatonin has been shown to possess anti-oxidant activity and neuroprotective properties. Numerous studies have shown that melatonin has significant functions in cardiovascular disease, and may have anti-aging properties. The ability of melatonin to decrease primary hypertension needs to be more extensively evaluated. Melatonin has shown significant benefits in reducing cardiac pathology, and preventing the death of cardiac muscle in response to ischemia-reperfusion in rodent species. Moreover, melatonin may also prevent the hypertrophy of the heart muscle under some circumstances, which in turn would lessen the development of heart failure. Several currently used conventional drugs show cardiotoxicity as an adverse effect. Recent rodent studies have shown that melatonin acts as an anti-oxidant and is effective in suppressing heart damage mediated by pharmacologic drugs. Therefore, melatonin has been shown to have cardioprotective activity in multiple animal and human studies. Herein, we summarize the most established benefits of melatonin in the cardiovascular system with a focus on the molecular mechanisms of action.

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Guggulsterone induces apoptosis and inhibits lysosomal-dependent migration in human bladder cancer cells

Cited by 11 publications

References 20 publications

Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer

Z-Guggulsterone Induces Cell Cycle Arrest and Apoptosis by Targeting the p53/CCNB1/PLK1 Pathway in Triple-Negative Breast Cancer

Apoptosis related genes mediated molecular subtypes depict the hallmarks of the tumor microenvironment and guide immunotherapy in bladder cancer

Evidence for the Benefits of Melatonin in Cardiovascular Disease

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