(GTG)5-PCR fingerprinting of multi-drug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso

Abstract

Background Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform molecular typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiological tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR. Results Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). Conclusion The (GTG) 5-PCR assay enabled rapid molecular typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones.

High prevalence of multidrug resistant and extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from urinary tract infections in West region, Cameroon.

“…High prevalence of uropathogens have been reported in sub-Saharan Africa (sSA) with 89.17% in Nigeria, 39.13% in Uganda, 10.1% in Ghana and 21.2% among children in Gambia [3]. These reports shown that most species belonged to Enterobacterales including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and were associated with numerous risk factors such as age, poor economic status, poor hygiene, hospitalization, catheterization, sexual activities, pregnancy and diabetes mellitus [3][4][5].…”

“…High prevalence of uropathogens have been reported in sub-Saharan Africa (sSA) with 89.17% in Nigeria, 39.13% in Uganda, 10.1% in Ghana and 21.2% among children in Gambia [3]. These reports shown that most species belonged to Enterobacterales including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and were associated with numerous risk factors such as age, poor economic status, poor hygiene, hospitalization, catheterization, sexual activities, pregnancy and diabetes mellitus [3][4][5].Emergence and escalation of extended-spectrum β-lactamase-producing E. coli and K. pneumoniae is aggravating the global concern of urinary tract infections. They are commonly associated with increased length of hospital stay, use of last-resort and often expensive drug and increase mortality [6].…”

Clinical and epidemiological characteristics of multi-drug resistant Enterobacterales isolated from King Fahad Hospital of the University, AlKhobar, Saudi Arabia

“…All strains collected in this study were MDR (non-susceptible to at least one agent in three or more antibiotic classes) [ 11 ]. Control strains, E. coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, and Pseudomonas aeruginosa ATCC 27853, were included in every antibiotic susceptibility testing (AST) run [ 12 ]. All collected strains exhibited an MDR profile defined by the isolate being non-susceptible to at least one agent in ≥ three antimicrobial categories.…”

Entomotherapy as an alternative treatment for diseases due to Gram-negative bacteria in Burkina Faso

“…This means that Gram-positive and Gram-negative bacteria require different treatments. These carbapenemase and beta-lactamase-producing bacteria are common in West Africa 17 . However, the spread of Gram-negative bacteria producing carbapenemases and extended-spectrum beta-lactamases constitutes an “urgent” threat.…”