(GTG)5-PCR fingerprinting of lactobacilli isolated from cervix of healthy women

Abstract

A group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (GTG)(5)-polymerase chain reaction (PCR) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. Obtained fingerprints were compared with profiles available in an in-house database of the CCM bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. Selected strains representing individual clusters were further identified by pheS gene sequencing. In total, six lactobacillus species were found among lactobacilli isolated from the cervix of healthy women. The (GTG)(5)-PCR method identified Lactobacillus gasseri (11 strains), Lactobacillus fermentum (one), and some of the Lactobacillus jensenii strains (eight out of 11), but failed to identify the remaining strains, including the Lactobacillus crispatus (18), Lactobacillus mucosae (one), and Lactobacillus vaginalis (one) species. L. jensenii strains were distributed over two fingerprint clusters. The majority of samples was dominated by one (GTG)(5)-PCR type. The rep-PCR fingerprinting using the (GTG)(5) primer allowed straightforward identification of many, but not all, isolates. This method has been shown to be a useful tool for fast screening and grouping of vaginal lactobacilli, but its combination with another identification method is needed to obtain reliable identification results. In addition, Lactobacillus acidophilus was not shown to be the most common inhabitant of the female genital tract as generally assumed.

Identification, Classification and Screening for γ-Amino-butyric Acid Production in Lactic Acid Bacteria from Cambodian Fermented Foods

“…Both species are closely related [26]. As previously reported, these results demonstrate that (GTG) 5 -PCR fingerprinting is a useful tool for grouping lactobacilli [20,47,48].…”

Comparative Genomics Reveals Biomarkers to Identify Lactobacillus Species

“…This study revealed that although (GTG)(5)-PCR helps in identifying many isolates, however, a supplementary gene information is needed for reliable identification. An interesting finding of this study was the fact that Lactobacillus acidophilus is not a common inhabitant of female genital tract [35]. After an initial identification as Lactobacillus sp.…”

(GTG)5-PCR Mediated Molecular Typing of Zoonotic Bacteria

“…Nováková et al (2010) By using rep-PCR using the (GTG)5 primer could differentiate Staphylococcus microti DSM 22147 from viscera of common voles. Svec et al (2011) used the (GTG) 5 -PCR to characterize vaginal lactobacilli. It is declared that (GTG) 5 -PCR is a simple, beneficial, and rapid method for the identification of many vaginal lactobacilli isolates and also suggested that this method can be more reliable in combination with other techniques (Svec et al, 2008;Svec et al, 2011).…”