Growth, Survival, and Feeding Rates for the Geoduck<i>Panopea globosa</i>During Larval Development

Ferreira-Arrieta, Alejandra; García-Esquivel, Zaúl; González-Gómez, Marco A.; Valenzuela-Espinoza, Enrique

doi:10.2983/035.034.0108

Cited by 13 publications

(17 citation statements)

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“…Proteins in cluster 10 (enriched for glucan metabolism) begin a steady increase in abundance starting at day 10, further indicating a relationship with metamorphosis and energetic demands. Other studies have demonstrated ingestion rate increases throughout larval development in the geoduck Panopea globosa (Ferreira-Arrieta, García-Esquivel, González-Gómez, & Valenzuela-Espinoza, 2015), which corresponds to the proteomic signatures observed. Changes in abundances of digestion enzymes were also observed in B. areolata as feeding strategies shifted throughout development (Shen et al, 2018).…”

Section: Discussionsupporting

confidence: 65%

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂

Timmins‐Schiffman

Guzmán

Thompson

et al. 2019

Ecology and Evolution

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Pacific geoducks (Panopea generosa) are clams found along the northeast Pacific coast where they are important components of coastal and estuarine ecosystems and a major aquaculture product. The Pacific coastline, however, is also experiencing rapidly changing ocean habitat, including significant reductions in pH. To better understand the physiological impact of ocean acidification on geoduck clams, we characterized for the first time the proteomic profile of this bivalve during larval development and compared it to that of larvae exposed to low pH conditions. Geoduck larvae were reared at pH 7.5 (ambient) or pH 7.1 in a commercial shellfish hatchery from day 6 to day 19 postfertilization and sampled at six time points for an in‐depth proteomics analysis using high‐resolution data‐dependent analysis. Larvae reared at low pH were smaller than those reared at ambient pH, especially in the prodissoconch II phase of development, and displayed a delay in their competency for settlement. Proteomic profiles revealed that metabolic, cell cycle, and protein turnover pathways differed between the two pH and suggested that differing phenotypic outcomes between pH 7.5 and 7.1 are likely due to environmental disruptions to the timing of physiological events. In summary, ocean acidification results in elevated energetic demand on geoduck larvae, resulting in delayed development and disruptions to normal molecular developmental pathways, such as carbohydrate metabolism, cell growth, and protein synthesis.

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Section: Discussionsupporting

confidence: 65%

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂

Timmins‐Schiffman

Guzmán

Thompson

et al. 2019

Ecology and Evolution

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“…The flagellate Isochrysis clone T‐ISO has been successfully used as single diet for all stages of Panopea sp . (Garcia‐Esquivel et al., , Ferreira‐Arrieta et al., ; Tapia‐Morales, García‐Esquivel, Vadopalas, & Davis, ) and proved to be a good food source for P. generosa larvae (Liu, Pearce, McKinley & Forster, ). Water and food supply rates were the same for all nine tanks.…”

Section: Methodsmentioning

confidence: 99%

“…Allometric relationships between shell length (SL) and ash‐free dry weight (AFDW) of P. generosa larvae were established prior to the estimation of IR using a different larval batch, and following the protocols described by Ferreira‐Arrieta et al. (). Both variables were log–transformed in order to obtain a regression line and the appropriate constants.…”

Section: Methodsmentioning

confidence: 99%

“…The experimentation and development of this technology started in Mexico in 2008 (Garcia‐Esquivel, Buitimea‐Alvarez, Valenzuela‐Espinoza, Garduño & Ley‐Lou, ). Today, standard techniques are available for the production of P. globosa and P. generosa larvae in cylindrical cone bottom tanks using the traditional batch culture method (Ferreira‐Arrieta, García‐Esquivel, González‐Gómez & Valenzuela‐Espinoza, ), as well as the settlement on nylon mesh and further post‐larval rearing in sediment‐free “raceway” systems (Garcia‐Esquivel et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…Production of Panopea generosa larvae in the Mollusc Biotechnology Laboratory at the Universidad Autonoma de Baja California is carried out in 500 L tanks using the batch culture system (Garcia‐Esquivel et al., ), with full water renewal every other day and larval stocking densities similar to those of P. globosa (Ferreira‐Arrieta et al., ). For this reason, the present study was carried out in order to: (1) investigate the effectiveness of a continuous flow‐through system using a head level tank to regulate water flow, and (2) evaluate the performance of Panopea generosa larvae (survival and growth) reared in these systems at different stocking densities.…”

Section: Introductionmentioning

confidence: 99%

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Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

et al. 2017

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The geoduck clam, Panopea generosa, is a species from the west coast of Baja California, Mexico, and the optimization of seed production systems is still a limiting factor for its aquaculture. In this study, a flow-through culture system was designed and tested in P. generosa larvae. Survival and growth was compared in triplicate 45-L fiberglass tanks using three larval densities (5, 10 and 15 larvae/ml). A head tank kept constant the water inflow, with a daily renewal rate of 1.89 tank volume. The food (Isochrysis spp.) was dosed according to the ingestion rate of larvae and the dilution rate. Survival decreased linearly during the first 10 days and reached asymptotic values of ca. 20% (15 larvae/ml) and 50% (densities of 5 and 10 larvae/ ml) afterwards. Mean shell length at the end of the experiment (243 AE 1.8 to 270 AE 0.7 lm) was not statistically different among treatments, even though a trend towards higher gross growth rate was observed in the treatment with the lowest density (9.5 lm/day) relative to the rest of the treatments (8.5 lm/day). It is concluded that P. generosa larvae can be successfully grown in flow-through systems at maximum densities of 10 larvae/ml without significantly affecting their survival and growth rates. The system design was reliable, kept a constant water flow with reduced maintenance, and may represent an important option in the laboratory for increasing the stocking density of Panopea species during the larval phase. K E Y W O R D Sbivalve larvae, flow-through, geoduck culture, larval culture, Panopea

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Functional morphology and performance of New Zealand geoduck clam ( Panopea zelandica ) larvae reared in a flow-through system

Ragg

Hilton

et al. 2017

Aquaculture

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Growth, Survival, and Feeding Rates for the GeoduckPanopea globosaDuring Larval Development

Cited by 13 publications

References 20 publications

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂

Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

Functional morphology and performance of New Zealand geoduck clam ( Panopea zelandica ) larvae reared in a flow-through system

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Growth, Survival, and Feeding Rates for the GeoduckPanopea globosaDuring Larval Development

Cited by 13 publications

References 20 publications

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO2

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO2

Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

Functional morphology and performance of New Zealand geoduck clam ( Panopea zelandica ) larvae reared in a flow-through system

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Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂

Dynamic response in the larval geoduck (Panopea generosa) proteome to elevated pCO₂