Functional morphology and performance of New Zealand geoduck clam ( Panopea zelandica ) larvae reared in a flow-through system

Le, Dung V.; Ragg, Norman L.C.; Hilton, Zoë; Watts, Ellie; King, Nathan

doi:10.1016/j.aquaculture.2016.09.047

Cited by 13 publications

(11 citation statements)

References 32 publications

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“…The recent study reported by Le et al . () for P. zelandica stocked at high densities with water renewal rates of 46 volumes/day supports this hypothesis (Table ).…”

Section: Discussionsupporting

confidence: 67%

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Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

et al. 2017

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The geoduck clam, Panopea generosa, is a species from the west coast of Baja California, Mexico, and the optimization of seed production systems is still a limiting factor for its aquaculture. In this study, a flow-through culture system was designed and tested in P. generosa larvae. Survival and growth was compared in triplicate 45-L fiberglass tanks using three larval densities (5, 10 and 15 larvae/ml). A head tank kept constant the water inflow, with a daily renewal rate of 1.89 tank volume. The food (Isochrysis spp.) was dosed according to the ingestion rate of larvae and the dilution rate. Survival decreased linearly during the first 10 days and reached asymptotic values of ca. 20% (15 larvae/ml) and 50% (densities of 5 and 10 larvae/ ml) afterwards. Mean shell length at the end of the experiment (243 AE 1.8 to 270 AE 0.7 lm) was not statistically different among treatments, even though a trend towards higher gross growth rate was observed in the treatment with the lowest density (9.5 lm/day) relative to the rest of the treatments (8.5 lm/day). It is concluded that P. generosa larvae can be successfully grown in flow-through systems at maximum densities of 10 larvae/ml without significantly affecting their survival and growth rates. The system design was reliable, kept a constant water flow with reduced maintenance, and may represent an important option in the laboratory for increasing the stocking density of Panopea species during the larval phase. K E Y W O R D Sbivalve larvae, flow-through, geoduck culture, larval culture, Panopea

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“…The recent study reported by Le et al . () for P. zelandica stocked at high densities with water renewal rates of 46 volumes/day supports this hypothesis (Table ).…”

Section: Discussionsupporting

confidence: 67%

“…It was also recently reported a high survival per cent (>70%) of Panopea zelandica larvae reared in 2.5 L containers at stocking densities between 50 and 200 organisms/ml, with water renewal rates of ca. 50 volumes/day (Le et al., ).…”

Section: Discussionmentioning

confidence: 99%

Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

et al. 2017

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“…Regarding fertilization, in our experience, the ratio of ≥ 100:1 sperm:oocytes showed over 75% success in fecundation, and, in the 1000:1 sperm:oocytes treatment, oocytes in lysis were registered. Similarly, Le et al [ 21 ] established an optimal ratio < 500:1 sperm:oocytes for Panopea zelandica , finding no significant differences in the percentage of embryos at 18 h pf between the ratio of 50:1 and 500:1; however, the ratio 50:1 showed a higher percentage (96%) of normal embryos. In our study, the result was the opposite, with greater fertilization obtained at higher sperm:oocyte ratios.…”

Section: Discussionmentioning

confidence: 96%

“…The success of fertilization depends primarily on a good gametic ratio of sperm:oocytes, and this ratio depends on the endogenous and exogenous factors that regulate different processes in the reproductive cycle. Low sperm concentrations reduce the likelihood of oocyte encounters, and high sperm concentrations can increase the risk of polyspermy, which can lead to abnormal embryo development, affecting larval survival [ 19 , 20 , 21 , 23 ]. This optimal ratio varies between species of bivalves.…”

Section: Discussionmentioning

confidence: 99%

“…The success of fertilization depends on the sperm:oocyte ratio, where low concentrations of sperm reduce the probability of encounters with the oocyte, and a high concentration of sperm can increase the risk of polyspermy that generates an abnormal development of the embryos and affects the survival of larvae [ 19 , 20 , 21 , 22 , 23 ]. On the other hand, physical factors, such as temperature, shape the speed of development, growth rate and mortality of embryos and larvae and thus are some of the most relevant factors for the success of marine cultures [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Sperm Ratio and Temperature on Fertilization and Early Larval Development of the Surf Clam Mesodesma donacium (Bivalvia:Mesodesmatidae)

Vásquez-Calderón

Abarca

Durán

et al. 2022

Animals

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The effect of sperm ratio on fertilization was evaluated in five sperm:oocytes treatments (10:1, 50:1, 100:1, 500:1 and 1000:1), the effect of temperature on embryonic and larval development in three temperature treatments (13 °C, 16 °C and 19 °C) was recorded and the duration of each stage, the growth rate and survival rate were registered. The oocytes were spherical (67.5 ± 4.2 μm) with a defined nucleus. Spermatozoa had a circular head (2 μm) and a fusiform flagellum (12 μm). The 500:1 sperm:oocytes treatment presented the lowest % of unfertilized oocytes, and lysis was observed in the 1000:1 treatment. An inverse relationship was observed between temperature and the duration of the stages of embryonic development. At 16 °C, veliger D larvae were observed at 41 h 45′ pf (88 ± 13.0 μm). Umbonate larvae were obtained at day 16 in the 13 °C culture and at day 10 in the 16 °C and 19 °C treatment (140 μm). On day 16 of culture, advanced umbonate larvae with a well-defined stomach (235 μm) were observed. The larval growth rate was higher in the 19 °C treatment (3.6 μm day−1) than the 13 °C and 16 °C treatment (2, 2.2 μm day−1). The mortality was higher in the 19 °C treatment (91%). These results are an initial contribution towards the culture of M. donacium as part of small-scale aquaculture in South America.

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Hatchery practices for Patella aspera and P. ordinaria: evaluating culture vessels requirements

Castejón,

Sousa,

Andrade

2024

Aquacult Int

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Innovation in limpet production requires knowledge of the optimal qualities and limitations of the culture vessels. Two limpet species from Madeira fishery, Patella aspera and P. ordinaria, were used to evaluate the influence of culture vessel shape (microtubes, test tubes, centrifuge tubes, specimen cups, disposable cups, vials, flasks and beakers), composition (polypropylene and glass), volume (1.5 ml to 1.5 L) and stocking density on oocyte incubation and larval culture through nine assays. Moreover, three assays evaluated the use of microtubes and the isolation of trochophores, instead of pediveligers, for eventual settlement induction. The results showed effective hatching in most culture vessels regardless of composition or volume, except when the shape of the bottom caused oocyte clustering. Larval culture was not influenced by any variable under scrutiny (shape, composition, volume or rearing density). Microtubes were useful for settlement, while the placement of trochophores in the settling vessels, instead of pediveligers, proved inefficient and even led to failed cultures. The best settlement was obtained when the pediveligers were isolated from the water column of the beakers and transferred to clean vessels with renewed water and coralline algae as substrate. The current findings could be extrapolated to other marine invertebrates that share similar life stage requirements.

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Functional morphology and performance of New Zealand geoduck clam ( Panopea zelandica ) larvae reared in a flow-through system

Cited by 13 publications

References 32 publications

Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

Survival and growth of geoduck clam larvae (Panopea generosa ) in flow-through culture tanks under laboratory conditions

Effect of Sperm Ratio and Temperature on Fertilization and Early Larval Development of the Surf Clam Mesodesma donacium (Bivalvia:Mesodesmatidae)

Hatchery practices for Patella aspera and P. ordinaria: evaluating culture vessels requirements

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