Growth responses of blue-green algae to sodium chloride concentration

BattertonJr., John C.; Baalen, Chase Van

doi:10.1007/bf00411789

Cited by 110 publications

(46 citation statements)

References 41 publications

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“…It uses nitrate, ammonium or urea as a nitrogen source, and is auxotrophic for vitamin B 12 , which is required for methionine biosynthesis and photosynthetic activity at low temperatures (Batterton and Vanbaalen, 1971;Rippka et al, 1979). Conversely, Shewanella W3-18-1 (Nealson and Scott, 2003) is a facultative anaerobe that possesses a versatile heterotrophic metabolism and can utilize a wide array of carbon sources including various carboxylic acids, carbohydrates, amino acids and nucleic acids, a range of di-peptides as nitrogen sources, and DNA as a phosphorus source Rodionov et al, 2010;Rodrigues et al, 2011).…”

Section: Resultsmentioning

confidence: 99%

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing

et al. 2014

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We used deep sequencing technology to identify transcriptional adaptation of the euryhaline unicellular cyanobacterium Synechococcus sp. PCC 7002 and the marine facultative aerobe Shewanella putrefaciens W3-18-1 to growth in a co-culture and infer the effect of carbon flux distributions on photoautotroph-heterotroph interactions. The overall transcriptome response of both organisms to co-cultivation was shaped by their respective physiologies and growth constraints. Carbon limitation resulted in the expansion of metabolic capacities, which was manifested through the transcriptional upregulation of transport and catabolic pathways. Although growth coupling occurred via lactate oxidation or secretion of photosynthetically fixed carbon, there was evidence of specific metabolic interactions between the two organisms. These hypothesized interactions were inferred from the excretion of specific amino acids (for example, alanine and methionine) by the cyanobacterium, which correlated with the downregulation of the corresponding biosynthetic machinery in Shewanella W3-18-1. In addition, the broad and consistent decrease of mRNA levels for many Fe-regulated Synechococcus 7002 genes during co-cultivation may indicate increased Fe availability as well as more facile and energy-efficient mechanisms for Fe acquisition by the cyanobacterium. Furthermore, evidence pointed at potentially novel interactions between oxygenic photoautotrophs and heterotrophs related to the oxidative stress response as transcriptional patterns suggested that Synechococcus 7002 rather than Shewanella W3-18-1 provided scavenging functions for reactive oxygen species under co-culture conditions. This study provides an initial insight into the complexity of photoautotrophic-heterotrophic interactions and brings new perspectives of their role in the robustness and stability of the association.

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Section: Resultsmentioning

confidence: 99%

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing

et al. 2014

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“…strain PCC 7002 (hereafter Synechococcus 7002). This organism has become a widely studied model system for investigating cyanobacterial photophysiology and metabolism, because it is capable of growth over a wide range of NaCl concentrations (0–2.0 M) and under high irradiance levels (Batterton and Van Baalen, 1971). Its tolerance to very high irradiance (Nomura et al, 2006) and resistance to reactive oxygen species have been correlated, at least in part, to very high constitutive transcript levels encoding putative protective enzymes (Ludwig and Bryant, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002

et al. 2014

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Synechococcus sp. PCC 7002 was grown to steady state in optically thin turbidostat cultures under conditions for which light quantity and quality was systematically varied by modulating the output of narrow-band LEDs. Cells were provided photons absorbed primarily by chlorophyll (680 nm) or phycocyanin (630 nm) as the organism was subjected to four distinct mono- and dichromatic regimes. During cultivation with dichromatic light, growth rates were generally proportional to the total incident irradiance at values <275 μmol photons m−2 · s−1 and were not affected by the ratio of 630:680 nm wavelengths. Notably, under monochromatic light conditions, cultures exhibited similar growth rates only when they were irradiated with 630 nm light; cultures irradiated with only 680 nm light grew at rates that were 60–70% of those under other light quality regimes at equivalent irradiances. The functionality of photosystem II and associated processes such as maximum rate of photosynthetic electron transport, rate of cyclic electron flow, and rate of dark respiration generally increased as a function of growth rate. Nonetheless, some of the photophysiological parameters measured here displayed distinct patterns with respect to growth rate of cultures adapted to a single wavelength including phycobiliprotein content, which increased under severely light-limited growth conditions. Additionally, the ratio of photosystem II to photosystem I increased ~40% over the range of growth rates, although cells grown with 680 nm light only had the highest ratios. These results suggest the presence of effective mechanisms which allow acclimation of Synechococcus sp. PCC 7002 acclimation to different irradiance conditions.

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“…Accumulation ratios of interna1 to external inorganic carbon concentrations of 1000 or more have been found (Badger and Gallagher, 1987;Kaplan et al, 1980;Salon et al, 1996a, 199613). Na+ is an essential element for cyanobacteria (Kratz and Meyers, 1955;Fogg, 1956;Batterton and Van Baalen, 1971). Synechococcus will not grow in culture in alkaline conditions (pH, 9.0 or higher) unless Nat is present (Miller et al, 1984).…”

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Driving Forces for Bicarbonate Transport in the Cyanobacterium Synechococcus R-2 (PCC 7942)

1996

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Air-grown Synechococcus R-2 (PCC 7942) cultures grown in BC-11 medium are very alkaline (outside pH is 10.0) and use HC0,-as their inorganic carbon source. The cells showed a dependente on Na+ for photosynthesis, but low Na+ conditions (1 mo1 m-3) were sufficient to support saturating photosynthesis. The intracellular dissolved inorganic carbon in the light was greater than 20 mo1 m-3 in both low-Na+ conditions and in BC-11 medium containing the usual [Na+] (24 mo1 m-,, designated high-Na+ conditions). l h e electrochemical potential for HC0,-in the light was in excess of 25 kJ mol-', even in high-Na+ conditions. The Na+-motive force was greater than -12 kJ mol-' under both Na+ conditions. On thermodynamic grounds, an Na+-driven co-port process would need to have a stoichiometry of 2 or greater (22Na+i,,/HC03-'i,), but we show that Nat or K+ fluxes cannot be linked to HC0,-transport. Na+ and K+ fluxes were unaffected by the presence or absence of dissolved inorganic carbon. In low-Na+ conditions, Na+ fluxes are too low to support the observed net C-carbon fixation rate. Active transport of HC0,-hyperpolarizes (not depolarizes) the membrane potential. 14

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Growth responses of blue-green algae to sodium chloride concentration

Cited by 110 publications

References 41 publications

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing

Inference of interactions in cyanobacterial–heterotrophic co-cultures via transcriptome sequencing

Effect of mono- and dichromatic light quality on growth rates and photosynthetic performance of Synechococcus sp. PCC 7002

Driving Forces for Bicarbonate Transport in the Cyanobacterium Synechococcus R-2 (PCC 7942)

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