Growth-regulated formation of heteromeric complexes of the centromere and promoter factor, Cbf1p, in yeast

Oechsner, Ulrich; Bandlow, Wolfhard

doi:10.1007/s004380050912

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…Thus, it seems very likely that the protein is able to form homodimers or heterodimers with as yet unknown partner proteins or can be part of a complex, as shown for other bHLH proteins, e.g. Myc or Cbf1 (Oechsner and Bandlow, 1998; Hemmerich et al ., 2000; Fuchs et al ., 2001). Potential partners could be other bHLH proteins such as StuA or AnBH1.…”

Section: Discussionmentioning

confidence: 95%

A basic‐region helix–loop–helix protein‐encoding gene (devR) involved in the development of Aspergillus nidulans

Tüncher

Reinke

Martic

et al. 2004

Molecular Microbiology

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SummaryBasic-region helix-loop-helix (bHLH) proteins form an interesting class of eukaryotic transcription factors often involved in developmental processes. Here, a so far unknown bHLH protein-encoding gene of the filamentous ascomycete Aspergillus nidulans was isolated and designated devR for regulator of development. Deletion of devR revealed that the gene is non-essential for vegetative growth. However, the deletion mutant produced wrinkled colonies, a yellow pigment and did not form conidia on minimal agar plates. Conidiophore development was initiated normally, and colonies produced conidiophores with metulae and phialides. However, the phialides continued to grow filamentously and produced a second conidiophore with a vesicle at its end. The addition of KCl (0.6 M) to the medium suppressed the knock-out phenotype. The D D D D devR phenotype resembled that of a mutation in the tcsA gene encoding a histidine kinase domain and a response regulator domain. Here, we generated a tcsA deletion mutant. In a D D D D tcsA strain, a DevR-Egfp protein fusion was detected in the cytoplasm, whereas in the wild type, the protein fusion was exclusively located in the nuclei, indicating that TcsA is required for nuclear localization of DevR. devR mRNA steady-state levels were similar in sporulating and vegetatively growing mycelia, and independent of a functional brlA gene. Moreover, under all conditions tested, self-crossing of the D D D D devR mutant strain was never observed. Taken together, devR encodes a bHLH regulatory protein that is part of the tcsA signal transduction network and required for development under standard growth conditions.

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Section: Discussionmentioning

confidence: 95%

A basic‐region helix–loop–helix protein‐encoding gene (devR) involved in the development of Aspergillus nidulans

Tüncher

Reinke

Martic

et al. 2004

Molecular Microbiology

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“…Met4p is not the only example of a factor known to interact with Cbf1p to form stable DNA-bound complexes at promoters. For instance, a number of growth condition-dependent complexes at the CYT1 promoter have been reported (56).…”

Section: Figmentioning

confidence: 99%

Cbf1p Is Required for Chromatin Remodeling at Promoter-proximal CACGTG Motifs in Yeast

Kent

Eibert

Mellor

2004

Journal of Biological Chemistry

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Cbf1p is a basic-helix-loop-helix-zipper protein of Saccharomyces cerevisiae required for the function of centromeres and MET gene promoters, where it binds DNA via the consensus core motif CACRTG (R ‫؍‬ A or G). At MET genes Cbf1p appears to function in both activator recruitment and chromatin-remodeling. Cbf1p has been implicated in the regulation of other genes, and CACRTG motifs are common in potential gene regulatory DNA. A recent genome-wide location analysis showed that the majority of intergenic CACGTG palindromes are bound by Cbf1p. Here we tested whether all potential Cbf1p binding motifs in the yeast genome are likely to be bound by Cbf1p using chromatin immunoprecipitation. We also tested which of the motifs are actually functional by assaying for Cbf1p-dependent chromatin remodeling. We show that Cbf1p binding and activity is restricted to palindromic CACGTG motifs in promoter-proximal regions. Cbf1p does not function through CACGTG motifs that occur in promoter-distal locations within coding regions nor where CACATG motifs occur alone except at centromeres. Cbf1p can be made to function at promoter-distal CACGTG motifs by overexpression, suggesting that the concentration of Cbf1p is normally limiting for binding and is biased to gene regulatory DNA by interactions with other factors. We conclude that Cbf1p is required for normal nucleosome positioning wherever the CACGTG motif occurs in gene regulatory DNA. Cbf1p has been shown to interact with the chromatin-remodeling ATPase Isw1p. Here we show that recruitment of Isw1p by Cbf1p is likely to be general but that Isw1p is only partially required for Cbf1p-dependent chromatin structures.

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“…This factor is encoded by the nonessential gene CBF1 (Baker and Masison 1990; Mellor et al 1990). The role of Cbf1 is not limited to the centromere, but also includes the transcriptional activation of certain genes transcribed by RNAPII (Baker and Masison 1990;Mellor et al 1990;Blaiseau and Thomas 1998;Oechsner and Bandlow 1998). Both Cep3 and Skp1 are members of the Cbf3 complex that binds to the CDEIII region of the centromere (reviewed in Clarke 1998).…”

Section: Genetic Interactions With Cen Binding Protein Mutantsmentioning

confidence: 99%

Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae

Pierstorff

Kane

2004

Mol Genet Genomics

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The Saccharomyces cerevisiase protein phosphatase Fcp1 has been implicated in the regulation of transcription by RNA polymerase II, and is encoded by the essential gene FCP1. A screen was carried out for multicopy suppressors of the temperature-sensitive phenotype of two phosphatase mutants, fcp1-2 and fcp1-4. Only the wild-type FCP1 was found to suppress (complement) the fcp1-4 mutation. For fcp1-2 three second-site suppressors were identified. One contained the ORF for ZDS1. The remaining two suppressors mapped to the centromere regions of chromosomes I and V. Suppression due to centromere DNA was found to be more dependent on the CDEIII region than on other regions of the centromere. The presence of a suppressor centromere affected the level of Fcp1 protein and the overall phosphorylation state of RNA polymerase II (RNAPII) in fcp1-2 cells, but not wild-type cells, grown at both permissive and non-permissive temperatures. In addition, genetic interactions were identified between this FCP1 mutant and the genes SKP1, CEP3 and CBF1, which code for centromere binding proteins. The mechanism of suppression and regulation of Fcp1-2 protein activity by centromeric DNA is discussed.

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Growth-regulated formation of heteromeric complexes of the centromere and promoter factor, Cbf1p, in yeast

Cited by 6 publications

References 30 publications

A basic‐region helix–loop–helix protein‐encoding gene (devR) involved in the development of Aspergillus nidulans

A basic‐region helix–loop–helix protein‐encoding gene (devR) involved in the development of Aspergillus nidulans

Cbf1p Is Required for Chromatin Remodeling at Promoter-proximal CACGTG Motifs in Yeast

Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae

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