The growth yield of orange-colored Streptococcus boils No. 148 isolated from bovine rumen was investigated. The high growth yield of S. bovis occurred in the presence of C02 under batch culture conditions, and the maximum value for YATP (the gram dry weight of organism produced per mol of ATP) was about 18. The growth yield was decreased by the addition of NaHC03 or Na2C03 which are commonly used as neutralizing agents for the culture of ordinary rumen bacteria. The growth-promoting effect of C02 could be partially replaced by some unsaturated fatty acids and oleate-containing compounds.
The growth yield of orange-colored Streptococcus boils No. 148 isolated from bovine rumen was investigated. The high growth yield of S. bovis occurred in the presence of C02 under batch culture conditions, and the maximum value for YATP (the gram dry weight of organism produced per mol of ATP) was about 18. The growth yield was decreased by the addition of NaHC03 or Na2C03 which are commonly used as neutralizing agents for the culture of ordinary rumen bacteria. The growth-promoting effect of C02 could be partially replaced by some unsaturated fatty acids and oleate-containing compounds.
“…By integration of the area under the curve the total heat Q is determined. Experiments with increasing amounts of trehalose rendered an enthalpy change of AH = -139.7 kJ pro tool glucose [10] in excellent agreement with data from the literature [ 11 ]. This simultaneous determination of heat production rate and total heat enables one to transform the p-t-curve (Fig.…”
Microcalorimetric experiments on the periodic heat production in metabolizing cell free cytoplasmic extract from baker's yeast are described. The sugar substrates for glycolysis are supplied by two different techniques: (a) one-time addition of trehalose; (b) continuous infusion of glucose. For the first time the combination of calorimetry and substrate infusion technique is reported for oscillating biochemical reactions.
“…Earlier attempts to associate the second law with the dynamic components of growth revealed that, if significant calcu lations are to be made, the phenomenon must have explicitly defined initial and final states (6,7,61,147). Battley suggested that the initial state should comprise the enthalpy represented by the components of the growth medium and at least one cell (5) and, further, that the final state should be charac terized by exhaustion of the substrate. To test this hypothesis, a strict ac counting was obtained from cultures of S. cerevisiae for carbon expended from the medium and recovered in the cells and the end products.…”
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