Growth Promotion and Biocontrol Activity of Endophytic Streptomyces spp.

Vurukonda, Sai Shiva Krishna Prasad; Giovanadri, Davide; Stefani, Emilio

doi:10.37247/pamol2ed.2.2021.20

Cited by 5 publications

(7 citation statements)

References 96 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Actinobacteria ( Streptomyces spp. mainly) have been reported as potential BCAs against soil-borne pathogens of legumes during the last decade [ 16 ]. However, the use of actinobacteria as BCAs in the frame of the integrated management of the major diseases of common bean caused by soil-borne pathogens in Cuba has not been explored yet.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to these well-known BCAs, research in the last decades highlights the benefits of the actinobacteria ( Streptomyces spp. mainly) and their potential as BCAs (e.g., Streptomyces griseoviridis , S. lydicus ) against soil-borne pathogens, such as species of Rhizoctonia , Phytophthora , Fusarium , and Pythium in legumes and other crops [ 16 ]. Actinobacteria, which have been traditionally known as actinomycetes, are a heterogeneous group of aerobic, filamentous and Gram-positive bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…The genus Streptomyces is represented in nature by the largest number of species among the family Actinomycetaceae [ 17 ]. This genus, as a colonizer of the rhizosphere, is able to: (i) act as BCA of plant pathogenic fungi, (ii) produce siderophores, (iii) produce plant growth promoting substances, (iv) promote nodulation, (v) produce biodegradative enzymes such as chitinases, cellulases, glucanases, peroxidases, and (vi) assist Rhizobium bacteria in iron assimilation, or in nitrogen fixation in legumes, which indirectly contributes to the promotion of plant growth [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

Díaz-Díaz

Bernal-Cabrera

Trapero

et al. 2022

Plants

View full text Add to dashboard Cite

Macrophomina phaseolina and Rhizoctonia solani are considered two major soil-borne pathogens of Phaseolus vulgaris in Cuba. Their management is difficult, not only due to their intrinsic biology as soil-borne pathogens, but also because the lack of active ingredients available against these pathogens. Actinobacteria, a heterogeneous bacterial group traditionally known as actinomycetes have been reported as promising biological control agents (BCAs) in crop protection. Thus, the main objective of this study was to evaluate the effectiveness of 60 actinobacterial strains as BCAs against M. phaseolina and R. solani in vitro by dual culture assays. The most effective strains were characterized according to their cellulolytic, chitinolytic and proteolytic extracellular enzymatic activity, as well as by their morphological and biochemical characters in vitro. Forty and 25 out of the 60 actinobacteria strains inhibited the mycelial growth of M. phaseolina and R. solani, respectively, and 18 of them showed a common effect against both pathogens. Significant differences were observed on their enzymatic and biochemical activity. The morphological and biochemical characters allow us to identify all our strains as species belonging to the genus Streptomyces. Streptomyces strains CBQ-EA-2 and CBQ-B-8 showed the highest effectiveness in vitro. Finally, the effect of seed treatments by both strains was also evaluated against M. phaseolina and R. solani infections in P. vulgaris cv. Quivicán seedlings. Treatments combining the two Streptomyces strains (CBQ-EA-2 + CBQ-B-8) were able to reduce significantly the disease severity for both pathogen infections in comparison with the non-treated and inoculated control. Moreover, they showed similar effect than that observed for Trichoderma harzianum A-34 and with Celest® Top 312 FS (Syngenta®; Basilea, Switzerland) treatments, which were included for comparative purposes.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

Díaz-Díaz

Bernal-Cabrera

Trapero

et al. 2022

Plants

View full text Add to dashboard Cite

show abstract

“…Streptomyces bacteria as biocontrol agents produced secondary metabolites such as antimicrobials volatile compounds, IAA hormone, and plant protection from biotic stresses, which colonize plant roots. They stimulate plant growth and control different bacterial and fungal pathogens (Vurukonda et al 2021). The last genus, with a frequency of 3.22%, belongs to the genus Exiguobacterium from the family of Bacillaceae.…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of endophytic bacteria associated with kiwifruit and their biocontrol potential against Meloidogyne incognita

Banihashemian

Jamali

Golmohammadi

et al. 2022

Egypt J Biol Pest Control

View full text Add to dashboard Cite

Background Root-knot nematodes (RKNs) cause severe losses in kiwifruit-growing regions. The endophytic bacteria could be biological agents for nematodes management. The aim of this study was the isolation and identification of endophytic bacteria from kiwifruit orchards and the evaluation of their antagonistic ability against RKN in greenhouse conditions. Results In this study, the population of nematode and the bacterial strains were isolated from kiwifruit roots and leaves in the Mazandaran and Guilan provinces of Iran. Molecular experiments were conducted to identify and confirm the bacterial isolates and RKN species. Also, the effects of bacterial isolates on nematode reproduction factors (number of galls, egg masses, and second-stage juveniles, J2) and growth parameters of kiwifruit plants were determined. The RKN was confirmed as Meloidogyne incognita by molecular identification. Also, the endophytic bacteria were identified based on supplementary experiments and molecular analyses. A total of 31 bacterial endophytes were identified to be including 12 genera of Bacillus, Pseudomonas, Staphylococcus, Exiguobacterium, Sphingomonas, Agrobacterium, Variovorax, Pantoea, Microbacterium, Streptomyces, Chryseobacterium, and Chitinophaga. Generally, Bacillus and Pseudomonas were the dominant genera that included 29.03 and 22.58% of total isolated bacteria, respectively. In vitro screening assays, P. ananatis 121.en and P. chlororaphis 54.en displayed considerable antagonistic ability on J2 mortality of M. incognita and were selected for greenhouse surveys. The isolates displayed a significant reduction in the number of galls and egg masses on roots and juvenile’s population in pot soil. Moreover, 121.en and 54.en strains significantly increased growth parameters including root fresh weight and shoot fresh weight than the control kiwifruit seedlings. Conclusions The bacterial endophytes are safe and have a low risk of managing the RKNs and can be effective microbial bio-fertilizers for improving kiwifruit plant growth under RKNs infections.

show abstract

“…Quite recently, considerable attention has been paid to the implementation of antagonistic microorganisms to suppress plant diseases engendered by phytopathogenic bacteria and fungi, with the purpose of avoiding the use of chemical control methods and overuse of fungicides (Suárez-Moreno et al, 2019). Actinobacteria and other bacterial genera such as Pseudomonas and Bacillus have been greatly investigated for their biocontrol potential (Vurukonda et al, 2021).…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

et al. 2023

View full text Add to dashboard Cite

Biocontrol is considered as an effective alternative to the application of agrochemicals, which are harmful to the environment, human, and animal health. In this study, twenty-six strains of actinomycetes were isolated from rhizospheric arid soil of the halophyte Atriplex halimus L. ῾Guettaf’ in Biskra province, Algeria. The six isolates that have inhibited at least three phytopathogenic fungi among the five tested (Fusarium oxysporum, Alternaria alternata, Fusarium solani, Aspergillus flavus and Botrytis cinerea) were selected, and have been tested in vitro against phytopathogenic bacteria (Pectobacterium carotovorum and Streptomyces scabies). They were also evaluated for their ability to hydrolyze phosphate, elaborate siderophores, produce indole-3-acetic acid (IAA), and to antagonize S. scabies in vivo (on radish seedlings). Based on the physicochemical analyses, soil samples were categorized as alkaline and extremely-saline. The antagonism results revealed varying antifungal potential among the selected isolates (Act11, Act16, Act17, Act18, Act23 and Act24), about 50% were able to inhibit the growth of F. solani and A. flavus, followed by 33.33% of those having antagonized F. oxysporum, while A. alternata was found to be the most sensitive. Only Act18 has antagonized S. scabies in vitro with an inhibition diameter zone of 19 ± 0.41 mm. However, in vivo trials showed that four isolates have counteracted S. scabies. Among them, Act18 and Act24 have significantly and positively affected the root surface (P = 0.0062) and prevented common scab. IAA was detected in all selected isolates with Act24 being the highest producer (77.45 μg mL−1). Additionally, degradation ability revealed that four isolates were able to hydrolyze phosphate while three exhibited the capacity of elaborating siderophores. The six isolates were assigned to Streptomyces genius according to their morphological, physiological and chemotaxonomical traits. Based on this study, Streptomyces sp. Act18 and Streptomyces sp. Act24 that tolerate 7.5% NaCl concentration, prevent common scab and exhibit some plant growth attributes, may be considered as promising biocontrol agents to be applied in arid and saline soils.

show abstract

Growth Promotion and Biocontrol Activity of Endophytic Streptomyces spp.

Cited by 5 publications

References 96 publications

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

Characterization of Actinobacterial Strains as Potential Biocontrol Agents against Macrophomina phaseolina and Rhizoctonia solani, the Main Soil-Borne Pathogens of Phaseolus vulgaris in Cuba

Isolation and identification of endophytic bacteria associated with kiwifruit and their biocontrol potential against Meloidogyne incognita

Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.

Contact Info

Product

Resources

About