Growth-promoting activity of indigenous Trichoderma isolates on wheat seed germination, seedling growth and yield

Abstract

Apart from their biocontrol potential, Trichoderma rhizosphere-competent strains stimulate plants growth via several direct mechanisms that affect various growth parameters. In this study, effect of five indigenous isolates (T. harzianum T 969, T. harzianum T 447, T. hamatum T 614, Trichoderma sp. isolate T and Gliocladium virens G525.) on wheat (variety Moghan 3) seed germination, seedling vigor and plant growth was examined through seeds treatment by the conidia and culture filtrate of the isolates. In the field experiments various growth parameters including rootlet and shoot length, stem height and weight, root length and weight, total leaf area, head and tiller number and 1000 grain weight as well as the plant total chlorophyll content and stomata conductance response were evaluated. In overall, seed germination rate was increased. The highest seed germination rate (95.8%) was observed for T. harzianum T969 non-sterilized cultural filtrate treatment and after 96 hours. The isolate G. virens G525 spore treatment decreased the rate by 54.5%. Although all isolates colonized on rootlets but no significant effect on the seedlings growth was observed. Isolate T. hamatum T614 caused necrotic reaction on rootlets, revealing its parasitic rather than symbiotic activity. In the field experiments, the isolates had significant effect on parameters such as stomata conductance, 1000 grain weight, ear fresh and dry weights, ear length and stem and root dry weight but they showed no significant effect on other evaluated parameters. Some isolates improved the affected parameters and some had negative effects which are discussed.

Impact of Soil Chemical Properties on the Growth Promotion Ability of Trichoderma ghanense, T. tomentosum and Their Complex on Rye in Different Land-Use Systems

“…In our experiment, the highest increase in the total weight of early rye seedlings was 19.3% (p = 0.014). However, our other findings were consistent with observations by Hajieghrari and Mohammadi [51] that some Trichoderma strains capable of colonizing roots showed no significant effect on wheat seedling parameters. Moreover, our results showed that the shoots and roots of the rye seedlings did not always correlate with seedling dry weight.…”

Trichoderma koningiopsis applied as seed coating protects sweet sorghum (Sorghum bicolor (L.) Moench) from fungal contaminants during storage

“…Results on seeds of other cereal species such as corn that were treated with Trichoderma extracts, showed a reduction in germination rate, but not in the nal rate of seed germination. However, when seeds were treated with the spore suspension the germination was inhibited due to the colonization of the seed surface by the microorganism (Hajieghrari, B., 2010). The opposite occurs with chili and soybean seeds treated with different strains of Trichoderma, which showed a positive effect on germination rates (Mukhtar et al 2012).…”

Niche differentiation of microbes and their functional signatures in Assam type tea (Camellia sinensis var. assamica)

“…Trichoderma can colonize the intracellular spaces, as well as the spaces between the plasma membrane and the cell wall of the root tissue (Ramírez-Valdespino et al 2019). Once in the endosphere, the fungus can promote seed germination, development of root, shoot and biomass, increase tiller number and overall crop yield (Hajieghrari and Mohammadi 2016). Trichoderma can not only modulate levels of auxin through a crosstalk with MAP-kinases based signaling pathway but also contributes its own hormone or hormone intermediates thereby synergistically in uencing the root architecture (Viterbo et al 2005;Brotman et al 2013).…”