Growth performance, vitamin E status, and proximate and fatty acid composition of channel catfish, Ictalurus punctatus, fed diets containing various levels of fish oil and vitamin E

Abstract

A study was conducted to determine the effect of increasing dietary levels of fish oil on vitamin E requirement and their effect on growth performance, liver vitamin E status, and tissue proximate and fatty acid compositions of channel catfish. Basal purified diets (42% protein and 3,800 kcal DE/kg) supplemented with 6, 10, and 14% menhaden fish oil were each supplemented with 50, 100, and 200 mg vitamin E/kg (3×3 factorial experiment). Each diet was fed to juvenile channel catfish in three random aquaria to apparent satiation twice daily for 12 weeks. Weight gain, feed intake, and feed efficiency ratio were not affected by dietary levels of fish oil, vitamin E, or their interaction. Survival rate at the end of week 12 was significantly lower for fish fed diets containing 14% fish oil, regardless of vitamin E content. Whole-body moisture significantly decreased and lipid increased when dietary lipid levels were increased to 10 or 14%. Dietary vitamin E levels had no effect on body proximate composition. Lipid content of liver was not influenced by dietary levels of fish oil and vitamin E or their interaction. Hepatosomatic index significantly decreased with increasing lipid levels but was not affected by dietary levels of vitamin E. Liver vitamin E increased with increasing dietary vitamin E but decreased with increasing fish oil levels. Fatty acid composition of whole body and liver reflected that of dietary lipid but was not influenced by dietary levels of vitamin E. Whole-body saturates increased, whereas MUFA decreased with increasing dietary levels of fish oil. Liver saturates were not affected by fish oil levels, but MUFA and n-6 decreased and increased, respectively, with increasing fish oil levels. Total n-3 and n-3 HUFA in both tissues increased with increasing fish oil levels in diets, but liver stored much higher levels of these fatty acids.

High-fat diet reduces local myostatin-1 paralog expression and alters skeletal muscle lipid content in rainbow trout, Oncorhynchus mykiss

“…As with glucose concentrations, lower hepatosomatic indices (HSI) were detected in HFD-fed fish. Menhaden oil has been shown to lower HSI in channel catfish (Lim et al 2010); however, the reason for lower HSI in our or the Lim study is not clear. Lim and coworkers did not identify differences in final masses between treatment groups, a finding our data support.…”

Molecular characterization, phylogenetic analysis and expression patterns of five protein arginine methyltransferase genes of channel catfish, Ictalurus punctatus (Rafinesque)

“…Channel catfish (NWAC 103 strain, were raised and acclimated at 28°C for 2 weeks before use according to the protocol established in the USDA ARS Aquatic Animal Health Research Unit (Lim et al 2009). The Institutional Animal Care and Use Committee of the USDA ARS Aquatic Animal Health Research Unit approved the animal usage in experiments.…”

Influence of dietary vitamin E (DL‐alpha‐tocopheryl acetate) and diludine (diethyl 1,4‐dihydro‐2,6‐dimethyl‐3,5‐pyridinedicarboxylate) levels on growth and lipid peroxidation in rainbow trout, Oncorhynchus mykiss

“…After 10-week feeding trial, whole body lipid content was not significantly affected by the dietary vitamin E and diludine. Likewise, dietary vitamin E did not cause any difference in whole body lipid content in meagre (Rodríguez Lozano et al, 2017) and channel catfish, Ictalurus punctatus (Lim et al, 2010). On the other hand, dietary vitamin E increased the lipid level in silver pomfret (Pampus argenteus) body at 50 mg/kg dietary level but there was no effect when fish was fed at 100 mg/kg of dietary vitamin E (Hossain et al, 2016).…”