Growth performance, nutrient digestibility, antioxidant state, ileal histomorphometry, and cecal ecology of broilers fed on fermented canola meal with and without exogenous enzymes

Abstract

This study was conducted to evaluate the effects of supplementation of exogenous enzymes in broiler diets that includes fermented canola meal on performance, nutrient digestibility, biochemical indication, antioxidative capacity, digestive enzyme activity, immune responses, and gut health. Five hundred 1-day-old Ross 308 broiler chicks were randomly allocated into five experimental groups (5 replicate/group), the first group: a control (CON) contained a basal diet, and the second to the fifth groups were fed diets as follows: containing 20% canola meal (CM), contains 20% fermented canola meal (FCM), contains 20% canola meal and exogenous enzymes at 0.02%/kg feed (ECM), and contains 20% fermented canola meal and exogenous enzymes at 0.02%/kg feed (EFC), respectively. At the finisher phase, the best body weight gain, feed conversion ratio, and nutrient utilization were associated with chickens fed EFC compared to other groups (P < 0.05). Total protein, albumin, alanine aminotransferase, and superoxide dismutase levels increased (P < 0.05), while cholesterol and malondialdehyde levels decreased in chickens fed on EFC. Likewise, there was a significant increase in the relative weight of the bursa of Fabricius and antibody titer against Newcastle disease, whereas the weight of abdominal fat decreased in the EFC group compared to other groups. Furthermore, there was a significant improvement in the activity of lipase and amylase enzymes (P < 0.05) in the EFC group. Fermented canola meal addition improved gut health (decreased Escherichia coli, increased Lactobacillus, and the highest values of villus height). Overall, these results confirmed that supplementing a fermented canola meal diet with exogenous enzymes improved growth performance through enhancing nutrient digestibility, immunity, antioxidant capacity, and gut health. Thus, adding enzymes to a diet containing fermented canola meal can be recommended as an alternative protein source that could be safely used to replace up to 20% soybean meal in broiler diets.

Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens

“…Compared with a corn-based diet, adding carbohydrases to barley during fermentation enhanced the activities of starch digestive enzymes (amylase, maltase and sucrase), thus improving the metabolizability of barley-based diets for broilers, which agrees with the findings of Perera et al [26,57]. As such, the fermentation process can degrade macromolecular factors and anti-nutritional elements in raw feed stuff via microorganisms, thereby facilitating the feed's metabolizability and absorption, with an improvement in the subsequent growth performance of the broilers [58]. Furthermore, applying feed fermentation technology to broiler feed not only decreases anti-nutritional substances, but also augments the concentrations of beneficial probiotic bacteria, enzymes and fermentation metabolites [59,60].…”

The effects of fermented feedstuff derived from Citri Sarcodactylis Fructus by-products on growth performance, intestinal digestive enzyme activity, nutrient utilization, meat quality, gut microbiota, and metabolites of broiler chicken

“…Research findings indicate that the incorporation of probiotics into fermented feed exerts favorable impacts on animal physiology, enhancing nutrient digestion and assimilation while minimizing feed resource wastage ( 20 , 21 ). Research has indicated that supplementing fermented feed can increase the final weight of piglets on day 42, as well as increase their ADG and ADFI, while decreasing their feed conversion ratio (F/G) ( 22 ).…”

Effect of fermented rapeseed meal on growth performance, nutrient digestibility, and intestinal health in growing pigs

“…A balanced gut flora is beneficial for the proper function of the digestive system ( Song et al., 2010 ). Previous studies reported that fermented diets improved gastrointestinal function, decreased counts of E. coli and increased the counts of Lactobacillus ( Elbaz et al., 2023 ; Grela et al., 2018 , 2022). This was confirmed in this experiment.…”