Growth of various strains of sulfate-reducing bacteria of human large intestine

Kushkevych, Ivan; Moroz, O. M.; Halytsky, Danylo

doi:10.30970/sbi.0603.243

Cited by 11 publications

(14 citation statements)

References 18 publications

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“…In previous research, the author has studied the ultrastructure of cells of the isolated bacteria under the electron microscope [17]. In the photos it was visible that the cells were rodshaped or vibrioshaped, surrounded by a cell wall and cytoplasmic membrane.…”

Section: Resultsmentioning

confidence: 99%

Identification of sulfate-reducing bacteria strains of human large intestine

Kushkevych

2013

Biol. Stud.

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The sulfate-reducing bacteria have been isolated from human intestine. These bacteria are identified by their morphological, physiological and biochemical characteristics as the Desulfovibrio sp. and Desulfomicrobium sp. according to Bergey's Manual of Determinative Bacteriology. Growth of the isolated strains of bacteria has been studied under various conditions including types of media, effect of oxygen, pH, and tempe rature. Bacterial growth in the modified KravtsovSorokin's medium, and process of dissimilatory sulfate reduction by the selected strains of bacteria have been investiga ted. All obtained bacterial cultures used sulfate and lactate intensively as well as produced hydrogen sulfide and acetate in the medium. The Desulfovibrio sp. strain Vib7 produced the highest concentration (up to 3.23 mM) of hydrogen sulfide, while the bacteria used about 99% of sulfate ions presented in the medium. This strain also produced the highest concentration of acetate ions (up to 15.87 mM) on the fifth day of cultivation. Among the studied Desulfomicrobium sp. strains, the highest concentration of hydrogen sulfide was produced by the Desulfomicrobium sp. strain Rod9, and the highest concentration of acetate ions was produced by the Desulfomicrobium sp. strain Rod4.

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Section: Resultsmentioning

confidence: 99%

Identification of sulfate-reducing bacteria strains of human large intestine

Kushkevych

2013

Biol. Stud.

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“…strains (SRB Rod-1, SRB Rod-2, SRB Rod-3, SRB Rod-4, SRB Rod-5, SRB Rod-6, SRB Rod-7, SRB Rod-8, SRB Rod-9, SRB Rod-10) isolated from the human large intestine [17]. The strains are kept in the collection of microorganisms at the Biotechnology Laboratory of the Pharmacy Faculty at the University of Veterinary and Pharmaceutical Sciences Brno (Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

Growth of the Desulfomicrobium sp. strains, their sulfate- and lactate usage, production of sulfide and acetate by the strains isolated from the human large intestine

Kushkevych¹

2014

Microbiol Discov

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The aim and background of the study were to investigate bacterial growth of the Desulfomicrobium sp. strains from human large intestine, and to study the processes usage of sulfate-and lactate, production of sulfide and acetate by the obtained bacteria as well as to carry out cluster and correlation analysis of these processes. Microbiology methods of the study for bacterial strains cultivation and photometric methods for determination of bacterial biomass and hydrogen sulfide concentration were used, sulfate ions concentration was determined by turbidymetric method, lactate concentration was carried out by lactate dehydrogenase. Acetate ions accumulation by the strains was determined by titration. Using the experimental data, the methods of statistical analysis have been also used. The highest biomass (3.34 g/l) has been accumulated by the Desulfomicrobium sp. strain Rod-9 on the sixth day of cultivation, in comparison to the other strains. Studied strains of the Desulfomicrobium sp. actively reduced the sulfate ions and produced hydrogen sulfide. The highest concentration of hydrogen sulfide (3.14 mM) was produced by the SRB Rod-9 strain on the eighth day of cultivation; while, the strain used about 98% of the sulfate compared with its initial concentration. The strains used lactate fully on the sixth day of cultivation and produced acetate. Clustering of the parameters of the bacterial growth, the sulfate and lactate usage as well as hydrogen sulfide and acetate production by the various Desulfomicrobium sp. strains has been carried out. The correlation coefficients between the parameters have been determined.

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“…strains (SRB Vib-1, SRB Vib-2, SRB Vib-3, SRB Vib-4, SRB Vib-5, SRB Vib-6, SRB Vib-7, SRB Vib-8, SRB Vib-9, SRB Vib-10) obtained from the human large intestine [11].…”

Section: Materіal and Methodsmentioning

confidence: 99%