Growth of uropathogenic Escherichia coli strains at solid surfaces

Harkes, G.; Dankert, J.; Feijén, Jan

doi:10.1163/156856292x00213

Cited by 33 publications

(15 citation statements)

References 34 publications

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“…A positive correlation between substratum hydrophobicity and the detachment of adherent biofilm was established by other studies. According to this approach, bacteria attached to hydrophobic materials were more easily removed from them (Harkes et al, 1992; Reid et al, 1993; Eginton et al, 1995; Bos et al, 2000; Gómez-Suárez et al, 2001). On the other hand, S. aureus found to attach more effectively on stainless steel surfaces in comparison with glass ones, while organosilanes enhance the adherence of bacterium to modified glass surfaces.…”

Section: Discussionmentioning

confidence: 99%

Anti-adhesion and Anti-biofilm Potential of Organosilane Nanoparticles against Foodborne Pathogens

et al. 2017

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Nowadays, modification of surfaces by nanoparticulate coatings is a simple process that may have applications in reducing the prevalence of bacterial cells both on medical devices and food processing surfaces. To this direction, biofilm biological cycle of Salmonella Typhimurium, Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus, and Yersinia enterocolitica on stainless steel and glass surfaces, with or without nanocoating was monitored. To achieve this, four different commercial nanoparticle compounds (two for each surface) based on organo-functionalized silanes were selected. In total 10 strains of above species (two for each species) were selected to form biofilms on modified or not, stainless steel or glass surfaces, incubated at 37°C for 72 h. Biofilm population was enumerated by bead vortexing-plate counting method at four time intervals (3, 24, 48, and 72 h). Organosilane based products seemed to affect bacterial attachment on the inert surfaces and/or subsequent biofilm formation, but it was highly dependent on the species and material of surfaces involved. Specifically, reduced bacterial adhesion (at 3 h) of Salmonella and E. coli was observed (P < 0.05) in nanocoating glass surfaces in comparison with the control ones. Moreover, fewer Salmonella and Yersinia biofilm cells were enumerated on stainless steel coupons coated with organosilanes, than on non-coated surfaces at 24 h (P < 0.05). This study gives an insight to the efficacy of organosilanes based coatings against biofilm formation of foodborne pathogens, however, further studies are needed to better understand the impact of surface modification and the underlying mechanisms which are involved in this phenomenon.

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Section: Discussionmentioning

confidence: 99%

Anti-adhesion and Anti-biofilm Potential of Organosilane Nanoparticles against Foodborne Pathogens

et al. 2017

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“…For example, a variety of virulence factors in E. coli , such as hemolysin, fimbriae, lipopolysaccharide (LPS), secreted proteins, capsules and iron-acquisition systems [11], [12], which allow bacterial colonization in the mucosal epithelial cells lining the urogenital tract, invade, and further form intracellular biofilm-like pods in uroepithelial cells [13]. Remarkably, UPEC is capable of forming biofilms on the abiotic surfaces of indwelling medical devices such as catheters [14], [15], which lead to “Catheter-Associated Urinary Tract Infection” (CAUTI) in clinics [16]. CAUTI is especially fatal in immunocompromised, debilitated and diabetic patients [17], [18].…”

Section: Introductionmentioning

confidence: 99%

Pleiotropic Roles of uvrY on Biofilm Formation, Motility and Virulence in Uropathogenic Escherichia coli CFT073

et al. 2013

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Urinary tract infections primarily caused by uropathogenic strains of Escherichia coli (E. coli) remain a significant public health problem in both developed and developing countries. An important virulence determinant in uropathogenesis is biofilm formation which requires expression of fimbriae, flagella, and other surface components such as lipopolysaccharides. In this study, we explored the regulation of uvrY and csrA genes in biofilm formation, motility and virulence determinants in uropathogenic E. coli. We found that mutation in uvrY suppressed biofilm formation on abiotic surfaces such as polyvinyl chloride, polystyrene and glass, and complementation of uvrY in the mutant restored the biofilm phenotype. We further evaluated the role of uvrY gene in expression of type 1 fimbriae, an important adhesin that facilitates adhesion to various abiotic surfaces. We found that phase variation of type 1 fimbriae between fimbriated and afimbriated mode was modulated by uvrY at its transcriptional level. Deletion mutant of uvrY lowered expression of fimbrial recombinase genes, such as fimB, fimE, and fimA, a gene encoding major fimbrial subunit. Furthermore, transcription of virulence specific genes such as papA, hlyB and galU was also reduced in the deletion mutant. Swarming motility and expression of flhD and flhC was also diminished in the mutant. Taken together, our findings unravel a possible mechanism in which uvrY facilitates biofilm formation, persistence and virulence of uropathogenic E. coli.

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“…have reported that the use of cationic aluminium ions affected the surface negativity of the root cell membranes, which resulted in the destabilization of the membranes. Harkes et al . witnessed the absence of growth of microorganisms (E. coli) on the positively charged poly(methylmethacrylate) films.…”

Section: Resultsmentioning

confidence: 99%

Dopant Variation as a Probe into the Antimicrobial activity of Polyaniline/Carbon nanofiber/Poly(methylmethacrylate) Composite

Anju

Narayanankutty

2018

ChemistrySelect

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Polymeric composites based on polymethylmethacrylate (PMMA), carbon nanofiber (CNF) and polyaniline (PANI) were prepared and the effect of various dopants on the morphology and antibacterial property was evaluated. CNF was given a coating of PANI through in situ polymerization method by adding five different acid dopants (hydrochloric acid (HCl), sulphuric acid (H 2 SO 4 ), phosphoric acid (H 3 PO 4 ), toluene sulphonic acid (TSA) and camphor sulphonic acid (CSA)) and was used to prepare the composite by solution casting method. The composites were characterized by X-ray diffraction, FTIR spectroscopy, BET surface area analysis, DLS measurements, SEM and TEM microscopic techniques. Scanning electron micrographs and surface area analysis showed that phosphoric acid doped composite had the smallest average size and highest surface area among other four composites. The activity of the composites against Escherichia coli, Klebsiella pneumoniae and Bacillus subtilis bacteria was evaluated by agar well diffusion method. The antibacterial activity was assessed by measuring the inhibition zone diameter and minimum inhibitory concentration. The composites showed variable toxicity indicating a dependence on the type of doping agent used. A zeta potential value of + 39.60 mV was obtained for phosphoric acid doped PANI/CNF composites, indicating its antibacterial property. The results showed that the antibacterial activity of this composites is tunable.[a] Dr.

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Growth of uropathogenic Escherichia coli strains at solid surfaces

Cited by 33 publications

References 34 publications

Anti-adhesion and Anti-biofilm Potential of Organosilane Nanoparticles against Foodborne Pathogens

Anti-adhesion and Anti-biofilm Potential of Organosilane Nanoparticles against Foodborne Pathogens

Pleiotropic Roles of uvrY on Biofilm Formation, Motility and Virulence in Uropathogenic Escherichia coli CFT073

Dopant Variation as a Probe into the Antimicrobial activity of Polyaniline/Carbon nanofiber/Poly(methylmethacrylate) Composite

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