Growth of Trametes versicolor on phenol

Abstract

Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain utilized completely 0.5 g/l phenol in 6 days. The dynamics of the phenol degradation process was investigated. The process was characterized by specific growth rate micromax 0.33 h(-1), metabolic coefficient k=4.4, yield coefficient Yx/s=0.23 and rate of degradation Q=0.506 h(-1). The intracellular activities of phenol hydroxylase (0.333 U/mg protein) and cis,cis-muconate lactonizing enzyme (0.41 U/mg protein) were demonstrated for the first time in this fungus. In an attempt to estimate the occurrence of gene sequences in T. versicolor 1 related to phenol degradation pathway a dot blot analysis with total DNA isolated from this strain was performed. Two synthetic oligonucleotides were used as hybridizing probes. One of the probes was homologous to the 5'end of phyA gene coding for phenol hydroxylase in Trichosporon cutaneum ATCC 46490. The other probe was created on the basis of cis,cis-muconate lactonizing enzyme coding gene in T. cutaneum ATCC 58094. The results of these investigations showed that T. versicolor 1 may carry genes similar to those of Trichosporon cutaneum capable to degrade phenol.

Comparison of the Biodegradation Capacity of Two Fungal Strains Toward a Mixture of Phenol and Cresol by Mathematical Modeling

“…Samples were taken at 24 h intervals and cells were pelleted by centrigugation at 5000 rpm for 20 min. the dry weight of the cells was determined by an UltRA X apparatus for drying (8). the content of phenols in the supernatant was determined by hPlc analyses performed in a c 18 10 μm Bondapac column (3.9 mm × 300 mm) and Waters 484 UV detector (260 nm).…”

“…the degradation potential of Aspergillus awamori and Trametes versicolor toward phenol, phenol derivatives and their mixtures was analyzed in our previous investigations (7,8,9). it was obtained that the removal or degradation of one or all components could be slowed down and/or stopped depending on the composition of the studied mixture.…”

Degradation of Mixed Aromatic Pollutants by Trametes Versicolor Strain 1

“…The capacity of Trametes versicolor 1 to utilize phenol was previously demonstrated (9). It was shown that the strain had mineralized phenol (0.5 g/l).…”

“…It was shown that the strain had mineralized phenol (0.5 g/l). (9). These results gave us a reason to investigate the strain ability to degrade a model mixture of phenolic industrial pollutants.…”

“…Biodegradation was conducted on the carbon-free CzapekDox medium containing phenolic compounds as sole carbon and energy sources (9) The flasks containing 50 ml of inoculated culture medium were agitated on a New Brunswick rotary shaker (240 rpm) at 28 °C. Samples were taken at a 24 h interval and centrifuged at 5000 rpm for 20 min to settle the cells down.…”