Growth of Mumps Virus in Organ Cultures of Rhesus Testis

Bigazzi, P.L.; Barron, Almen L.; Flanagan, Thomas D.; Andrada, J A; Witebsky, Ernest

doi:10.1093/infdis/118.4.411

Cited by 7 publications

(3 citation statements)

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“…Among them, the mumps virus is well known for its testicular tropism and for inducing inflammation, decreased androgen production and degeneration of the seminiferous epithelium that can lead to sterility (reviewed in Dejucq and Jegou, 2001 ). As shown in the testicular interstitial tissue in vivo ( Bigazzi et al ., 1968 ; Bjorvatn, 1973 ), the mumps virus has been found to replicate in Leydig cells and be associated with a decrease in testosterone production in vitro ( Le Goffic et al ., 2003 ). Interestingly, the latter study reported that mumps virus infection of human Leydig cells did not seem to induce IFN production by these cells ( Le Goffic et al ., 2003 ), in contrast to their rat counterpart exposed to another paramyxoviridae virus, the Sendai virus ( Dejucq et al ., 1998 ).…”

Section: Introductionmentioning

confidence: 72%

Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation

et al. 2008

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BACKGROUNDThe immuno-privileged status of the testis is essential to the maintenance of its functions, and innate immunity is likely to play a key role in limiting harmful viral infections, as demonstrated in the rat. In men mumps virus infects Leydig cells and has deleterious effects on testosterone production and spermatogenesis. The aim of this study was to test whether mumps virus infection of isolated human Leydig cells was associated with an inhibition of their innate antiviral defences.METHODSLeydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins—2′5′ oligoadenylate synthetase (2′5′OAS), double-stranded RNA-activated protein kinase (PKR) and MxA—was investigated, in the absence or presence of mumps virus or viral stimuli including poly I:C, a mimetic of RNA viruses replication product.RESULTSStimulated or not, human Leydig cells appeared unable to produce routinely detectable IFNs α, β and γ. Although the level of PKR remained unchanged after stimulation, the expression of 2′5′OAS and MxA was enhanced following either mumps virus or poly I:C exposure (P < 0.05 versus control).CONCLUSIONSOverall, our results demonstrate that mumps virus replication in human Leydig cells is not associated with a specific inhibition of IFNs or 2′5′OAS, MxA and PKR production and that these cells display relatively weak endogenous antiviral abilities, as opposed to their rat counterparts.

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Section: Introductionmentioning

confidence: 72%

Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation

et al. 2008

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“…Such a dysfunction would also account for the higher incidence of testicular cancer in men who have had mumps than in control men (34). Two publications suggested that mumps virus replicates within the testis: Bigazzi et al (42,43) showed viral replication in the interstitial tissue in organotypic culture of monkey testis following virus inoculation through the testicular vein, and Bjorvatn et al (44) rescued mumps virus by needle aspiration of testis biopsy specimens from mumps-infected patients. Two studies (113,177) showed that systemic treatment with interferon (IFN) prevented the testicular atrophy caused by mumps orchitis in all treated patients (4 of 4 and 13 of 13, respectively).…”

Section: The Human Testismentioning

confidence: 99%

Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System

Dejucq

Jégou

2001

Microbiol Mol Biol Rev

287

220

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This review describes the various viruses identified in the semen and reproductive tracts of mammals (including humans), their distribution in tissues and fluids, their possible cell targets, and the functional consequences of their infectivity on the reproductive and endocrine systems. The consequences of these viral infections on the reproductive tract and semen can be extremely serious in terms of organ integrity, development of pathological and cancerous processes, and transmission of diseases. Furthermore, of essential importance is the fact that viral infection of the testicular cells may result not only in changes in testicular function, a serious risk for the fertility and general health of the individual (such as a fall in testosteronemia leading to cachexia), but also in the possible transmission of virus-induced mutations to subsequent generations. In addition to providing an exhaustive account of the data available in these domains, this review focuses attention on the fact that the interface between endocrinology and virology has so far been poorly explored, particularly when major health, social and economical problems are posed. Our conclusions highlight the research strategies that need to be developed. Progress in all these domains is essential for the development of new treatment strategies to eradicate viruses and to correct the virus-induced dysfunction of the endocrine system

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“…This report documents characteristics of plaque formation by various mumps virus strains as well as a plaque reduction neutralization procedure for assay of mumps antibody. The system used has been previously reported (1,4).…”

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confidence: 99%

Plaque Formation by Mumps Virus and Inhibition by Antiserum

Flanagan¹,

Barron²

1970

Applied Microbiology

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Boston and ABC strains of mumps virus produced plaques approximately 1.0 mm in diameter in monolayers of BGM cells. The plaques were circular and either clear or target-like in form. Ricki strain virus produced plaques of similar size and form but, in addition, a red plaque was observed with this agent. The vaccine strain of mumps virus, Jeryl Lynn, produced minute clear plaques approximately 0.3 mm in diameter. Incorporation of diethylaminoethyl (DEAE)-dextran into the overlay medium did not affect the size difference between Jeryl Lynn plaques and those of the other strains. However plaques of the Jeryl Lynn and Ricki strains were more easily visualized when the overlay medium contained 400 ug/ml of DEAE-dextran.

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Growth of Mumps Virus in Organ Cultures of Rhesus Testis

Cited by 7 publications

References 0 publications

Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation

Antiviral responses of human Leydig cells to mumps virus infection or poly I:C stimulation

Viruses in the Mammalian Male Genital Tract and Their Effects on the Reproductive System

Plaque Formation by Mumps Virus and Inhibition by Antiserum

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