Growth of Measles Virus in Various Human Lymphoid Cell Lines

Minagawa, Tomonori; Sakuma, Takashi

doi:10.1111/j.1348-0421.1977.tb02804.x

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…Occasionally the virus is able to establish a persistent infection, best described in subacute sclerosing panencephalitis. Persistent infection of lymphoid cell lines has also been reported 31,32 and the virus has been detected in peripheral blood mononuclear cells and lymphoid subsets. [33][34][35] These data, coupled with the case reports mentioned above, suggest that MV may be able to infect and persist in HRS cells.…”

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confidence: 88%

Measles virus and classical Hodgkin lymphoma: No evidence for a direct association

Wilson

Freeland

Gallagher

et al. 2007

Intl Journal of Cancer

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A proportion of Hodgkin lymphoma (HL) cases are causally associated with the Epstein-Barr virus (EBV) but the aetiology of the remaining cases remains obscure. Over the last 3 decades several studies have found an association between HL and measles virus (MV) including a recent cohort study describing the detection of MV antigens in Hodgkin and Reed-Sternberg cells, the tumour cells in HL. In the present study we looked at the relationship between history of MV infection and risk of developing HL in a population-based, case/control study of HL. In addition we used immunohistochemistry and RT-PCR to look for direct evidence of MV in HL biopsies. There was no significant difference in the proportion of cases reporting previous measles compared to controls in the entire data set or when young adults were considered separately. Using a robust immunohistochemical assay for MV infection, we failed to find evidence of MV in biopsies from 97 cases of HL and RT-PCR studies similarly gave negative results. This study therefore provides no evidence that MV is directly involved in the development of HL. However, when age at first reported MV infection was investigated, significant differences emerged with children infected before school-age having higher risk, especially of EBV2ve HL, when compared with children infected at older ages; the interpretation of these latter results is unclear. ' 2007 Wiley-Liss, Inc.Key words: Hodgkin lymphoma; measles virus; PCR; epidemiology The term Hodgkin lymphoma (HL) encompasses 2 entities, nodular lymphocyte predominant HL and classical HL (cHL), which are now recognised as distinct disease processes.1 The pathology and epidemiology of cHL, which accounts for almost 95% of cases, also suggest that this is a heterogeneous entity that is likely to have more than one aetiology.2 Histologically, cHL is divided into 4 subtypes on the basis of the appearance of the rare tumour cells, the Hodgkin and Reed-Sternberg (HRS) cells, and the composition of the more plentiful background infiltrate.3 The most common subtypes are nodular sclerosis (NSHL) and mixed cellularity (MCHL).3 cHL generally shows a bimodal age-specific incidence curve but there is marked geographical variation in the shape of the curve and the age at which the incidence peaks occur. 4,5 It is the most common lymphoma in young adults in the Western world and the most common childhood malignancy in some developing countries.6,7 Epstein-Barr virus (EBV) is present in the HRS cells in a proportion of cHL cases and is believed to contribute to disease causation.8 EBV-associated cases (EBV1ve cHL) are not evenly distributed across all patient subgroups; cases of the MCHL subtype are more likely to be EBV-associated than NSHL cases, as are cases diagnosed in early childhood (<10 years) and older adulthood (>50 years).2 Consistent with this, a higher proportion of cases in developing countries are EBV-associated compared to developed countries, in which around 33% of cHL cases are EBV-positive. 9,10An increased risk of developing young...

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confidence: 88%

Measles virus and classical Hodgkin lymphoma: No evidence for a direct association

Wilson

Freeland

Gallagher

et al. 2007

Intl Journal of Cancer

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“…33 YAMANO UCHI (CNS) leads to measles encephalitis in rare cases. In vitro studies indicate that MV can grow in lymphoblastoid cell lines of both T and B cell types (Joseph, Lampert and Oldstone, 1975;Sullivan et al, 1975;Barry et al, 1976;Minagawa et al, 1976;Minagawa and Sakuma, 1977). In small lymphocytes, MV is thought to exist in a silent state that can be activated to pro duce mature virus in lymphoblastoid cells (Osunkoya et al, 1974a,b;Lucas et al, 1978).…”

Section: Pathogenesis Of MV Infectionmentioning

confidence: 99%

Comparative Aspects of Pathogenicity of Measles, Canine Distemper, and Rinderpest Viruses

Yamanouchi¹

1980

JJMSB

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Nuclear and Cytoplasmic Immunofluorescence in the Cerebrospinal Fluid Cells from a Neonate with Congenital Rubella

Nunoue

Goya

1977

Microbiology and Immunology

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It is known that rubella virus is isolated from the cerebrospinal fluid (CSF) of young infants with congenital rubella. (1, 3, 5, 7) On the contrary it is difficult to isolate the virus from the CSF of patients with acquired rubella encephalitis. We examined the cells in the CSF from a neonate with congenital rubella syndrome, in comparison with the cells of acquired rubella, by the immunofluorescence method.A case, M.T., male. His mother had rubelliform rash on the 38th day of gestation. The baby was born 11 days later than the expected date, with a weight of 2,100 g, microcephaly, cataracts, suggestive of congenital heart disease, left inguinal hernia and probably deafness which became distinct later. Titers of hemagglutination inhibition (HI) antibody in the baby's serum and in his mother's were 1 :256 and 1:512, respectively. Specific IgM antibody was contained only in the former. On the 23rd day of age, spinal tap was done, because of suspicious signs of intracranial involvement, and a small amount of CSF was obtained, in which 67 lymphocytes and 3 polymorphonuclear cells were contained per cubic millimeter.As controls were examined CSF cells of 15 cases with central nervous system (CNS) complication of acquired rubella and of 16 cases with CNS diseases without rubella infection.The CSF was centrifuged at 1,500 rpm for 15 min. The sediment was washed once with 10 ml of PBS, pH 7.2, then smeared on cover-slips, dried in room temperature and fixed in cold aceton at -20 C for 15 min. An indirect immunofluorescent technique was applied with a hyperimmune rabbit serum against rubella virus M33 (supplied by Dr. J. Nonaka, the Chemo-Sero-Therapeutic Research Institute) and a goat anti-rabbit gamma-globulin conjugated with fluorescein isothiocyanate (FITC) (Behringwerke). The rabbit serum whose HI antibody titer was 1 :10,240, was diluted to 1 :500 after absorption with bovine liver powder (NBC). A blocking test to testify its specificity was done with human convalescent serum with an HI antibody titer of 1:256, including IgG and IgM antibodies. Specificity of immunofluorescence was repeatedly examined also with rubella virus-infected BHK-21 and Vero cells (6).The specific immunofluorescence observed was diffuse in the cytoplasm and granular in the nucleus of lymphocytes derived from the CSF of the neonate with congenital rubella. Only cytoplasmic fluorescence was seen in lymphocytes of the 731

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Growth of Measles Virus in Various Human Lymphoid Cell Lines

Cited by 3 publications

References 9 publications

Measles virus and classical Hodgkin lymphoma: No evidence for a direct association

Measles virus and classical Hodgkin lymphoma: No evidence for a direct association

Comparative Aspects of Pathogenicity of Measles, Canine Distemper, and Rinderpest Viruses

Nuclear and Cytoplasmic Immunofluorescence in the Cerebrospinal Fluid Cells from a Neonate with Congenital Rubella

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