Growth of Mammalian Cells in a Heat-stable Dialysable Medium

Pumper, Robert W.; Yamashiroya, Herbert M.; Molander, L. T.

doi:10.1038/207662a0

Cited by 32 publications

(4 citation statements)

References 10 publications

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“…(11) Casein peptone dialysate (CPD). The preparation of CPD is based on a scaled-up version of the reverse dialysis technique employed by Pumper et al [15] for the production of a dialysate prepared from Difco Bacto Peptone [22]. Dissolve 60 g of casein digest peptone (BBL 97023) in 540 ml of water by vigorous stirring with a magnetic bar.…”

Section: Resultsmentioning

confidence: 99%

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A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba

Diamond

Harlow

Cunnick

1978

Transactions of the Royal Society of Tropical Medicine and Hygi

1,754

822

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We describe the first serum-free, partly defined medium (PDM-805) for cultivating the human enteric pathogen, Entamoeba histolytica, and the reptilian amebae E. barreti, E. invadens, and E. terrapinae. PDM-805 was developed by the stepwise replacement of yeast extract, bovine serum, and a casein peptone digest in TYI-S-33, a medium widely used for the axenic cultivation of these parasites. The defined components include amino acids, carbohydrates, B vitamins, ascorbic acid, tocopherol, thioctic acid, nucleic acid precursors, trace metals, and phosphate buffers. The undefined components include a highly purified bovine serum albumin, a lipoprotein-cholesterol solution from bovine serum, and a dialyzable, autoclavable, water-soluble growth factor(s) having a molecular weight of less than 3,500 prepared from casein peptone. To date, studies on the growth requirements of E. histolytica, strain 200:NIH, show the following are essential for sustained multiplication of this ameba: iron, glucose, biotin, folk acid, niacinamide, pantothenate, pyridoxal, riboflavin, thiamine, cysteine, an ammonium moiety (in addition to that present in cysteine), bovine serum albumin, lipoprotein-cholesterol, and casein peptone dialysate.

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Section: Resultsmentioning

confidence: 99%

“…Peptone dialysates have been used as serum substitutes for several mammalian cell lines [ 191. The most notable of these, Difco Bacto Peptone dialysate of Pumper et al, is water soluble and heat stable [15]. It is composed of glycine-rich peptides having estimated molecular weights below 2,000 [2 11.…”

Section: I-pdm-75mentioning

confidence: 99%

A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba

Diamond

Harlow

Cunnick

1978

Transactions of the Royal Society of Tropical Medicine and Hygi

1,754

822

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“…Each day 2 petri dishes were removed from each set and the cells dissociated from the monolayer with trypsin and counted. Where many cells were floating in the medium the cells in the medium were counted and the figures for attached and floating cells added to give the total cell 11.5 a 1.15 X 10' cells washed with CMF-PBS and suspended in 1 ml CMF-PBS were added to teach TC flask containing 20 ml MEM plus the additives shown.…”

Section: Growth Curves Of L-929 Cells On Serum Substitutesmentioning

confidence: 99%

Autoclavable low cost serum‐free cell culture media. The growth of L Cells and BHK cells on peptones

Keay

1975

Biotech & Bioengineering

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SummaryThe growth of L-929 cells on a series of peptones, and protein hydrolysates was examined, and it was found that when MEM was supplemented with any of a series of peptones, cell growth was about as good as when serum was used as a supplement. Protein hydrolysates did not support cell growth very well and at higher concentrations actually reduced cell growth. L-929 and L-6OTM cells were grown both as monolayers or stationary suspensions and in agitated systems in MEM supplemented with 0.5-la/, bactopeptone. The addition of macromolecular compounds, insulin or oleic acid had no effect on cell growth. BHK cells were also grown on media supplemented with bactopeptone but richer media (MEM-alpha, F-12, or RMPI1640) gave higher cell yields. The cells did not form the monolayers observed with fetal calf serum, but a partial suspension system. Addition of a detergent Darvan # 2 gave a totally suspension culture in both stationary and agitated systems.The production of Sindbis virus in BHK cells grown in serum-free media was examined and the yield of virus was found to be about the same as that produced in serum-supplemented systems. It is estimated that the cost of cell production media could be reduced by about 90% by the replacement of serum supplement by peptones.

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“…According to Pumper and Yamashiroya, between 50% and 80% of fetuses exposed to maternal rubella virus become infected prior to the 8 th weeks of gestation. Infection of the fetus is fairly uncommon, during the 3 rd trimester which is 6%–10% (3). The eyes, the ears, the heart, the central nervous system and the brain appear to be especially susceptible to rubella-associated damage (4).…”

Section: Introductionmentioning

confidence: 99%

Clinically confirmed congenital rubella syndrome: the role of echocardiography

Mekonnen

2017

Ethiop J Health Sci

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Growth of Mammalian Cells in a Heat-stable Dialysable Medium

Cited by 32 publications

References 10 publications

A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba

A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba

Autoclavable low cost serum‐free cell culture media. The growth of L Cells and BHK cells on peptones

Clinically confirmed congenital rubella syndrome: the role of echocardiography

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