Growth of Kabatiella Caulivora on Different Media

Colotelo, Nicholas; Grinchenko, A. H. Hertha

doi:10.1139/b62-044

Cited by 22 publications

(6 citation statements)

References 2 publications

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“…The nuclei in the hyphae of Tilletia species are difficult to stain, whereas the nuclei in the promycelium and primary sporidia are easy to stain. Many procedures (Clutterbuck and Roper, 1966;Colotelo and Grinchenko, 1962;Day and Jones, 1972;Furtado, 1970;Hawker, Abbot, and Gooday, 1968;Hirschhorn, 1945;Hrushovetz, 1956;Kao, 1956;Robinow, 1948;Rogers, 1965) were used in an attempt to stain the nuclei of the bunt fungi but the results were unsatisfactory. Many fungal hyphae grown on rich media contain a high level of lipids that tend to block good penetration of hydrophilic dyes.…”

Section: Results-mentioning

confidence: 99%

Morphology and Cytology of Tilletia caries and T. controversa in Axenic Culture

Trione

1974

American Journal of Botany

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On a wheat-based medium, the pathogenic phase of the common and dwarf bunt fungi grew slowly at 15-18 C and continued to produce massive quantities of teliospores in all subcultures for over 2 years. At warmer temperatures or on a chemically defined medium, the teliosporogenic colonies reverted to haploid mononucleate colonies. The hyphae of the teliosporogenic colonies were stained with a modified Giemsa technique and found to be thick, contorted, highly branched and short celled with usually one or two nuclei per cell. In contrast, the haploid mononucleate hyphae were thinner, straighter, and longer and never contained more than one nucleus per cell. The vegetative hyphae of T. caries and T. controversa were indistinguishable. Teliospores, formed at the terminal end of binucleate hyphae, were initially binucleate but became mononucleate before the mature cell wall formed.

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Section: Results-mentioning

confidence: 99%

Morphology and Cytology of Tilletia caries and T. controversa in Axenic Culture

Trione

1974

American Journal of Botany

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“…Samples for microscopic exatnination were taken from unmapped plates and nuclei were stained with HCI-Giemsa (Colotelo & Grinchenko, 1962).…”

Section: Methodsmentioning

confidence: 99%

Axenic culture of Peridermium pini from single aeciospores

Pei

Gibbs

1992

Plant Pathology

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For the first time with a rust fungus, continuously growing axenic cultures have been obtained from single spores. This was achieved with aeciospore sources of the pine stem rust Peridermium pini (Pers.) Lev. from Thetford Forest, East Anglia, but not with sources from north‐east Scotland. Approximately 30% of the single spores of two East Anglian sources out of the three tested developed into transferable and subculturable colonies on modified Harvey & Grasham's medium.

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“…Nuclei in spores were stained by the Giemsa procedure as described by Colotelo and Grinchenko (1962).…”

Section: Nuclear Stainingmentioning

confidence: 99%

Behavior of colorless appressoria in an albino mutant of Colletotrichum lagenarium

Suzuki¹,

Kubo²,

Furusawa³

et al. 1982

Can. J. Microbiol.

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of colorless appressoria in an albino mutant of Colletotrichum lagenarium. Can. J. Microbiol. 28: 1210-1213.Spores of an albino mutant of Colletotrichum lagenarium formed colorless appressoria. The appressoria germinated laterally and formed secondary appressoria after further incubation. Appressorial formation was accompanied by nuclear division and migration of the spore contents into the primary appressorium. When the primary appressorium was mature, the nucleus in the spore had disappeared and the spore appeared empty. Similar phenomena were observed in the formation of secondary appressoria from primary appressoria. Formation of both primary and secondary appressoria was temperature sensitive. When spores were incubated in the presence of cycloheximide after 1 h of incubation, formation of primary and secondary appressoria was not affected. Secondary appressoria pigmented by addition of 3,4-dihydroxyphenylalanine could penetrate nitrocellulose membranes. SUZUKI, K., Y. KUBO, I. FURUSAWA, N. ISHIDA et M. YAMAMOTO. 1982. Behavior of colorless appressoria in an albino mutant of Colletotrichum lagenarium. Can. J. Microbiol. 28: 1210-121 3.Les spores d'un mutant albinos de Colletotrichum lagenarium forment des appressoria incolores. Les appressoria germent latkralement et forment des appressoria secondaires aprks une incubation prolongCe. La formation des appressoria s'accompagne d'une division nuclkaire et le contenu des spores Cmigre dans l'appressorium primaire. Lors de la maturation de l'appressorium primaire, le noyau dans la spore disparait et la spore apparait vide. Des phCnomknes semblables ont Ct C observCs lors de la formation des appressoria secondaires i partir des appressoria primaires. La formation des appressoria primaires et secondaires est sensible i la tempCrature. La formation des appressoria primaires et secondaires, n'est pas affectke lorsque les spores sont incubCes en prksence de cycloheximide apks une heure d'incubation. Les appressoria secondaires qui forment des pigments par l'addition de 3,4-dihydroxyphknylalanine peuvent pknCtrer des membranes de nitro-cellulose.[Traduit par le journal]

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Growth of Kabatiella Caulivora on Different Media

Cited by 22 publications

References 2 publications

Morphology and Cytology of Tilletia caries and T. controversa in Axenic Culture

Morphology and Cytology of Tilletia caries and T. controversa in Axenic Culture

Axenic culture of Peridermium pini from single aeciospores

Behavior of colorless appressoria in an albino mutant of Colletotrichum lagenarium

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