Axenic culture of <i>Peridermium pini</i> from single aeciospores

Pei, M. H.; Gibbs, J. N.

doi:10.1111/j.1365-3059.1992.tb02323.x

Cited by 10 publications

(3 citation statements)

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“…The two forms of the fungus, previously separated on the basis of their germ-tube morphology and cytology (GIBBS et al 1988) have now been shown to possess a high degree of cultural identity When the results here are taken together with the discovery that single aeciospores from some Thetford origins can produce colonies, while those of Morayshire origin cannot (PEI and GIBBS 1991), it is clear that major genetical differences exist between the two forms of I? pini in the UK.…”

Section: Discussionmentioning

confidence: 88%

Cultural variation in Peridermium pini from two parts of the United Kingdom

Pei

Gibbs

1991

European Journal of Forest Pathology

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Axenic cultures of Peridermium ini obtained from aeciospores on diseased Pinus sylvestris in north-east Scotland t pically hacf an ill-defined margin and a fluffy surface, while those from East Anglia had a well-defined margin and appeared mucilaginous. The difference in colony appearance is consistent with other evidence for a distinction between the forms of Ppini in the two arts of the United Kingdom. After a few months, thin sectors, comprising a rapidly expanding gypha1 network, developed in some cultures from both spore sources.

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Section: Discussionmentioning

confidence: 88%

Cultural variation in Peridermium pini from two parts of the United Kingdom

Pei

Gibbs

1991

European Journal of Forest Pathology

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“…The stimulating effects of mass spores on mycelial growth have been observed in some rust fungi in attempts to establish axenic cultures from the spores. For example, P. graminis tritici (Bose & Shaw, 1971), Melampsora lini (Tuel, 1969) and Peridermium pini from Scotland (Pei & Gibbs, 1992) grew on artificial media only when mass spores were inoculated. Although the present results proved that each successful inoculation with a single spore resulted in the development of only one pustule, the high infection efficiency obtained in the experiment raises the question whether a single-spore infection can produce more than one pustule when more spores are applied.…”

Section: Discussionmentioning

confidence: 99%

Quantitative relationships between inoculum of Melampsora larici‐epitea and corresponding disease on Salix

Pei¹,

Ruiz²,

Hunter³

et al. 2002

Plant Pathology

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Six Salix clones were inoculated with urediniospores of four isolates of Melampsora larici-epitea at five inoculum levels using a leaf-disc method. Disease reactions were recorded using a digital camera; the number and size of uredinia were examined using image analysis software; and spore yield per leaf disc was measured. In three Salix / Melampsora combinations, S. × mollissima 'Q83'/Q1 (LET4); S. viminalis '78183'/ V1 (LET1); and S. × stipularis / V1, pustule numbers increased as inoculum density became higher. In the remainder, S. viminalis 'Mullatin'/ V1; S. × calodendron /DB (LET3); and S. burjatica 'Korso'/ K (LR1), pustule numbers initially increased, then decreased as inoculum densities exceeded 140 -360 spores per disc. Calculated infection efficiency ranged from 0·11 to 0·20 on the three willows inoculated with V1: 0·16 -0·68 for S. × calodendron / DB; 0·20 -0·55 for 'Q83'/Q1; and 0·07 -0·48 for Korso / K. In single-spore inoculations, up to 10% of spores produced single uredinia. Infection efficiency increased sharply between inoculum densities of 1-40 spores per leaf disc. Spore yield was more closely correlated to pustule area (accounting for 61·2% variance for the combined data) than to the number of pustules (42·7% variance). For spore yields in relation to pustule numbers, clone-specific individual lines having different intercepts and slopes fitted significantly better than either a single line for all the tested willows, or parallel lines fitted to each clone ( P < 0·001). For spore yields in relation to pustule area, clonespecific individual parallel lines were significantly better than a single line ( P < 0·001).

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“…(26) e Pucciniastrum sp. (27), Endocronartium harknessii (1), Peridermium pini (16), Cronartium flaccidum (13), Melampsora epitea (3), Melampsora larici-tremulae (14), Peridermium harknessii, Cronartium quercuum f. sp. banksinae e C. comandrae (24), Melampsoridium hiratsukanum (15).…”

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confidence: 99%

Estudos de três meios de cultura axênicos para a ferrugem das mirtáceas

et al. 2016

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Pizetta, M.; Pierozzi, C.G.; Pereira, G.V.N.; Cruz, J.C.S.; Aparecido, C.C.; Passador, M.M.; Furtado, E.L. Estudos de três meios de cultura axênicos para a ferrugem das mirtáceas. Summa Phytopathologica, v.42, n.2, p.165-169, 2016. Palavras-chave:Puccinia psidii, Myrtaceae, cultivo de biotrófico. RESUMO(21) e HG Harvey & Grasham (7), modificados. Comparativamente, elaboraram-se meios enriquecidos a partir de folhas de jambeiro (FJ), em quatro concentrações: 0,5; 1,0; 3,0 e 5,0 g L -1 (gramas de folhas para cada litro de água destilada). Neste estudo foi constatado apenas o primeiro estágio de crescimento fúngico, crescimento dos tubos germinativos, não apresentando continuação no desenvolvimento do patógeno. Os meios de cultura enriquecidos que propiciaram maior crescimento do tubo germinativo de P. psidii foram os meios ASZV, HG e FJ na concentração 0,5 g L -1 . ABSTRACTAxenic cultivation has been shown as an important tool for studies related to the biology, the pathogenicity and the maintenance of pure cultures of biotrophic species. Therefore, the present study aimed to analyze the in vitro growth of Puccinia psidii uredospores, using enriched culture media that had success in the axenic cultivation of other rusts. Compounds such as salts, amino acids, carbohydrates and vitamins were selected for the preparation of three enriched culture media: ASZV Kuck (10), SH Schenk & Hildebrandt (21) Pizetta, M.; Pierozzi, C.G.; Pereira, G.V.N.; Cruz, J.C.S.; Aparecido, C.C.; Passador, M.M.; Furtado, E.L. Study of three axenic culture media for rust in Myrtaceae. Summa Phytopathologica, v.42, n.2, p.165-169, 2016. and HG Harvey & Grasham (7), modified. Comparatively, enriched media were prepared from leaves of "jambeiro" (FJ) at four concentrations: 0.5; 1.0; 3.0 and 5.0 g L -1 (grams of leaves per one liter of distilled water). In this study, verification covered only the first stage of fungal growth, germ tube growth, not continuing in the pathogen development. The enriched media that provided greater P. psidii germ tube growth were the media ASZV, HG and FJ at 0.5 g L -1 concentration.

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Axenic culture of Peridermium pini from single aeciospores

Cited by 10 publications

References 10 publications

Cultural variation in Peridermium pini from two parts of the United Kingdom

Cultural variation in Peridermium pini from two parts of the United Kingdom

Quantitative relationships between inoculum of Melampsora larici‐epitea and corresponding disease on Salix

Estudos de três meios de cultura axênicos para a ferrugem das mirtáceas

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