Growth of Japanese Encephalitis Virus in Culex Tritaeniorhynchus Cell Cultures *

Hsu, Shan‐hui; Wang, B. T.; Huang, Mao-Hsiung; Wong, Wing-Yan; Cross, John H.

doi:10.4269/ajtmh.1975.24.881

Cited by 6 publications

(1 citation statement)

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“…Plaque production and fusion of cells by JE virus has been demonstrated in both invertebrate [6,12,19,25,27, 311 and vertebrate cell lines [24] but its significance in pathogenesis has not been clearly defined.…”

Section: Introductionmentioning

confidence: 99%

Differences in fusogenicity and mouse neurovirulence of Japanese encephalitis viruses

Higgs

Gould

1991

Archives of Virology

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The fusogenic capacity in AP-61 cell monolayers of 10 strains of Japanese encephalitis (JE) virus from different geographic locations was compared. One strain, isolated from Beijing (JE-Bei), did not fuse AP-61 cells after replication (fusion from within; FFWI), whereas all other strains fused these cells by 72 h post-infection. JE-Bei also readily established a non-cytolytic persistent infection in AP-61 cells. Differences in the envelope proteins of fusogenic and non-fusogenic virus were detected by haemagglutination-inhibition tests and by antigenic analysis using monoclonal antibodies. Yields of infectious virus in either AP-61 or Vero cell cultures were similar if JE-Bei was compared with the fusogenic strain (JE-Sar) but yields of haemagglutinin were 50-100 fold higher with the non-fusogenic virus, implying excessive generation of non-infectious particles. When added directly to AP-61 cell monolayers at pH6, only JE-Bei produced significant fusion from without (FFWO) presumably reflecting the larger quantity of antigen. Cell monolayers persistently infected with JE-Bei or monolayers treated with UV-inactivated JE-Bei, were resistant to superinfection with JE, West Nile and dengue 2 viruses but were susceptible to infection with the alphavirus Sindbis. When administered intracerebrally (I/C) to newborn and weanling mice, the viruses were equally neurovirulent. However, fusogenic JE-Sar was significantly more neurovirulent than JE-Bei for weanling mice after intraperitoneal (I/P) or subcutaneous (S/C) inoculation. Mice given non-fusogenic JE-Bei, resisted the peritoneal challenge with fusogenic JE-Sar, and West Nile but not Semliki Forest virus when given 6 h after the first virus. The potential significance of cell fusion by JE virus and interference through over production of non-infectious virus, is discussed in the context of JE virus virulence.

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Section: Introductionmentioning

confidence: 99%

Differences in fusogenicity and mouse neurovirulence of Japanese encephalitis viruses

Higgs

Gould

1991

Archives of Virology

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Establishment and characterization of a cell line from the mosquito Culex tritaeniorhynchus (Diptera: Culicidae)

Kuwata

Hoshino

Isawa

et al. 2012

In Vitro Cell.Dev.Biol.-Animal

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We established a continuous cell line from the embryo of the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae), a known major vector of the Japanese encephalitis virus (family Flaviviridae, genus Flavivirus) in Asia. The cell line, designated NIID-CTR, was serially subcultured in VP-12 medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS). It continued to grow for more than 60 passages over a 750-d period. The NIID-CTR cell line mainly comprised two morphologically distinct types of cells with adhesive properties: spindle-shaped and round cells. Most of the NIID-CTR cells at the 45th passage were diploid (2n = 6). The growth kinetics of the NIID-CTR cells was significantly affected by the FBS concentration in the medium. The population doubling time of the NIID-CTR cells was 20 h in the presence of 10 % FBS and 76 h in its absence. The DNA sequence of the mitochondrial cytochrome oxidase I gene confirmed that the NIID-CTR cell line was derived from C. tritaeniorhynchus. The cells were highly susceptible to Japanese encephalitis and Dengue viruses, thus providing a valuable tool for the study of mosquito-borne flaviviruses.

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Togaviruses in Cultured Arthropod Cells

Stollar¹

1980

The Togaviruses

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Growth of Japanese Encephalitis Virus in Culex Tritaeniorhynchus Cell Cultures *

Cited by 6 publications

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Differences in fusogenicity and mouse neurovirulence of Japanese encephalitis viruses

Differences in fusogenicity and mouse neurovirulence of Japanese encephalitis viruses

Establishment and characterization of a cell line from the mosquito Culex tritaeniorhynchus (Diptera: Culicidae)

Togaviruses in Cultured Arthropod Cells

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