Growth of ‘<i>Candidatus</i>Liberibacter asiaticus’ in Commercial Grapefruit Juice-Based Media Formulations Reveals Common Cell Density-Dependent Transient Behaviors

Silva, Marcus Vinicius Merfa E; Naranjo, Eber; Shantharaj, Deepak; Fuente, Leonardo De La

doi:10.1094/phyto-06-21-0228-fi

Cited by 6 publications

(1 citation statement)

References 79 publications

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“…However, L. crescens is a culturable species and is ancestral to all other described ‘ Ca. Liberibacter’ species ( Jain et al, 2019 ; Merfa et al, 2022 ). Due to their fastidious nature, obtaining genomic sequences of ‘ Ca.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

Batarseh

Morales‐Cruz

et al. 2023

Front. Microbiol.

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‘Candidatus Liberibacter’ is a group of bacterial species that are obligate intracellular plant pathogens and cause Huanglongbing disease of citrus trees and Zebra Chip in potatoes. Here, we examined the extent of intra- and interspecific genetic diversity across the genus using comparative genomics. Our approach examined a wide set of Liberibacter genome sequences including five pathogenic species and one species not known to cause disease. By performing comparative genomics analyses, we sought to understand the evolutionary history of this genus and to identify genes or genome regions that may affect pathogenicity. With a set of 52 genomes, we performed comparative genomics, measured genome rearrangement, and completed statistical tests of positive selection. We explored markers of genetic diversity across the genus, such as average nucleotide identity across the whole genome. These analyses revealed the highest intraspecific diversity amongst the ‘Ca. Liberibacter solanacearum’ species, which also has the largest plant host range. We identified sets of core and accessory genes across the genus and within each species and measured the ratio of nonsynonymous to synonymous mutations (dN/dS) across genes. We identified ten genes with evidence of a history of positive selection in the Liberibacter genus, including genes in the Tad complex, which have been previously implicated as being highly divergent in the ‘Ca. L. capsica’ species based on high values of dN.

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Section: Introductionmentioning

confidence: 99%

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

Batarseh

Morales‐Cruz

et al. 2023

Front. Microbiol.

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‘CandidatusLiberibacter’ Pathosystems at the Forefront of Agricultural and Biological Research Challenges

et al. 2022

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A Perspective on Current Therapeutic Molecule Screening Methods Against ‘Candidatus Liberibacter asiaticus’, the Presumed Causative Agent of Citrus Huanglongbing

Kennedy

Wood²,

Pitino³

et al. 2023

Phytopathology®

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Huanglongbing (HLB), referred to as citrus greening disease, is a bacterial disease impacting citrus production worldwide and fatal in young trees and certain varieties. In some areas, the disease is devastating the citrus industry. A successful solution to HLB will be measured in economics: Citrus growers need treatments that improve tree health, fruit production, and most importantly economic yield. The profitability of citrus groves is the ultimate metric that truly matters when searching for solutions to HLB. Scientific approaches used in the laboratory, greenhouse, or field trials are critical to discovery of those solutions and to estimate the likelihood of success of a treatment aimed for commercialization. Researchers and the citrus industry use a number of proxy evaluations of potential HLB solutions and understanding the strengths and limitations of each assay as well as how best to compare different assays is critical for decision making to advance therapies into field trials and commercialization. This perspective aims to help the reader compare and understand the limitations of different proxy evaluation systems based on the treatment and evaluation under consideration. The researcher must determine the suitability of one or more of these metrics to identify treatments and predict the usefulness of these treatments in having an eventual impact on citrus production and HLB mitigation. As therapies advance to field trials in the next few years, a re-evaluation of these metrics will be useful to guide future research efforts on strategies to mitigate HLB and vascular bacterial pathogens in other perennial crops.

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Growth of ‘CandidatusLiberibacter asiaticus’ in Commercial Grapefruit Juice-Based Media Formulations Reveals Common Cell Density-Dependent Transient Behaviors

Cited by 6 publications

References 79 publications

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

Comparative genomics of the Liberibacter genus reveals widespread diversity in genomic content and positive selection history

‘CandidatusLiberibacter’ Pathosystems at the Forefront of Agricultural and Biological Research Challenges

A Perspective on Current Therapeutic Molecule Screening Methods Against ‘Candidatus Liberibacter asiaticus’, the Presumed Causative Agent of Citrus Huanglongbing

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