‘<i>Candidatus</i>Liberibacter’ Pathosystems at the Forefront of Agricultural and Biological Research Challenges

Pierson, Elizabeth A.; Cubero, Jaime; Roper, M. Caroline; Brown, Judith K.; Bock, Clive H.; Wang, Nian

doi:10.1094/phyto-12-21-0497-fi

Cited by 4 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The symptoms are dependent of the host, but the most characteristics are leaf chlorosis and discoloration, wilting, twisted stems, swollen nodes and aerial tubers 7 , 27 , 28 , abnormal production of leaves and root sprouts, size reduction and other vegetative disorders 25 , 29 – 31 . In short, the commercial quality of the products is devalued, and the economic consequences are important 32 . CaLsol is distributed by America, Europe, North Africa, Israel and New Zealand.…”

Section: Introductionmentioning

confidence: 99%

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects

Chaves

Morán

Barbé

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Four pathogenic bacterial species of the genus ‘Candidatus Liberibacter’, transmitted by psyllid vectors, have been associated with serious diseases affecting economically important crops of Rutaceae, Apiaceae and Solanaceae families. The most severe disease of citrus plants, huanglongbing (HLB), is associated with ‘Ca. Liberibacter asiaticus’ (CaLas), ‘Ca. Liberibacter americanus’ (CaLam) and ‘Ca. Liberibacter africanus’ (CaLaf), while ‘Ca. Liberibacter solanacearum’ (CaLsol) is associated with zebra chip disease in potatoes and vegetative disorders in apiaceous plants. Since these bacteria remain non-culturable and their symptoms are non-specific, their detection and identification are done by molecular methods, mainly based on PCR protocols. In this study, a new quantitative real-time PCR protocol based on TaqMan probe, which can also be performed in a conventional PCR version, has been developed to detect the four known phytopathogenic species of the genus Liberibacter. The new protocol has been validated according to European Plant Protection Organization (EPPO) guidelines and is able to detect CaLas, CaLam, CaLaf and CaLsol in both plants and vectors, not only using purified DNA but also using crude extracts of potato and citrus or psyllids. A comparative analysis with other previously described qPCR protocols revealed that this new one developed in this study is more specific and equally or more sensitive. Thus, other genus-specific qPCR protocols have important drawbacks regarding the lack of specificity, while with the new protocol there was no cross-reactions in 250 samples from 24 different plant and insect species from eight different geographical origins. Therefore, it can be used as a rapid and time-saving screening test, as it allows simultaneous detection of all plant pathogenic species of ‘Ca. Liberibacter’ in a one-step assay.

show abstract

Section: Introductionmentioning

confidence: 99%

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects

Chaves

Morán

Barbé

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Phloem-restricted gram-negative bacterium Ca. Liberibacter species are associated with economically important plant diseases (Pierson et al 2022). Three species of Ca.…”

Section: Consequences Of Globalization and Challenges For Agriculturementioning

confidence: 99%

Dispersal behaviour, ecology and control of Philaenus spumarius : the main vector of Xylella fastidiosa in Europe

Blasco¹

View full text Add to dashboard Cite

por abrirme el camino para empezar a trabajar en el centro.Marina, la que ha sido una inspiración y un apoyo constante desde que entré en el laboratorio. Has sido una gran mentora y compañera. Te he echado de menos en esta última etapa.Gracias a todas mis compañeras del laboratorio. María, por el trabajo codo con codo en el campo hasta en los terrenos más hostiles, por las charlas infinitas y por todo tu trabajo en el laboratorio, sin el cual investigar no hubiera sido posible. Elisa, por tu paciencia infinita y por ayudarme a poner a punto el flight-mill. Borja, siempre cuidando de mis Sonchus. Marcos, por los miles de horas cuidando Philaenus y trabajando en el campo hasta la extenuación, además de un compañero me llevo un amigo. Ana, corriendo aventuras juntas desde

show abstract

Simplified extraction protocol of citrus tissues for rapid detection of ‘Candidatus Liberibacter asiaticus’ using isothermal recombinase polymerase amplification assay and its application for prevalence studies

Diksha,

Sharma,

Wangkhem

et al. 2024

South African Journal of Botany

View full text Add to dashboard Cite

‘CandidatusLiberibacter’ Pathosystems at the Forefront of Agricultural and Biological Research Challenges

Cited by 4 publications

References 31 publications

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects

A new and accurate qPCR protocol to detect plant pathogenic bacteria of the genus ‘Candidatus Liberibacter’ in plants and insects

Dispersal behaviour, ecology and control of Philaenus spumarius : the main vector of Xylella fastidiosa in Europe

Simplified extraction protocol of citrus tissues for rapid detection of ‘Candidatus Liberibacter asiaticus’ using isothermal recombinase polymerase amplification assay and its application for prevalence studies

Contact Info

Product

Resources

About