Growth of <i>Artemisia annua</i> hairy roots in liquid‐ and gas‐phase reactors

Kim, Yoo Jeong; Weathers, Pamela J.; Wyslouzil, Barbara E.

doi:10.1002/bit.10389

Cited by 62 publications

(56 citation statements)

References 32 publications

(41 reference statements)

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“…In a series of experiments in our lab (Kim et al, 2002), roots grown in a bubble column reactor consistently had higher dry mass compared to those harvested from a concurrent mist reactor. To explain this result, Kim et al (2002) compared the biological demand of the growing roots in the mist reactor to the level of substrate (sugar) deposited on the roots as a function of reactor packing fraction, where the deposition of medium droplets was calculated using a standard filter model (Wyslouzil et al, 1997).…”

Section: Introductionmentioning

confidence: 94%

Using an aerosol deposition model to increase hairy root growth in a mist reactor

Towler

Wyslouzil

Weathers

2006

Biotech & Bioengineering

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Gas-phase reactors, including the mist reactor, have distinct advantages over liquid-phase reactors including the ability to manipulate the gas composition, to allow effective gas exchange in a densely growing biomass, and to affect secondary metabolite production. Mathematical modeling suggested that roots in a mist reactor are often too sparsely packed to capture mist particles efficiently and cannot, therefore, meet the nutrient demands required to maintain high growth rates. Indeed, growth rates of Artemisia annua hairy roots increased significantly when the initial packing density increased or when a higher sucrose concentration was used in the medium. Growth kinetics for 2, 4, and 6 days, however, showed a decrease or stationary growth rate after only 4 days for both 3 and 5% sucrose feeds. Residual medium analyses indicated that carbon was not exhausted, nor were any of the other major nutrients including phosphate. Increasing the mist duty cycle at constant carbon flux through the reactor reduced the growth rates slightly. In general, the aerosol deposition model correctly predicted how to optimize hairy root growth in the mist reactor.

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Section: Introductionmentioning

confidence: 94%

Using an aerosol deposition model to increase hairy root growth in a mist reactor

Towler

Wyslouzil

Weathers

2006

Biotech & Bioengineering

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“…A number of elicitors such as methyl jasmonate (MJ) [14][15][16][17] and salicylic acid (SA) [14,18] have been used successfully for enhanced production of secondary metabolites in hairy root cultures of many plant species. In addition, fungal elicitors are significant in triggering phytoalexin production in cultured cells and have been used for enhanced production of secondary metabolites in some medicinal plants [19][20][21][22]. High biosynthetic capability of valepotriate production has been reported in hairy root cultures of Valeriana wallichii DC [23], Valerianella discoidea L. Loisel [24], Valeriana officinalis L. var.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Torkamani

Jafari²,

Abbaspour

et al. 2014

Open Life Sciences

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Valerenic acid (VA) is a pharmacologically-active sesquiterpene found in valerian (Valeriana officinalis L., Valerianaceae) roots and rhizomes. The plant produces only small amounts of this metabolite naturally. So, induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, Wild-type strain ‘A13’ of Agrobacterium rhizogenes was used to induce hairy roots in valerian. The influence of three different elicitors including Fusarium graminearum extract (FE), methyl jasmonate (MJ) and salicylic acid (SA) on VA production in the selected hairy root line ‘LeVa-C4’ was also investigated. The 23-day-old cultures were treated with different concentrations of the elicitors at exposure time of 3 and 7 days. FE (1%) and MJ (100 µM L−1) highly promoted VA production at 7 days after elicitation, to a level of 12.31- and 6-fold higher than that of non-elicited controls, respectively, and FE did not exert any negative effects on biomass yield of hairy root. SA did not significantly increase the production of VA. This is the first time study to assess the elicitation of hairy root cultures to promote VA biosynthesis in valerian and the resulting experiments demonstrated that F. graminearum extract and MJ were indeed a potent inducer of VA biosynthesis.

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“…Agrobacterium rhizogenes-mediated hairy root (HR) cultures represent an attractive experimental system for the production of high-value secondary metabolites, including pharmaceuticals and other biologically active substances of commercial importance [6,7]. Namely, HR cultures may synthesize higher levels of secondary metabolites or amounts comparable to those of the intact plant and offer a promising approach to the production of novel metabolites [8]. The first step towards the application of transformation procedures to few Hypericum species has been encountered.…”

Section: Introductionmentioning

confidence: 99%

Hairy roots of Hypericum perforatum L.: a promising system for xanthone production

et al. 2013

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Hypericum perforatum L. is a common perennial plant with a reputed medicinal value. Investigations have been made to develop an efficient protocol for the identification and quantification of secondary metabolites in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific primers. HR had an altered phenolic profile with respect to phenolic acids, flavonol glycosides, flavan-3-ols, flavonoid aglycones and xanthones comparing to control roots. Phenolics in control and HR cultures were observed to be qualitatively and quantitatively distinct. Quinic acid was the only detectable phenolic acid in HR. Transgenic roots are capable of producing flavonol glycosides such as quercetin 6-C-glucoside, quercetin 3-O-rutinoside (rutin) and isorhamnetin O-hexoside. The HPLC analysis of flavonoid aglycones in HR resulted in the identification of kaempferol. Transformed roots yielded higher levels of catechin and epicatechin than untransformed roots. Among the twenty-eight detected xanthones, four of them were identified as 1,3,5,6-tetrahydroxyxanthone, 1,3,6,7-tetrahydroxyxanthone, γ-mangostin and garcinone C were de novo synthesized in HR. Altogether, these results indicated that H. perforatum HR represent a promising experimental system for enhanced production of xanthones.

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Growth of Artemisia annua hairy roots in liquid‐ and gas‐phase reactors

Cited by 62 publications

References 32 publications

Using an aerosol deposition model to increase hairy root growth in a mist reactor

Using an aerosol deposition model to increase hairy root growth in a mist reactor

Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

Hairy roots of Hypericum perforatum L.: a promising system for xanthone production

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